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Shuang-huang-lian attenuates lipopolysaccharide-induced acute lung injury in mice involving anti-inflammatory and antioxidative activities.

Fang L, Gao Y, Liu F, Hou R, Cai RL, Qi Y - Evid Based Complement Alternat Med (2015)

Bottom Line: Histological studies demonstrated that SHL attenuated LPS-induced interstitial edema, hemorrhage, and the infiltration of neutrophils into the lung tissue.Moreover, SHL could also enhance the superoxide dismutase and catalase activities, increase the reduced glutathione content, and decrease the malondialdehyde content.The present results suggest that SHL possesses anti-inflammatory and antioxidative properties that may protect mice against LPS-induced ALI.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.

ABSTRACT
Shuang-Huang-Lian (SHL) is a common traditional Chinese preparation extracted from Lonicerae Japonicae Flos, Scutellariae Radix, and Fructus Forsythiae. In this study, we demonstrate the anti-inflammatory and antioxidative effects of SHL on lipopolysaccharide- (LPS-) induced acute lung injury (ALI) in mice. SHL reduced the lung wet/dry weight ratio, lowered the number of total cells in the bronchoalveolar lavage fluid, and decreased the myeloperoxidase activity in lung tissues 6 h after LPS treatment. It also inhibited the overproduction of proinflammatory cytokines (TNF-α, IL-1β, and IL-6) in the bronchoalveolar lavage fluid. Histological studies demonstrated that SHL attenuated LPS-induced interstitial edema, hemorrhage, and the infiltration of neutrophils into the lung tissue. Moreover, SHL could also enhance the superoxide dismutase and catalase activities, increase the reduced glutathione content, and decrease the malondialdehyde content. The present results suggest that SHL possesses anti-inflammatory and antioxidative properties that may protect mice against LPS-induced ALI.

No MeSH data available.


Related in: MedlinePlus

Schedule of pretreatment with SHL and positive drugs and mice induced by LPS.
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Related In: Results  -  Collection


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fig1: Schedule of pretreatment with SHL and positive drugs and mice induced by LPS.

Mentions: Balb/c mice (male, 18–20 g) were purchased from Vital River Experimental Animal Services (Beijing, China) and housed in standard laboratory temperature and humidity conditions with a 12 h light/dark cycle. They were randomly divided into five groups: normal control group (NC), model group (LPS), SHL groups (5 and 10 mL/kg), and positive control group (DXM or TEM). Because glucocorticoids have widely been used as anti-inflammatory drugs in the clinical treatment of ALI and TEM has also been shown to act as a SOD mimetic to significantly protect from LPS-induced ALI [10], we selected DXM and TEM as positive controls to evaluate the anti-inflammatory and antioxidative effects of SHL. The pretreatment with SHL and positive drugs as a function of time is shown in Figure 1; SHL or TEM was administered intraperitoneally (i.p.) once per day for 3 consecutive days, while DXM was administered once 30 min prior to LPS administration. Mice in the control and model groups received an equal volume of physiological saline. The mice were anesthetized 30 min after the last administration. Subsequently, LPS (2 μg per mouse) was intratracheally instilled [11]. The mice in the control group received an equal volume of physiological saline without LPS.


Shuang-huang-lian attenuates lipopolysaccharide-induced acute lung injury in mice involving anti-inflammatory and antioxidative activities.

Fang L, Gao Y, Liu F, Hou R, Cai RL, Qi Y - Evid Based Complement Alternat Med (2015)

Schedule of pretreatment with SHL and positive drugs and mice induced by LPS.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4402504&req=5

fig1: Schedule of pretreatment with SHL and positive drugs and mice induced by LPS.
Mentions: Balb/c mice (male, 18–20 g) were purchased from Vital River Experimental Animal Services (Beijing, China) and housed in standard laboratory temperature and humidity conditions with a 12 h light/dark cycle. They were randomly divided into five groups: normal control group (NC), model group (LPS), SHL groups (5 and 10 mL/kg), and positive control group (DXM or TEM). Because glucocorticoids have widely been used as anti-inflammatory drugs in the clinical treatment of ALI and TEM has also been shown to act as a SOD mimetic to significantly protect from LPS-induced ALI [10], we selected DXM and TEM as positive controls to evaluate the anti-inflammatory and antioxidative effects of SHL. The pretreatment with SHL and positive drugs as a function of time is shown in Figure 1; SHL or TEM was administered intraperitoneally (i.p.) once per day for 3 consecutive days, while DXM was administered once 30 min prior to LPS administration. Mice in the control and model groups received an equal volume of physiological saline. The mice were anesthetized 30 min after the last administration. Subsequently, LPS (2 μg per mouse) was intratracheally instilled [11]. The mice in the control group received an equal volume of physiological saline without LPS.

Bottom Line: Histological studies demonstrated that SHL attenuated LPS-induced interstitial edema, hemorrhage, and the infiltration of neutrophils into the lung tissue.Moreover, SHL could also enhance the superoxide dismutase and catalase activities, increase the reduced glutathione content, and decrease the malondialdehyde content.The present results suggest that SHL possesses anti-inflammatory and antioxidative properties that may protect mice against LPS-induced ALI.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.

ABSTRACT
Shuang-Huang-Lian (SHL) is a common traditional Chinese preparation extracted from Lonicerae Japonicae Flos, Scutellariae Radix, and Fructus Forsythiae. In this study, we demonstrate the anti-inflammatory and antioxidative effects of SHL on lipopolysaccharide- (LPS-) induced acute lung injury (ALI) in mice. SHL reduced the lung wet/dry weight ratio, lowered the number of total cells in the bronchoalveolar lavage fluid, and decreased the myeloperoxidase activity in lung tissues 6 h after LPS treatment. It also inhibited the overproduction of proinflammatory cytokines (TNF-α, IL-1β, and IL-6) in the bronchoalveolar lavage fluid. Histological studies demonstrated that SHL attenuated LPS-induced interstitial edema, hemorrhage, and the infiltration of neutrophils into the lung tissue. Moreover, SHL could also enhance the superoxide dismutase and catalase activities, increase the reduced glutathione content, and decrease the malondialdehyde content. The present results suggest that SHL possesses anti-inflammatory and antioxidative properties that may protect mice against LPS-induced ALI.

No MeSH data available.


Related in: MedlinePlus