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Analysis of the in vivo and in vitro effects of photodynamic therapy on breast cancer by using a sensitizer, sinoporphyrin sodium.

Wang X, Hu J, Wang P, Zhang S, Liu Y, Xiong W, Liu Q - Theranostics (2015)

Bottom Line: In addition, DVDMS-PDT effectively inhibited the migration of 4T1 cells in scratch wound-healing and transwell assays.Moreover, preliminary toxicological results indicate that DVDMS is relatively safe.These results suggest that DVDMS is a promising sensitizer that warrants further development for use in cancer treatment with PDT or other sensitizing agent-based therapies.

View Article: PubMed Central - PubMed

Affiliation: 1. Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, Ministry of Education, National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi, People's Republic of China.

ABSTRACT
Photodynamic therapy (PDT) is an emerging theranostic modality for various cancers and diseases. Photosensitizers are critical components for PDT. Sinoporphyrin sodium, referred to as DVDMS, is a newly identified photosensitizer that was isolated from Photofrin. Here, we evaluated the effects of DVDMS-mediated PDT (DVDMS-PDT) on tumor cell proliferation and metastasis in the highly metastatic 4T1 cell line and a mouse xenograft model. DVDMS-PDT elicited a potent phototoxic effect in vitro, which was abolished using the reactive oxygen species (ROS) scavenger N-acetylcysteine. In addition, DVDMS-PDT effectively inhibited the migration of 4T1 cells in scratch wound-healing and transwell assays. Using an in vivo mouse model, DVDMS-PDT greatly prolonged the survival time of tumor-bearing mice and inhibited tumor growth and lung metastasis, consistent with in vitro findings. PDT with DVDMS had a greater anti-tumor efficacy than clinically used Photofrin. Moreover, preliminary toxicological results indicate that DVDMS is relatively safe. These results suggest that DVDMS is a promising sensitizer that warrants further development for use in cancer treatment with PDT or other sensitizing agent-based therapies.

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DVDMS-PDT inhibits tumor growth (A-C) and prolonged the survival time of 4T1 bearing mice (D). A: 4T1-bearing mice were treated with 50 J/cm2 light and different DVDMS dose (0.5, 1, 2 mg/kg). B: 4T1-bearing mice were treated by 2 mg/kg DVDMS combined with different light dose (50, 100, 150 J/cm2). C: 4T1-bearing mice were treated by 2 mg/kg DVDMS or 10 mg/kg PF with 100 J/cm2 light. Error bars represent the SD from three independent experiments. * p < 0.05, ** p < 0.01 versus control, # p < 0.05, ## p < 0.01 versus 2 mg/kg DVDMS alone group, △△p < 0.01 2 mg/kg DVDMS + 100 J/cm2 light group versus 10 mg/kg PF+ 100 J/cm2 light group. D: Survival curves of 4T1-bearing mice in different groups. * p < 0.05 10 mg/kg PF+ 100 J/cm2 light group versus control, ** p < 0.01 2 mg/kg DVDMS + 50 J/cm2 light group versus control and DVDMS alone, # p < 0.05 2 mg/kg DVDMS + 50 J/cm2 light group versus 10 mg/kg PF + 100 J/cm2 light group.
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Figure 7: DVDMS-PDT inhibits tumor growth (A-C) and prolonged the survival time of 4T1 bearing mice (D). A: 4T1-bearing mice were treated with 50 J/cm2 light and different DVDMS dose (0.5, 1, 2 mg/kg). B: 4T1-bearing mice were treated by 2 mg/kg DVDMS combined with different light dose (50, 100, 150 J/cm2). C: 4T1-bearing mice were treated by 2 mg/kg DVDMS or 10 mg/kg PF with 100 J/cm2 light. Error bars represent the SD from three independent experiments. * p < 0.05, ** p < 0.01 versus control, # p < 0.05, ## p < 0.01 versus 2 mg/kg DVDMS alone group, △△p < 0.01 2 mg/kg DVDMS + 100 J/cm2 light group versus 10 mg/kg PF+ 100 J/cm2 light group. D: Survival curves of 4T1-bearing mice in different groups. * p < 0.05 10 mg/kg PF+ 100 J/cm2 light group versus control, ** p < 0.01 2 mg/kg DVDMS + 50 J/cm2 light group versus control and DVDMS alone, # p < 0.05 2 mg/kg DVDMS + 50 J/cm2 light group versus 10 mg/kg PF + 100 J/cm2 light group.

Mentions: To investigate the in vivo anti-cancer efficacy of DVDMS-PDT, a 4T1 mouse mammary cancer model was utilized. The anti-cancer efficacy of DVDMS-PDT was compared with that of PF-PDT. DVDMS-PDT significantly prolonged the survival of the 4T1 mammary cancer-bearing mice compared to control and DVDMS alone groups (Fig. 7D, p < 0.01). DVDMS-PDT was also more effective than PF-PDT at increasing survival of tumor-bearing mice (p < 0.05).


