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Phenotypical characterization of the peripheral blood T cells in patients with celiac disease: does it differentiate suspicious celiac disease cases?

Nataj Arab HH, Masjedi M, Alsahebfosoul F, Mokhtari M, Jamali N, Emami MH, Saffaei A - Gastroenterol Hepatol Bed Bench (2015)

Bottom Line: The mean age of patients was 33.6 ± 3.4 years and two patients had Marsh IIIB, five patients had Marsh IIIA and six patients had Marsh II histology class.The mean percentages of the TCR(+) T cells in the patients were significantly higher than the controls (p=0.015).However, the mean percentages of the αβTCR(+) T cells were significantly lower in the untreated patients than the controls (p=0.025).

View Article: PubMed Central - PubMed

Affiliation: Mazandaran University of Medical Sciences, Sari, Iran.

ABSTRACT

Aim: The present study aimed to study the immunological changes seen in the intestinal epithelium of the celiac patients could also be detected in the peripheral blood lymphocyte populations.

Background: Celiac disease (CD) is a small bowel enteropathy caused by permanent wheat gluten intolerance. One of the earliest signs of CD is an increase in the numbers of the intestinal intraepithelial lymphocytes (iIEL).

Patients and methods: In this case-control study, totally 13 untreated subjects with acceptable criteria for CD without any complication and 16 healthy subjects without any positive criteria for CD were selected. Peripheral blood T cells were analyzed by two-color flow cytometry in both groups.

Results: The mean age of patients was 33.6 ± 3.4 years and two patients had Marsh IIIB, five patients had Marsh IIIA and six patients had Marsh II histology class. The mean percentages of the TCR(+) T cells in the patients were significantly higher than the controls (p=0.015). However, the mean percentages of the αβTCR(+) T cells were significantly lower in the untreated patients than the controls (p=0.025). There were no significant difference between the mean percentages of lymphocytes expressing the CD3, CD4 and CD8 molecules in the patients and the controls.

Conclusion: The change in the percentages of the peripheral blood T cells expressing the γδTCR and αβTCR in the celiac patients could be used in conjunction with the other serological markers to identify new CD cases.

No MeSH data available.


Related in: MedlinePlus

Frequency of the αβTCR and γδTCR expression on the T cells, CD3+, CD4+, CD8+, CD4+/CD8+ ratio T lymphocyte subsets in the peripheral blood of the celiac patients and controls
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Figure 1: Frequency of the αβTCR and γδTCR expression on the T cells, CD3+, CD4+, CD8+, CD4+/CD8+ ratio T lymphocyte subsets in the peripheral blood of the celiac patients and controls

Mentions: Furthermore, the proportions of blood lymphocytes expressing T cell markers and ratios of CD4/CD8 T cells are summarized in table 1 and figure 1. The Kolmogorov–Smirnov test shows the distributions of data are normal (p>0.05). The levels of CD3+, CD4+ and CD8+ T cells weren’t equal, but there were no significant differences in the percentages of these cells in the CD patients and healthy controls (Table 1). The ratio of CD4+/CD8+ T cells (helper/suppressor ratio) was also calculated, and we found no significant difference between untreated CD patients and healthy controls (1.60 ± 0.12 vs. 1.83 ± 0.42; P=0.198). However, the mean percentages of the circulating TCR+ T cells in the patients were significantly higher than the controls (7.58 ± 1.15% vs. 4.44 ± .052%; p = 0.014). Conversely, the mean percentages of the circulating αβTCR+ T cells were significantly lower in the untreated patients than the controls (89.74 ± 1.05% vs. 93.64 ± 0.62%, P = 0.003).


Phenotypical characterization of the peripheral blood T cells in patients with celiac disease: does it differentiate suspicious celiac disease cases?

Nataj Arab HH, Masjedi M, Alsahebfosoul F, Mokhtari M, Jamali N, Emami MH, Saffaei A - Gastroenterol Hepatol Bed Bench (2015)

Frequency of the αβTCR and γδTCR expression on the T cells, CD3+, CD4+, CD8+, CD4+/CD8+ ratio T lymphocyte subsets in the peripheral blood of the celiac patients and controls
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4403025&req=5

Figure 1: Frequency of the αβTCR and γδTCR expression on the T cells, CD3+, CD4+, CD8+, CD4+/CD8+ ratio T lymphocyte subsets in the peripheral blood of the celiac patients and controls
Mentions: Furthermore, the proportions of blood lymphocytes expressing T cell markers and ratios of CD4/CD8 T cells are summarized in table 1 and figure 1. The Kolmogorov–Smirnov test shows the distributions of data are normal (p>0.05). The levels of CD3+, CD4+ and CD8+ T cells weren’t equal, but there were no significant differences in the percentages of these cells in the CD patients and healthy controls (Table 1). The ratio of CD4+/CD8+ T cells (helper/suppressor ratio) was also calculated, and we found no significant difference between untreated CD patients and healthy controls (1.60 ± 0.12 vs. 1.83 ± 0.42; P=0.198). However, the mean percentages of the circulating TCR+ T cells in the patients were significantly higher than the controls (7.58 ± 1.15% vs. 4.44 ± .052%; p = 0.014). Conversely, the mean percentages of the circulating αβTCR+ T cells were significantly lower in the untreated patients than the controls (89.74 ± 1.05% vs. 93.64 ± 0.62%, P = 0.003).

Bottom Line: The mean age of patients was 33.6 ± 3.4 years and two patients had Marsh IIIB, five patients had Marsh IIIA and six patients had Marsh II histology class.The mean percentages of the TCR(+) T cells in the patients were significantly higher than the controls (p=0.015).However, the mean percentages of the αβTCR(+) T cells were significantly lower in the untreated patients than the controls (p=0.025).

View Article: PubMed Central - PubMed

Affiliation: Mazandaran University of Medical Sciences, Sari, Iran.

ABSTRACT

Aim: The present study aimed to study the immunological changes seen in the intestinal epithelium of the celiac patients could also be detected in the peripheral blood lymphocyte populations.

Background: Celiac disease (CD) is a small bowel enteropathy caused by permanent wheat gluten intolerance. One of the earliest signs of CD is an increase in the numbers of the intestinal intraepithelial lymphocytes (iIEL).

Patients and methods: In this case-control study, totally 13 untreated subjects with acceptable criteria for CD without any complication and 16 healthy subjects without any positive criteria for CD were selected. Peripheral blood T cells were analyzed by two-color flow cytometry in both groups.

Results: The mean age of patients was 33.6 ± 3.4 years and two patients had Marsh IIIB, five patients had Marsh IIIA and six patients had Marsh II histology class. The mean percentages of the TCR(+) T cells in the patients were significantly higher than the controls (p=0.015). However, the mean percentages of the αβTCR(+) T cells were significantly lower in the untreated patients than the controls (p=0.025). There were no significant difference between the mean percentages of lymphocytes expressing the CD3, CD4 and CD8 molecules in the patients and the controls.

Conclusion: The change in the percentages of the peripheral blood T cells expressing the γδTCR and αβTCR in the celiac patients could be used in conjunction with the other serological markers to identify new CD cases.

No MeSH data available.


Related in: MedlinePlus