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Analysis of the in vivo and in vitro effects of photodynamic therapy on breast cancer by using a sensitizer, sinoporphyrin sodium.

Wang X, Hu J, Wang P, Zhang S, Liu Y, Xiong W, Liu Q - Theranostics (2015)

Bottom Line: In addition, DVDMS-PDT effectively inhibited the migration of 4T1 cells in scratch wound-healing and transwell assays.Moreover, preliminary toxicological results indicate that DVDMS is relatively safe.These results suggest that DVDMS is a promising sensitizer that warrants further development for use in cancer treatment with PDT or other sensitizing agent-based therapies.

View Article: PubMed Central - PubMed

Affiliation: 1. Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, Ministry of Education, National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi, People's Republic of China.

ABSTRACT
Photodynamic therapy (PDT) is an emerging theranostic modality for various cancers and diseases. Photosensitizers are critical components for PDT. Sinoporphyrin sodium, referred to as DVDMS, is a newly identified photosensitizer that was isolated from Photofrin. Here, we evaluated the effects of DVDMS-mediated PDT (DVDMS-PDT) on tumor cell proliferation and metastasis in the highly metastatic 4T1 cell line and a mouse xenograft model. DVDMS-PDT elicited a potent phototoxic effect in vitro, which was abolished using the reactive oxygen species (ROS) scavenger N-acetylcysteine. In addition, DVDMS-PDT effectively inhibited the migration of 4T1 cells in scratch wound-healing and transwell assays. Using an in vivo mouse model, DVDMS-PDT greatly prolonged the survival time of tumor-bearing mice and inhibited tumor growth and lung metastasis, consistent with in vitro findings. PDT with DVDMS had a greater anti-tumor efficacy than clinically used Photofrin. Moreover, preliminary toxicological results indicate that DVDMS is relatively safe. These results suggest that DVDMS is a promising sensitizer that warrants further development for use in cancer treatment with PDT or other sensitizing agent-based therapies.

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Related in: MedlinePlus

DVDMS-PDT inhibits the cell motility of 4T1 cells. A: The effects of the indicated treatments on cell migration using a scratch wound-healing assay are shown. B: Analyses of cell migration using a transwell assay. Error bars represent the SD from three independent experiments. * p < 0.05, ** p < 0.01 versus control, ## p < 0.01 versus DVDMS alone, &&p < 0.01 PF-PDT group versus DVDMS-PDT group. C: Scanning electron micrographs of 4T1 cells at 24 h after different the indicated treatments are shown. D: Images of the F-actin cytoskeleton in 4T1 cells after the indicated treatments are shown. The representative phenomenon is denoted by white arrows. FITC-phalloidin (green) and HO stained nuclei (blue).
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Figure 5: DVDMS-PDT inhibits the cell motility of 4T1 cells. A: The effects of the indicated treatments on cell migration using a scratch wound-healing assay are shown. B: Analyses of cell migration using a transwell assay. Error bars represent the SD from three independent experiments. * p < 0.05, ** p < 0.01 versus control, ## p < 0.01 versus DVDMS alone, &&p < 0.01 PF-PDT group versus DVDMS-PDT group. C: Scanning electron micrographs of 4T1 cells at 24 h after different the indicated treatments are shown. D: Images of the F-actin cytoskeleton in 4T1 cells after the indicated treatments are shown. The representative phenomenon is denoted by white arrows. FITC-phalloidin (green) and HO stained nuclei (blue).

Mentions: Cell migration was assessed using scratch wound-healing and transwell assays. In the scratch wound-healing assay, cells in the untreated and DVDMS alone groups filled the wound completely after 24 h, while the DVDMS-PDT groups exhibited reduced migration into the wound in a dose-dependent manner (Fig. 5A). The PF-PDT group also exhibited a decrease in migration; however, the effect was less than that for the DVDMS-PDT group at the same light dose.


