Epstein-Barr virus transcription factor Zta acts through distal regulatory elements to directly control cellular gene expression.
Bottom Line: Using genome-wide chromatin immunoprecipitation coupled to next-generation DNA sequencing (ChIP-seq) we established a map of Zta interactions across the human genome.Zta binds 278 of the regulated genes and the distribution of binding sites shows that Zta binds mostly to sites that are distal to transcription start sites.This differs from the prevailing view that Zta activates viral genes by binding exclusively at promoter elements.
Affiliation: School of Life Sciences, University of Sussex, Brighton BN1 9QG, UK.Show MeSH
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Mentions: Although some of the Zta binding sites (14%) were close to the annotated transcriptional start sites (TSS) of cellular genes (Figure 1B and Supplementary Table 1), analogous to the situation observed for the interaction of Zta with viral genes, the majority of the Zta binding sites (86%) were more distant (≥4 kb from the nearest TSS). By assigning each of the Zta peaks to the closest TSS, irrespective of distance, we identified 2395 cellular genes as potential candidates for regulation by Zta.
Affiliation: School of Life Sciences, University of Sussex, Brighton BN1 9QG, UK.