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Regulation of Osteoblast Differentiation by Acid-Etched and/or Grit-Blasted Titanium Substrate Topography Is Enhanced by 1,25(OH)2D3 in a Sex-Dependent Manner.

Olivares-Navarrete R, Hyzy SL, Boyan BD, Schwartz Z - Biomed Res Int (2015)

Bottom Line: Primary osteoblasts were also sensitive to SLA, with less effect from individual structural components, demonstrated by enhanced local factor production.The effect of 1α,25(OH)2D3 was sex dependent.The results also suggest that levels of 1α,25(OH)2D3 in the patient may be important in osseointegration.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, School of Engineering, Virginia Commonwealth University, 601 W. Main Street, Richmond, VA 23284, USA.

ABSTRACT
This study assessed contributions of micron-scale topography on clinically relevant titanium (Ti) to differentiation of osteoprogenitor cells and osteoblasts; the interaction of this effect with 1α,25-dihydroxyvitamin D3 (1α,25(OH)2D3); and if the effects are sex-dependent. Male and female rat bone marrow cells (BMCs) were cultured on acid-etched (A, R a = 0.87 μm), grit-blasted (GB, R a = 3.90 μm), or grit-blasted/acid-etched (SLA, R a = 3.22 μm) Ti. BMCs were sensitive to surface topography and underwent osteoblast differentiation. This was greatest on SLA; acid etching and grit blasting contributed additively. Primary osteoblasts were also sensitive to SLA, with less effect from individual structural components, demonstrated by enhanced local factor production. Sex-dependent responses of BMCs to topography varied with parameter whereas male and female osteoblasts responded similarly to surface treatment. 1α,25(OH)2D3 enhanced cell responses on all surfaces similarly. Effects were sex-dependent and male cells grown on a complex microstructured surface were much more sensitive than female cells. These results indicate that effects of the complex SLA topography are greater than acid etching or grit blasting alone on multipotent BMCs and committed osteoblasts and that individual parameters are sex-specific. The effect of 1α,25(OH)2D3 was sex dependent. The results also suggest that levels of 1α,25(OH)2D3 in the patient may be important in osseointegration.

No MeSH data available.


Effect of Ti surface topography with or without 1α,25(OH)2D3 on osteoblast differentiation of rat calvarial osteoblasts. Male and female calvarial osteoblasts were cultured on TCPS or Ti disks. At confluence, cells were treated for 24 hours with 10−8 M 1α,25(OH)2D3. OPG (a), active TGF-β1 (b), and latent TGF-β1 (c) were measured in the conditioned media. Data are displayed as treatment/control of cells on Ti surfaces to cells on TCPS. *P < 0.05 versus TCPS; $P < 0.05 versus A surface; &P < 0.05 versus GB; •P < 0.05, female versus male; #P < 0.05 versus 0 M 1α,25(OH)2D3.
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fig5: Effect of Ti surface topography with or without 1α,25(OH)2D3 on osteoblast differentiation of rat calvarial osteoblasts. Male and female calvarial osteoblasts were cultured on TCPS or Ti disks. At confluence, cells were treated for 24 hours with 10−8 M 1α,25(OH)2D3. OPG (a), active TGF-β1 (b), and latent TGF-β1 (c) were measured in the conditioned media. Data are displayed as treatment/control of cells on Ti surfaces to cells on TCPS. *P < 0.05 versus TCPS; $P < 0.05 versus A surface; &P < 0.05 versus GB; •P < 0.05, female versus male; #P < 0.05 versus 0 M 1α,25(OH)2D3.

Mentions: OPG was also increased in osteoblast cultures on Ti surfaces in comparison to TCPS (SLA > A > GB) (Figure 5(a)). This was even more evident in female osteoblast cultures. 1α,25(OH)2D3 increased OPG by more than 100% in osteoblasts on all surfaces (A, SLA > GB) with no difference between male and female. The response of female osteoblasts was less robust on A and SLA but was comparable to the male cells on GB. Active TGF-β1 was increased in male and female osteoblasts on all surfaces in comparison to control (SLA < A < GB) (Figure 5(b)). Treatment with 1α,25(OH)2D3 caused significant increases in TGF-β1 in male and female cells on all surfaces (GB < A < SLA). In the untreated osteoblasts, there was no difference between male and female cells in TGF-β1 levels. However, treatment with 1α,25(OH)2D3 caused a statistically greater increase in male cells than in female cells on A and GB surfaces; however, there was no difference on SLA substrates. Latent TGF-β1 was increased in male osteoblasts on all surfaces in comparison to control and in female osteoblasts grown on A and SLA surfaces; female osteoblasts on GB surfaces were not significantly different from control (Figure 5(c)). Treatment with 1α,25(OH)2D3 increased latent TGF-β1 in male and female cells on all surfaces following a similar pattern (GB < A < SLA). Male cells had significantly higher levels of latent TGF-β1 than females on all surfaces after treatment with 1α,25(OH)2D3; untreated female osteoblasts had lower levels of TGF-β1 on GB surfaces than male cells.