Analysis of the in vivo and in vitro effects of photodynamic therapy on breast cancer by using a sensitizer, sinoporphyrin sodium.

Wang X, Hu J, Wang P, Zhang S, Liu Y, Xiong W, Liu Q - Theranostics (2015)

DVDMS-PDT inhibits tumor growth (A-C) and prolonged the survival time of 4T1 bearing mice (D). A: 4T1-bearing mice were treated with 50 J/cm2 light and different DVDMS dose (0.5, 1, 2 mg/kg). B: 4T1-bearing mice were treated by 2 mg/kg DVDMS combined with different light dose (50, 100, 150 J/cm2). C: 4T1-bearing mice were treated by 2 mg/kg DVDMS or 10 mg/kg PF with 100 J/cm2 light. Error bars represent the SD from three independent experiments. * p < 0.05, ** p < 0.01 versus control, # p < 0.05, ## p < 0.01 versus 2 mg/kg DVDMS alone group, △△p < 0.01 2 mg/kg DVDMS + 100 J/cm2 light group versus 10 mg/kg PF+ 100 J/cm2 light group. D: Survival curves of 4T1-bearing mice in different groups. * p < 0.05 10 mg/kg PF+ 100 J/cm2 light group versus control, ** p < 0.01 2 mg/kg DVDMS + 50 J/cm2 light group versus control and DVDMS alone, # p < 0.05 2 mg/kg DVDMS + 50 J/cm2 light group versus 10 mg/kg PF + 100 J/cm2 light group.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4402500&req=5

Figure 7: DVDMS-PDT inhibits tumor growth (A-C) and prolonged the survival time of 4T1 bearing mice (D). A: 4T1-bearing mice were treated with 50 J/cm2 light and different DVDMS dose (0.5, 1, 2 mg/kg). B: 4T1-bearing mice were treated by 2 mg/kg DVDMS combined with different light dose (50, 100, 150 J/cm2). C: 4T1-bearing mice were treated by 2 mg/kg DVDMS or 10 mg/kg PF with 100 J/cm2 light. Error bars represent the SD from three independent experiments. * p < 0.05, ** p < 0.01 versus control, # p < 0.05, ## p < 0.01 versus 2 mg/kg DVDMS alone group, △△p < 0.01 2 mg/kg DVDMS + 100 J/cm2 light group versus 10 mg/kg PF+ 100 J/cm2 light group. D: Survival curves of 4T1-bearing mice in different groups. * p < 0.05 10 mg/kg PF+ 100 J/cm2 light group versus control, ** p < 0.01 2 mg/kg DVDMS + 50 J/cm2 light group versus control and DVDMS alone, # p < 0.05 2 mg/kg DVDMS + 50 J/cm2 light group versus 10 mg/kg PF + 100 J/cm2 light group.
Mentions: To investigate the in vivo anti-cancer efficacy of DVDMS-PDT, a 4T1 mouse mammary cancer model was utilized. The anti-cancer efficacy of DVDMS-PDT was compared with that of PF-PDT. DVDMS-PDT significantly prolonged the survival of the 4T1 mammary cancer-bearing mice compared to control and DVDMS alone groups (Fig. 7D, p < 0.01). DVDMS-PDT was also more effective than PF-PDT at increasing survival of tumor-bearing mice (p < 0.05).

Bottom Line: In addition, DVDMS-PDT effectively inhibited the migration of 4T1 cells in scratch wound-healing and transwell assays.Moreover, preliminary toxicological results indicate that DVDMS is relatively safe.These results suggest that DVDMS is a promising sensitizer that warrants further development for use in cancer treatment with PDT or other sensitizing agent-based therapies.

View Article: PubMed Central - PubMed

Affiliation: 1. Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, Ministry of Education, National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi, People's Republic of China.

ABSTRACT
Photodynamic therapy (PDT) is an emerging theranostic modality for various cancers and diseases. Photosensitizers are critical components for PDT. Sinoporphyrin sodium, referred to as DVDMS, is a newly identified photosensitizer that was isolated from Photofrin. Here, we evaluated the effects of DVDMS-mediated PDT (DVDMS-PDT) on tumor cell proliferation and metastasis in the highly metastatic 4T1 cell line and a mouse xenograft model. DVDMS-PDT elicited a potent phototoxic effect in vitro, which was abolished using the reactive oxygen species (ROS) scavenger N-acetylcysteine. In addition, DVDMS-PDT effectively inhibited the migration of 4T1 cells in scratch wound-healing and transwell assays. Using an in vivo mouse model, DVDMS-PDT greatly prolonged the survival time of tumor-bearing mice and inhibited tumor growth and lung metastasis, consistent with in vitro findings. PDT with DVDMS had a greater anti-tumor efficacy than clinically used Photofrin. Moreover, preliminary toxicological results indicate that DVDMS is relatively safe. These results suggest that DVDMS is a promising sensitizer that warrants further development for use in cancer treatment with PDT or other sensitizing agent-based therapies.

Show MeSH
Related in: MedlinePlus