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The nuclease FAN1 is involved in DNA crosslink repair in Arabidopsis thaliana independently of the nuclease MUS81.

Herrmann NJ, Knoll A, Puchta H - Nucleic Acids Res. (2015)

Bottom Line: No FAN1 homolog is present in Drosophila and Saccharomyces cerevisiae.Both the virus-type replication-repair nuclease and the ubiquitin-binding ubiquitin-binding zinc finger domains are essential for this function.Mutations in both FAN1 and the endonuclease MUS81 resulted in greater sensitivity against CLs than in the respective single mutants.

View Article: PubMed Central - PubMed

Affiliation: Botanical Institute II, Karlsruhe Institute of Technology, Hertzstrasse 16, Karlsruhe, 76187, Germany.

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Sensitivity of Atfan1–1 and the fan1-1::FAN1 WT complementation lines after MMC treatment. To calculate relative fresh weights of the tested lines, the absolute fresh weights of MMC-treated plants were normalized with fresh weights of untreated control plants from identical lines. Each assay was performed at least three times to calculate mean values and standard deviations (error bars). (A) Compared to WT plants, the Atfan1-1 mutant showed a reduced relative fresh weight after MMC treatment. (B) The fan1-1::FAN1 WT complementation lines #1, #2, #3 and #4 were able to complement the increased sensitivity of fan1-1 after MMC treatment. P-value ≤ 0.05 (*); P-value < 0.01 (**); P-value < 0.001 (***).
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Figure 3: Sensitivity of Atfan1–1 and the fan1-1::FAN1 WT complementation lines after MMC treatment. To calculate relative fresh weights of the tested lines, the absolute fresh weights of MMC-treated plants were normalized with fresh weights of untreated control plants from identical lines. Each assay was performed at least three times to calculate mean values and standard deviations (error bars). (A) Compared to WT plants, the Atfan1-1 mutant showed a reduced relative fresh weight after MMC treatment. (B) The fan1-1::FAN1 WT complementation lines #1, #2, #3 and #4 were able to complement the increased sensitivity of fan1-1 after MMC treatment. P-value ≤ 0.05 (*); P-value < 0.01 (**); P-value < 0.001 (***).

Mentions: To analyze if FAN1 plays a role in the repair of interstrand CLs in Arabidopsis, we tested the fan1-1 mutant line for hypersensitivity against the interstrand CL-inducing agent MMC (45). AtFAN1 seems to be involved in the repair of interstrand CLs, as the single mutant showed an increased sensitivity against MMC (Figure 3A). To test whether FAN1 has a more general role in DNA repair in Arabidopsis, we tested the sensitivity of the fan1-1 mutant against bleomycin, cis-Platin, hydroxyurea, methyl methanesulfonate and camptothecin. However, none of these treatments revealed any hypersensitivity (Supplementary Figure S3).


The nuclease FAN1 is involved in DNA crosslink repair in Arabidopsis thaliana independently of the nuclease MUS81.

Herrmann NJ, Knoll A, Puchta H - Nucleic Acids Res. (2015)

Sensitivity of Atfan1–1 and the fan1-1::FAN1 WT complementation lines after MMC treatment. To calculate relative fresh weights of the tested lines, the absolute fresh weights of MMC-treated plants were normalized with fresh weights of untreated control plants from identical lines. Each assay was performed at least three times to calculate mean values and standard deviations (error bars). (A) Compared to WT plants, the Atfan1-1 mutant showed a reduced relative fresh weight after MMC treatment. (B) The fan1-1::FAN1 WT complementation lines #1, #2, #3 and #4 were able to complement the increased sensitivity of fan1-1 after MMC treatment. P-value ≤ 0.05 (*); P-value < 0.01 (**); P-value < 0.001 (***).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4402529&req=5

Figure 3: Sensitivity of Atfan1–1 and the fan1-1::FAN1 WT complementation lines after MMC treatment. To calculate relative fresh weights of the tested lines, the absolute fresh weights of MMC-treated plants were normalized with fresh weights of untreated control plants from identical lines. Each assay was performed at least three times to calculate mean values and standard deviations (error bars). (A) Compared to WT plants, the Atfan1-1 mutant showed a reduced relative fresh weight after MMC treatment. (B) The fan1-1::FAN1 WT complementation lines #1, #2, #3 and #4 were able to complement the increased sensitivity of fan1-1 after MMC treatment. P-value ≤ 0.05 (*); P-value < 0.01 (**); P-value < 0.001 (***).
Mentions: To analyze if FAN1 plays a role in the repair of interstrand CLs in Arabidopsis, we tested the fan1-1 mutant line for hypersensitivity against the interstrand CL-inducing agent MMC (45). AtFAN1 seems to be involved in the repair of interstrand CLs, as the single mutant showed an increased sensitivity against MMC (Figure 3A). To test whether FAN1 has a more general role in DNA repair in Arabidopsis, we tested the sensitivity of the fan1-1 mutant against bleomycin, cis-Platin, hydroxyurea, methyl methanesulfonate and camptothecin. However, none of these treatments revealed any hypersensitivity (Supplementary Figure S3).

Bottom Line: No FAN1 homolog is present in Drosophila and Saccharomyces cerevisiae.Both the virus-type replication-repair nuclease and the ubiquitin-binding ubiquitin-binding zinc finger domains are essential for this function.Mutations in both FAN1 and the endonuclease MUS81 resulted in greater sensitivity against CLs than in the respective single mutants.

View Article: PubMed Central - PubMed

Affiliation: Botanical Institute II, Karlsruhe Institute of Technology, Hertzstrasse 16, Karlsruhe, 76187, Germany.

Show MeSH
Related in: MedlinePlus