Regulation of Osteoblast Differentiation by Acid-Etched and/or Grit-Blasted Titanium Substrate Topography Is Enhanced by 1,25(OH)2D3 in a Sex-Dependent Manner.

Olivares-Navarrete R, Hyzy SL, Boyan BD, Schwartz Z - Biomed Res Int (2015)

Effect of Ti surface topography with or without 1α,25(OH)2D3 on osteoblast differentiation of rat calvarial osteoblasts. Male and female calvarial osteoblasts were cultured on TCPS or Ti disks. At confluence, cells were treated for 24 hours with 10−8 M 1α,25(OH)2D3. OPG (a), active TGF-β1 (b), and latent TGF-β1 (c) were measured in the conditioned media. Data are displayed as treatment/control of cells on Ti surfaces to cells on TCPS. *P < 0.05 versus TCPS; $P < 0.05 versus A surface; &P < 0.05 versus GB; •P < 0.05, female versus male; #P < 0.05 versus 0 M 1α,25(OH)2D3.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4402479&req=5

fig5: Effect of Ti surface topography with or without 1α,25(OH)2D3 on osteoblast differentiation of rat calvarial osteoblasts. Male and female calvarial osteoblasts were cultured on TCPS or Ti disks. At confluence, cells were treated for 24 hours with 10−8 M 1α,25(OH)2D3. OPG (a), active TGF-β1 (b), and latent TGF-β1 (c) were measured in the conditioned media. Data are displayed as treatment/control of cells on Ti surfaces to cells on TCPS. *P < 0.05 versus TCPS; $P < 0.05 versus A surface; &P < 0.05 versus GB; •P < 0.05, female versus male; #P < 0.05 versus 0 M 1α,25(OH)2D3.
Mentions: OPG was also increased in osteoblast cultures on Ti surfaces in comparison to TCPS (SLA > A > GB) (Figure 5(a)). This was even more evident in female osteoblast cultures. 1α,25(OH)2D3 increased OPG by more than 100% in osteoblasts on all surfaces (A, SLA > GB) with no difference between male and female. The response of female osteoblasts was less robust on A and SLA but was comparable to the male cells on GB. Active TGF-β1 was increased in male and female osteoblasts on all surfaces in comparison to control (SLA < A < GB) (Figure 5(b)). Treatment with 1α,25(OH)2D3 caused significant increases in TGF-β1 in male and female cells on all surfaces (GB < A < SLA). In the untreated osteoblasts, there was no difference between male and female cells in TGF-β1 levels. However, treatment with 1α,25(OH)2D3 caused a statistically greater increase in male cells than in female cells on A and GB surfaces; however, there was no difference on SLA substrates. Latent TGF-β1 was increased in male osteoblasts on all surfaces in comparison to control and in female osteoblasts grown on A and SLA surfaces; female osteoblasts on GB surfaces were not significantly different from control (Figure 5(c)). Treatment with 1α,25(OH)2D3 increased latent TGF-β1 in male and female cells on all surfaces following a similar pattern (GB < A < SLA). Male cells had significantly higher levels of latent TGF-β1 than females on all surfaces after treatment with 1α,25(OH)2D3; untreated female osteoblasts had lower levels of TGF-β1 on GB surfaces than male cells.

Bottom Line: Primary osteoblasts were also sensitive to SLA, with less effect from individual structural components, demonstrated by enhanced local factor production.The effect of 1α,25(OH)2D3 was sex dependent.The results also suggest that levels of 1α,25(OH)2D3 in the patient may be important in osseointegration.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, School of Engineering, Virginia Commonwealth University, 601 W. Main Street, Richmond, VA 23284, USA.

ABSTRACT
This study assessed contributions of micron-scale topography on clinically relevant titanium (Ti) to differentiation of osteoprogenitor cells and osteoblasts; the interaction of this effect with 1α,25-dihydroxyvitamin D3 (1α,25(OH)2D3); and if the effects are sex-dependent. Male and female rat bone marrow cells (BMCs) were cultured on acid-etched (A, R a = 0.87 μm), grit-blasted (GB, R a = 3.90 μm), or grit-blasted/acid-etched (SLA, R a = 3.22 μm) Ti. BMCs were sensitive to surface topography and underwent osteoblast differentiation. This was greatest on SLA; acid etching and grit blasting contributed additively. Primary osteoblasts were also sensitive to SLA, with less effect from individual structural components, demonstrated by enhanced local factor production. Sex-dependent responses of BMCs to topography varied with parameter whereas male and female osteoblasts responded similarly to surface treatment. 1α,25(OH)2D3 enhanced cell responses on all surfaces similarly. Effects were sex-dependent and male cells grown on a complex microstructured surface were much more sensitive than female cells. These results indicate that effects of the complex SLA topography are greater than acid etching or grit blasting alone on multipotent BMCs and committed osteoblasts and that individual parameters are sex-specific. The effect of 1α,25(OH)2D3 was sex dependent. The results also suggest that levels of 1α,25(OH)2D3 in the patient may be important in osseointegration.

No MeSH data available.