The nuclease FAN1 is involved in DNA crosslink repair in Arabidopsis thaliana independently of the nuclease MUS81.
Bottom Line: No FAN1 homolog is present in Drosophila and Saccharomyces cerevisiae.Both the virus-type replication-repair nuclease and the ubiquitin-binding ubiquitin-binding zinc finger domains are essential for this function.Mutations in both FAN1 and the endonuclease MUS81 resulted in greater sensitivity against CLs than in the respective single mutants.
Affiliation: Botanical Institute II, Karlsruhe Institute of Technology, Hertzstrasse 16, Karlsruhe, 76187, Germany.Show MeSH
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Mentions: To analyze if FAN1 plays a role in the repair of interstrand CLs in Arabidopsis, we tested the fan1-1 mutant line for hypersensitivity against the interstrand CL-inducing agent MMC (45). AtFAN1 seems to be involved in the repair of interstrand CLs, as the single mutant showed an increased sensitivity against MMC (Figure 3A). To test whether FAN1 has a more general role in DNA repair in Arabidopsis, we tested the sensitivity of the fan1-1 mutant against bleomycin, cis-Platin, hydroxyurea, methyl methanesulfonate and camptothecin. However, none of these treatments revealed any hypersensitivity (Supplementary Figure S3).
Affiliation: Botanical Institute II, Karlsruhe Institute of Technology, Hertzstrasse 16, Karlsruhe, 76187, Germany.