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Intraoperative Optical Coherence Tomography Using the RESCAN 700: Preliminary Results in Collagen Crosslinking.

Pahuja N, Shetty R, Jayadev C, Nuijts R, Hedge B, Arora V - Biomed Res Int (2015)

Bottom Line: The mean corneal hyperreflectance in the epi-on group was 12.97 ± 1.49 gray scale units (GSU) before instillation of riboflavin and 14.46 ± 2.09 GSU after AXCL (P = 0.019) while in the epi-off group it was 11.43 ± 2.68 GSU and 16.98 ± 8.49 GSU, respectively (P = 0.002).The average depth of the band of hyperreflectance in the epi-on group was 149.39 ± 15.63 microns and in the epi-off group it was 191.04 ± 32.18 microns.This novel in vivo, real time imaging study demonstrates riboflavin penetration during epi-on and epi-off ACXL.

View Article: PubMed Central - PubMed

Affiliation: Department of Cornea & Refractive Surgery, Narayana Nethralaya 121/C, Chord Road, 1st "R" Block, Rajajinagar, Bangalore, Karnataka 560 010, India.

ABSTRACT

Purpose: To compare the penetration of riboflavin using a microscope-integrated real time spectral domain optical coherence tomography (ZEISS OPMI LUMERA 700 and ZEISS RESCAN 700) in keratoconus patients undergoing accelerated collagen crosslinking (ACXL) between epithelium on (epi-on) and epithelium off (epi-off).

Methods: Intraoperative images were obtained during each of the procedures. Seven keratoconus patients underwent epi-on ACXL and four underwent epi-off ACXL. A software tool was developed using Microsoft.NET and Open Computer Vision (OpenCV) libraries for image analysis. Pre- and postprocedure images were analyzed for changes in the corneal hyperreflectance pattern as a measure of the depth of riboflavin penetration.

Results: The mean corneal hyperreflectance in the epi-on group was 12.97 ± 1.49 gray scale units (GSU) before instillation of riboflavin and 14.46 ± 2.09 GSU after AXCL (P = 0.019) while in the epi-off group it was 11.43 ± 2.68 GSU and 16.98 ± 8.49 GSU, respectively (P = 0.002). The average depth of the band of hyperreflectance in the epi-on group was 149.39 ± 15.63 microns and in the epi-off group it was 191.04 ± 32.18 microns.

Conclusion: This novel in vivo, real time imaging study demonstrates riboflavin penetration during epi-on and epi-off ACXL.

No MeSH data available.


Related in: MedlinePlus

Total reflectance (sum of all gray values) seen in patients undergoing epi-on ACXL before and after the procedure.
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fig1: Total reflectance (sum of all gray values) seen in patients undergoing epi-on ACXL before and after the procedure.

Mentions: The total reflectance (sum of all gray values) in the epi-on group (Figure 1) was 4386.13 ± 497.38 gray scale units (GSU) before ACXL and 4888.03 ± 387.62 GSU after ACXL and the average reflectance (Figure 2) was 12.97 ± 1.49 GSU before starting the procedure and 14.46 ± 2.09 GSU after ACXL (P = 0.019). The total reflectance in the epi-off group (Figure 3) was 4664.0 ± 1094.88 GSU and 6767.0 ± 805.74 GSU before and after the procedure, respectively. The mean corneal hyperreflectance (Figure 4) in the same group was 11.43 ± 2.68 GSU and 16.98 ± 8.49 GSU before and after ACXL, respectively (P = 0.002). The mean of the ratio of the anterior two-thirds and posterior one-third of cornea in epi-on group before starting of the procedure was 51.95 ± 10.22 GSU and after ACXL was 57.34 ± 10.23 GSU (P = 0.035) and in the epi-off group it was 44.63 ± 16.98 GSU and 64.63 ± 7.21 GSU before and at the end ACXL, respectively (P = 0.034).


