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Shuang-huang-lian attenuates lipopolysaccharide-induced acute lung injury in mice involving anti-inflammatory and antioxidative activities.

Fang L, Gao Y, Liu F, Hou R, Cai RL, Qi Y - Evid Based Complement Alternat Med (2015)

Bottom Line: Histological studies demonstrated that SHL attenuated LPS-induced interstitial edema, hemorrhage, and the infiltration of neutrophils into the lung tissue.Moreover, SHL could also enhance the superoxide dismutase and catalase activities, increase the reduced glutathione content, and decrease the malondialdehyde content.The present results suggest that SHL possesses anti-inflammatory and antioxidative properties that may protect mice against LPS-induced ALI.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.

ABSTRACT
Shuang-Huang-Lian (SHL) is a common traditional Chinese preparation extracted from Lonicerae Japonicae Flos, Scutellariae Radix, and Fructus Forsythiae. In this study, we demonstrate the anti-inflammatory and antioxidative effects of SHL on lipopolysaccharide- (LPS-) induced acute lung injury (ALI) in mice. SHL reduced the lung wet/dry weight ratio, lowered the number of total cells in the bronchoalveolar lavage fluid, and decreased the myeloperoxidase activity in lung tissues 6 h after LPS treatment. It also inhibited the overproduction of proinflammatory cytokines (TNF-α, IL-1β, and IL-6) in the bronchoalveolar lavage fluid. Histological studies demonstrated that SHL attenuated LPS-induced interstitial edema, hemorrhage, and the infiltration of neutrophils into the lung tissue. Moreover, SHL could also enhance the superoxide dismutase and catalase activities, increase the reduced glutathione content, and decrease the malondialdehyde content. The present results suggest that SHL possesses anti-inflammatory and antioxidative properties that may protect mice against LPS-induced ALI.

No MeSH data available.


Related in: MedlinePlus

Effects of SHL on the activities of SOD and CAT and the content of GSH and MDA in lung tissues. Mice received SHL or TEM once daily for 3 days. Lung homogenates were prepared 6 h after LPS treatment. Activities of SOD (a) and CAT (b) and the content of MDA (c) and GSH (d) were determined. Data represent means ± SD (n = 10). #P < 0.05 and ##P < 0.01 versus control group; *P < 0.05 and **P < 0.01 versus LPS group.
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fig7: Effects of SHL on the activities of SOD and CAT and the content of GSH and MDA in lung tissues. Mice received SHL or TEM once daily for 3 days. Lung homogenates were prepared 6 h after LPS treatment. Activities of SOD (a) and CAT (b) and the content of MDA (c) and GSH (d) were determined. Data represent means ± SD (n = 10). #P < 0.05 and ##P < 0.01 versus control group; *P < 0.05 and **P < 0.01 versus LPS group.

Mentions: Oxidative stress plays an important role in the development of LPS-induced ALI. To evaluate the effects of SHL on oxidative stress, the activities of SOD and CAT as well as the contents of GSH and MDA in the lungs were determined. As shown in Figures 7(a)–7(c), LPS markedly reduced the SOD (P < 0.01) and CAT activities (P < 0.05) and induced an overproduction of MDA (P < 0.05). SHL (5 and 10 mL/kg) or TEM could decrease the MDA content (P < 0.05), and 10 mL/kg SHL increased the SOD and CAT activities (P < 0.05).


Shuang-huang-lian attenuates lipopolysaccharide-induced acute lung injury in mice involving anti-inflammatory and antioxidative activities.

Fang L, Gao Y, Liu F, Hou R, Cai RL, Qi Y - Evid Based Complement Alternat Med (2015)

Effects of SHL on the activities of SOD and CAT and the content of GSH and MDA in lung tissues. Mice received SHL or TEM once daily for 3 days. Lung homogenates were prepared 6 h after LPS treatment. Activities of SOD (a) and CAT (b) and the content of MDA (c) and GSH (d) were determined. Data represent means ± SD (n = 10). #P < 0.05 and ##P < 0.01 versus control group; *P < 0.05 and **P < 0.01 versus LPS group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4402504&req=5

fig7: Effects of SHL on the activities of SOD and CAT and the content of GSH and MDA in lung tissues. Mice received SHL or TEM once daily for 3 days. Lung homogenates were prepared 6 h after LPS treatment. Activities of SOD (a) and CAT (b) and the content of MDA (c) and GSH (d) were determined. Data represent means ± SD (n = 10). #P < 0.05 and ##P < 0.01 versus control group; *P < 0.05 and **P < 0.01 versus LPS group.
Mentions: Oxidative stress plays an important role in the development of LPS-induced ALI. To evaluate the effects of SHL on oxidative stress, the activities of SOD and CAT as well as the contents of GSH and MDA in the lungs were determined. As shown in Figures 7(a)–7(c), LPS markedly reduced the SOD (P < 0.01) and CAT activities (P < 0.05) and induced an overproduction of MDA (P < 0.05). SHL (5 and 10 mL/kg) or TEM could decrease the MDA content (P < 0.05), and 10 mL/kg SHL increased the SOD and CAT activities (P < 0.05).

Bottom Line: Histological studies demonstrated that SHL attenuated LPS-induced interstitial edema, hemorrhage, and the infiltration of neutrophils into the lung tissue.Moreover, SHL could also enhance the superoxide dismutase and catalase activities, increase the reduced glutathione content, and decrease the malondialdehyde content.The present results suggest that SHL possesses anti-inflammatory and antioxidative properties that may protect mice against LPS-induced ALI.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.

ABSTRACT
Shuang-Huang-Lian (SHL) is a common traditional Chinese preparation extracted from Lonicerae Japonicae Flos, Scutellariae Radix, and Fructus Forsythiae. In this study, we demonstrate the anti-inflammatory and antioxidative effects of SHL on lipopolysaccharide- (LPS-) induced acute lung injury (ALI) in mice. SHL reduced the lung wet/dry weight ratio, lowered the number of total cells in the bronchoalveolar lavage fluid, and decreased the myeloperoxidase activity in lung tissues 6 h after LPS treatment. It also inhibited the overproduction of proinflammatory cytokines (TNF-α, IL-1β, and IL-6) in the bronchoalveolar lavage fluid. Histological studies demonstrated that SHL attenuated LPS-induced interstitial edema, hemorrhage, and the infiltration of neutrophils into the lung tissue. Moreover, SHL could also enhance the superoxide dismutase and catalase activities, increase the reduced glutathione content, and decrease the malondialdehyde content. The present results suggest that SHL possesses anti-inflammatory and antioxidative properties that may protect mice against LPS-induced ALI.

No MeSH data available.


Related in: MedlinePlus