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Shuang-huang-lian attenuates lipopolysaccharide-induced acute lung injury in mice involving anti-inflammatory and antioxidative activities.

Fang L, Gao Y, Liu F, Hou R, Cai RL, Qi Y - Evid Based Complement Alternat Med (2015)

Bottom Line: Histological studies demonstrated that SHL attenuated LPS-induced interstitial edema, hemorrhage, and the infiltration of neutrophils into the lung tissue.Moreover, SHL could also enhance the superoxide dismutase and catalase activities, increase the reduced glutathione content, and decrease the malondialdehyde content.The present results suggest that SHL possesses anti-inflammatory and antioxidative properties that may protect mice against LPS-induced ALI.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.

ABSTRACT
Shuang-Huang-Lian (SHL) is a common traditional Chinese preparation extracted from Lonicerae Japonicae Flos, Scutellariae Radix, and Fructus Forsythiae. In this study, we demonstrate the anti-inflammatory and antioxidative effects of SHL on lipopolysaccharide- (LPS-) induced acute lung injury (ALI) in mice. SHL reduced the lung wet/dry weight ratio, lowered the number of total cells in the bronchoalveolar lavage fluid, and decreased the myeloperoxidase activity in lung tissues 6 h after LPS treatment. It also inhibited the overproduction of proinflammatory cytokines (TNF-α, IL-1β, and IL-6) in the bronchoalveolar lavage fluid. Histological studies demonstrated that SHL attenuated LPS-induced interstitial edema, hemorrhage, and the infiltration of neutrophils into the lung tissue. Moreover, SHL could also enhance the superoxide dismutase and catalase activities, increase the reduced glutathione content, and decrease the malondialdehyde content. The present results suggest that SHL possesses anti-inflammatory and antioxidative properties that may protect mice against LPS-induced ALI.

No MeSH data available.


Related in: MedlinePlus

Effect of SHL on histopathological change in lung tissues (200x, H&E staining). Mice received (i.p.) SHL (5 and 10 mL/kg) once daily for 3 days or DXM (5 mg/kg) 30 min before LPS administration. Lungs (n = 3) were processed to assess histological changes 6 h after LPS challenge. The arrows point to prominent neutrophil infiltration.
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fig3: Effect of SHL on histopathological change in lung tissues (200x, H&E staining). Mice received (i.p.) SHL (5 and 10 mL/kg) once daily for 3 days or DXM (5 mg/kg) 30 min before LPS administration. Lungs (n = 3) were processed to assess histological changes 6 h after LPS challenge. The arrows point to prominent neutrophil infiltration.

Mentions: A normal pulmonary structure was observed in the control mice (Figure 3(a)). In contrast, LPS-treated animals exhibited a moderate inflammatory reaction in the lung characterized by interstitial edema, alveolar hemorrhage, and prominent neutrophil infiltration into the interstitium and alveolar spaces (Figure 3(b)). Mice treated with SHL (10 mL/kg) or DXM showed a minimal inflammatory reaction manifested by minor interstitial edema and fewer neutrophils that invaded into the interstitium and alveolar spaces (Figures 3(c)–3(e)).


Shuang-huang-lian attenuates lipopolysaccharide-induced acute lung injury in mice involving anti-inflammatory and antioxidative activities.

Fang L, Gao Y, Liu F, Hou R, Cai RL, Qi Y - Evid Based Complement Alternat Med (2015)

Effect of SHL on histopathological change in lung tissues (200x, H&E staining). Mice received (i.p.) SHL (5 and 10 mL/kg) once daily for 3 days or DXM (5 mg/kg) 30 min before LPS administration. Lungs (n = 3) were processed to assess histological changes 6 h after LPS challenge. The arrows point to prominent neutrophil infiltration.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4402504&req=5

fig3: Effect of SHL on histopathological change in lung tissues (200x, H&E staining). Mice received (i.p.) SHL (5 and 10 mL/kg) once daily for 3 days or DXM (5 mg/kg) 30 min before LPS administration. Lungs (n = 3) were processed to assess histological changes 6 h after LPS challenge. The arrows point to prominent neutrophil infiltration.
Mentions: A normal pulmonary structure was observed in the control mice (Figure 3(a)). In contrast, LPS-treated animals exhibited a moderate inflammatory reaction in the lung characterized by interstitial edema, alveolar hemorrhage, and prominent neutrophil infiltration into the interstitium and alveolar spaces (Figure 3(b)). Mice treated with SHL (10 mL/kg) or DXM showed a minimal inflammatory reaction manifested by minor interstitial edema and fewer neutrophils that invaded into the interstitium and alveolar spaces (Figures 3(c)–3(e)).

Bottom Line: Histological studies demonstrated that SHL attenuated LPS-induced interstitial edema, hemorrhage, and the infiltration of neutrophils into the lung tissue.Moreover, SHL could also enhance the superoxide dismutase and catalase activities, increase the reduced glutathione content, and decrease the malondialdehyde content.The present results suggest that SHL possesses anti-inflammatory and antioxidative properties that may protect mice against LPS-induced ALI.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.

ABSTRACT
Shuang-Huang-Lian (SHL) is a common traditional Chinese preparation extracted from Lonicerae Japonicae Flos, Scutellariae Radix, and Fructus Forsythiae. In this study, we demonstrate the anti-inflammatory and antioxidative effects of SHL on lipopolysaccharide- (LPS-) induced acute lung injury (ALI) in mice. SHL reduced the lung wet/dry weight ratio, lowered the number of total cells in the bronchoalveolar lavage fluid, and decreased the myeloperoxidase activity in lung tissues 6 h after LPS treatment. It also inhibited the overproduction of proinflammatory cytokines (TNF-α, IL-1β, and IL-6) in the bronchoalveolar lavage fluid. Histological studies demonstrated that SHL attenuated LPS-induced interstitial edema, hemorrhage, and the infiltration of neutrophils into the lung tissue. Moreover, SHL could also enhance the superoxide dismutase and catalase activities, increase the reduced glutathione content, and decrease the malondialdehyde content. The present results suggest that SHL possesses anti-inflammatory and antioxidative properties that may protect mice against LPS-induced ALI.

No MeSH data available.


Related in: MedlinePlus