Analysis of the in vivo and in vitro effects of photodynamic therapy on breast cancer by using a sensitizer, sinoporphyrin sodium.

Wang X, Hu J, Wang P, Zhang S, Liu Y, Xiong W, Liu Q - Theranostics (2015)

DVDMS-PDT inhibits the cell motility of 4T1 cells. A: The effects of the indicated treatments on cell migration using a scratch wound-healing assay are shown. B: Analyses of cell migration using a transwell assay. Error bars represent the SD from three independent experiments. * p < 0.05, ** p < 0.01 versus control, ## p < 0.01 versus DVDMS alone, &&p < 0.01 PF-PDT group versus DVDMS-PDT group. C: Scanning electron micrographs of 4T1 cells at 24 h after different the indicated treatments are shown. D: Images of the F-actin cytoskeleton in 4T1 cells after the indicated treatments are shown. The representative phenomenon is denoted by white arrows. FITC-phalloidin (green) and HO stained nuclei (blue).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4402500&req=5

Figure 5: DVDMS-PDT inhibits the cell motility of 4T1 cells. A: The effects of the indicated treatments on cell migration using a scratch wound-healing assay are shown. B: Analyses of cell migration using a transwell assay. Error bars represent the SD from three independent experiments. * p < 0.05, ** p < 0.01 versus control, ## p < 0.01 versus DVDMS alone, &&p < 0.01 PF-PDT group versus DVDMS-PDT group. C: Scanning electron micrographs of 4T1 cells at 24 h after different the indicated treatments are shown. D: Images of the F-actin cytoskeleton in 4T1 cells after the indicated treatments are shown. The representative phenomenon is denoted by white arrows. FITC-phalloidin (green) and HO stained nuclei (blue).
Mentions: Cell migration was assessed using scratch wound-healing and transwell assays. In the scratch wound-healing assay, cells in the untreated and DVDMS alone groups filled the wound completely after 24 h, while the DVDMS-PDT groups exhibited reduced migration into the wound in a dose-dependent manner (Fig. 5A). The PF-PDT group also exhibited a decrease in migration; however, the effect was less than that for the DVDMS-PDT group at the same light dose.

Bottom Line: In addition, DVDMS-PDT effectively inhibited the migration of 4T1 cells in scratch wound-healing and transwell assays.Moreover, preliminary toxicological results indicate that DVDMS is relatively safe.These results suggest that DVDMS is a promising sensitizer that warrants further development for use in cancer treatment with PDT or other sensitizing agent-based therapies.

View Article: PubMed Central - PubMed

Affiliation: 1. Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, Ministry of Education, National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi, People's Republic of China.

ABSTRACT
Photodynamic therapy (PDT) is an emerging theranostic modality for various cancers and diseases. Photosensitizers are critical components for PDT. Sinoporphyrin sodium, referred to as DVDMS, is a newly identified photosensitizer that was isolated from Photofrin. Here, we evaluated the effects of DVDMS-mediated PDT (DVDMS-PDT) on tumor cell proliferation and metastasis in the highly metastatic 4T1 cell line and a mouse xenograft model. DVDMS-PDT elicited a potent phototoxic effect in vitro, which was abolished using the reactive oxygen species (ROS) scavenger N-acetylcysteine. In addition, DVDMS-PDT effectively inhibited the migration of 4T1 cells in scratch wound-healing and transwell assays. Using an in vivo mouse model, DVDMS-PDT greatly prolonged the survival time of tumor-bearing mice and inhibited tumor growth and lung metastasis, consistent with in vitro findings. PDT with DVDMS had a greater anti-tumor efficacy than clinically used Photofrin. Moreover, preliminary toxicological results indicate that DVDMS is relatively safe. These results suggest that DVDMS is a promising sensitizer that warrants further development for use in cancer treatment with PDT or other sensitizing agent-based therapies.

Show MeSH
Related in: MedlinePlus