Intraoperative Optical Coherence Tomography Using the RESCAN 700: Preliminary Results in Collagen Crosslinking.

Pahuja N, Shetty R, Jayadev C, Nuijts R, Hedge B, Arora V - Biomed Res Int (2015)

Total reflectance (sum of all gray values) seen in patients undergoing epi-on ACXL before and after the procedure.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4402560&req=5

fig1: Total reflectance (sum of all gray values) seen in patients undergoing epi-on ACXL before and after the procedure.
Mentions: The total reflectance (sum of all gray values) in the epi-on group (Figure 1) was 4386.13 ± 497.38 gray scale units (GSU) before ACXL and 4888.03 ± 387.62 GSU after ACXL and the average reflectance (Figure 2) was 12.97 ± 1.49 GSU before starting the procedure and 14.46 ± 2.09 GSU after ACXL (P = 0.019). The total reflectance in the epi-off group (Figure 3) was 4664.0 ± 1094.88 GSU and 6767.0 ± 805.74 GSU before and after the procedure, respectively. The mean corneal hyperreflectance (Figure 4) in the same group was 11.43 ± 2.68 GSU and 16.98 ± 8.49 GSU before and after ACXL, respectively (P = 0.002). The mean of the ratio of the anterior two-thirds and posterior one-third of cornea in epi-on group before starting of the procedure was 51.95 ± 10.22 GSU and after ACXL was 57.34 ± 10.23 GSU (P = 0.035) and in the epi-off group it was 44.63 ± 16.98 GSU and 64.63 ± 7.21 GSU before and at the end ACXL, respectively (P = 0.034).

Bottom Line: The mean corneal hyperreflectance in the epi-on group was 12.97 ± 1.49 gray scale units (GSU) before instillation of riboflavin and 14.46 ± 2.09 GSU after AXCL (P = 0.019) while in the epi-off group it was 11.43 ± 2.68 GSU and 16.98 ± 8.49 GSU, respectively (P = 0.002).The average depth of the band of hyperreflectance in the epi-on group was 149.39 ± 15.63 microns and in the epi-off group it was 191.04 ± 32.18 microns.This novel in vivo, real time imaging study demonstrates riboflavin penetration during epi-on and epi-off ACXL.

View Article: PubMed Central - PubMed

Affiliation: Department of Cornea & Refractive Surgery, Narayana Nethralaya 121/C, Chord Road, 1st "R" Block, Rajajinagar, Bangalore, Karnataka 560 010, India.

ABSTRACT

Purpose: To compare the penetration of riboflavin using a microscope-integrated real time spectral domain optical coherence tomography (ZEISS OPMI LUMERA 700 and ZEISS RESCAN 700) in keratoconus patients undergoing accelerated collagen crosslinking (ACXL) between epithelium on (epi-on) and epithelium off (epi-off).

Methods: Intraoperative images were obtained during each of the procedures. Seven keratoconus patients underwent epi-on ACXL and four underwent epi-off ACXL. A software tool was developed using Microsoft.NET and Open Computer Vision (OpenCV) libraries for image analysis. Pre- and postprocedure images were analyzed for changes in the corneal hyperreflectance pattern as a measure of the depth of riboflavin penetration.

Results: The mean corneal hyperreflectance in the epi-on group was 12.97 ± 1.49 gray scale units (GSU) before instillation of riboflavin and 14.46 ± 2.09 GSU after AXCL (P = 0.019) while in the epi-off group it was 11.43 ± 2.68 GSU and 16.98 ± 8.49 GSU, respectively (P = 0.002). The average depth of the band of hyperreflectance in the epi-on group was 149.39 ± 15.63 microns and in the epi-off group it was 191.04 ± 32.18 microns.

Conclusion: This novel in vivo, real time imaging study demonstrates riboflavin penetration during epi-on and epi-off ACXL.

No MeSH data available.


Related in: MedlinePlus