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Shuang-huang-lian attenuates lipopolysaccharide-induced acute lung injury in mice involving anti-inflammatory and antioxidative activities.

Fang L, Gao Y, Liu F, Hou R, Cai RL, Qi Y - Evid Based Complement Alternat Med (2015)

Bottom Line: Histological studies demonstrated that SHL attenuated LPS-induced interstitial edema, hemorrhage, and the infiltration of neutrophils into the lung tissue.Moreover, SHL could also enhance the superoxide dismutase and catalase activities, increase the reduced glutathione content, and decrease the malondialdehyde content.The present results suggest that SHL possesses anti-inflammatory and antioxidative properties that may protect mice against LPS-induced ALI.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.

ABSTRACT
Shuang-Huang-Lian (SHL) is a common traditional Chinese preparation extracted from Lonicerae Japonicae Flos, Scutellariae Radix, and Fructus Forsythiae. In this study, we demonstrate the anti-inflammatory and antioxidative effects of SHL on lipopolysaccharide- (LPS-) induced acute lung injury (ALI) in mice. SHL reduced the lung wet/dry weight ratio, lowered the number of total cells in the bronchoalveolar lavage fluid, and decreased the myeloperoxidase activity in lung tissues 6 h after LPS treatment. It also inhibited the overproduction of proinflammatory cytokines (TNF-α, IL-1β, and IL-6) in the bronchoalveolar lavage fluid. Histological studies demonstrated that SHL attenuated LPS-induced interstitial edema, hemorrhage, and the infiltration of neutrophils into the lung tissue. Moreover, SHL could also enhance the superoxide dismutase and catalase activities, increase the reduced glutathione content, and decrease the malondialdehyde content. The present results suggest that SHL possesses anti-inflammatory and antioxidative properties that may protect mice against LPS-induced ALI.

No MeSH data available.


Related in: MedlinePlus

Effect of SHL on production of inflammatory cytokines (TNF-α, IL-1β, and IL-6) in BALF of LPS-induced ALI mice. Mice received (i.p.) SHL once daily for 3 days or DXM 30 min before LPS administration. BALF was collected 6 h after LPS administration to analyze TNF-α (a), IL-6 (b), and IL-1β (c) production. Data present the means ± SD (n = 10). ##P < 0.01 versus control group; **P < 0.01 versus LPS group.
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fig4: Effect of SHL on production of inflammatory cytokines (TNF-α, IL-1β, and IL-6) in BALF of LPS-induced ALI mice. Mice received (i.p.) SHL once daily for 3 days or DXM 30 min before LPS administration. BALF was collected 6 h after LPS administration to analyze TNF-α (a), IL-6 (b), and IL-1β (c) production. Data present the means ± SD (n = 10). ##P < 0.01 versus control group; **P < 0.01 versus LPS group.

Mentions: The effect of SHL on proinflammatory cytokines production was examined. As shown in Figure 4, exposure to LPS resulted in a significant increase (P < 0.01) in TNF-α, IL-1β, and IL-6 production compared with the control group. Pretreatment with SHL or DXM efficiently inhibited the TNF-α, IL-1β, and IL-6 production in a dose-dependent manner (P < 0.01).


Shuang-huang-lian attenuates lipopolysaccharide-induced acute lung injury in mice involving anti-inflammatory and antioxidative activities.

Fang L, Gao Y, Liu F, Hou R, Cai RL, Qi Y - Evid Based Complement Alternat Med (2015)

Effect of SHL on production of inflammatory cytokines (TNF-α, IL-1β, and IL-6) in BALF of LPS-induced ALI mice. Mice received (i.p.) SHL once daily for 3 days or DXM 30 min before LPS administration. BALF was collected 6 h after LPS administration to analyze TNF-α (a), IL-6 (b), and IL-1β (c) production. Data present the means ± SD (n = 10). ##P < 0.01 versus control group; **P < 0.01 versus LPS group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4402504&req=5

fig4: Effect of SHL on production of inflammatory cytokines (TNF-α, IL-1β, and IL-6) in BALF of LPS-induced ALI mice. Mice received (i.p.) SHL once daily for 3 days or DXM 30 min before LPS administration. BALF was collected 6 h after LPS administration to analyze TNF-α (a), IL-6 (b), and IL-1β (c) production. Data present the means ± SD (n = 10). ##P < 0.01 versus control group; **P < 0.01 versus LPS group.
Mentions: The effect of SHL on proinflammatory cytokines production was examined. As shown in Figure 4, exposure to LPS resulted in a significant increase (P < 0.01) in TNF-α, IL-1β, and IL-6 production compared with the control group. Pretreatment with SHL or DXM efficiently inhibited the TNF-α, IL-1β, and IL-6 production in a dose-dependent manner (P < 0.01).

Bottom Line: Histological studies demonstrated that SHL attenuated LPS-induced interstitial edema, hemorrhage, and the infiltration of neutrophils into the lung tissue.Moreover, SHL could also enhance the superoxide dismutase and catalase activities, increase the reduced glutathione content, and decrease the malondialdehyde content.The present results suggest that SHL possesses anti-inflammatory and antioxidative properties that may protect mice against LPS-induced ALI.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.

ABSTRACT
Shuang-Huang-Lian (SHL) is a common traditional Chinese preparation extracted from Lonicerae Japonicae Flos, Scutellariae Radix, and Fructus Forsythiae. In this study, we demonstrate the anti-inflammatory and antioxidative effects of SHL on lipopolysaccharide- (LPS-) induced acute lung injury (ALI) in mice. SHL reduced the lung wet/dry weight ratio, lowered the number of total cells in the bronchoalveolar lavage fluid, and decreased the myeloperoxidase activity in lung tissues 6 h after LPS treatment. It also inhibited the overproduction of proinflammatory cytokines (TNF-α, IL-1β, and IL-6) in the bronchoalveolar lavage fluid. Histological studies demonstrated that SHL attenuated LPS-induced interstitial edema, hemorrhage, and the infiltration of neutrophils into the lung tissue. Moreover, SHL could also enhance the superoxide dismutase and catalase activities, increase the reduced glutathione content, and decrease the malondialdehyde content. The present results suggest that SHL possesses anti-inflammatory and antioxidative properties that may protect mice against LPS-induced ALI.

No MeSH data available.


Related in: MedlinePlus