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Regulation of Osteoblast Differentiation by Acid-Etched and/or Grit-Blasted Titanium Substrate Topography Is Enhanced by 1,25(OH)2D3 in a Sex-Dependent Manner.

Olivares-Navarrete R, Hyzy SL, Boyan BD, Schwartz Z - Biomed Res Int (2015)

Bottom Line: Primary osteoblasts were also sensitive to SLA, with less effect from individual structural components, demonstrated by enhanced local factor production.The effect of 1α,25(OH)2D3 was sex dependent.The results also suggest that levels of 1α,25(OH)2D3 in the patient may be important in osseointegration.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, School of Engineering, Virginia Commonwealth University, 601 W. Main Street, Richmond, VA 23284, USA.

ABSTRACT
This study assessed contributions of micron-scale topography on clinically relevant titanium (Ti) to differentiation of osteoprogenitor cells and osteoblasts; the interaction of this effect with 1α,25-dihydroxyvitamin D3 (1α,25(OH)2D3); and if the effects are sex-dependent. Male and female rat bone marrow cells (BMCs) were cultured on acid-etched (A, R a = 0.87 μm), grit-blasted (GB, R a = 3.90 μm), or grit-blasted/acid-etched (SLA, R a = 3.22 μm) Ti. BMCs were sensitive to surface topography and underwent osteoblast differentiation. This was greatest on SLA; acid etching and grit blasting contributed additively. Primary osteoblasts were also sensitive to SLA, with less effect from individual structural components, demonstrated by enhanced local factor production. Sex-dependent responses of BMCs to topography varied with parameter whereas male and female osteoblasts responded similarly to surface treatment. 1α,25(OH)2D3 enhanced cell responses on all surfaces similarly. Effects were sex-dependent and male cells grown on a complex microstructured surface were much more sensitive than female cells. These results indicate that effects of the complex SLA topography are greater than acid etching or grit blasting alone on multipotent BMCs and committed osteoblasts and that individual parameters are sex-specific. The effect of 1α,25(OH)2D3 was sex dependent. The results also suggest that levels of 1α,25(OH)2D3 in the patient may be important in osseointegration.

No MeSH data available.


Response of rat BMCs to microstructured titanium surfaces. Male and female BMCs were cultured on TCPS or Ti disks and grown to confluence. VEGF was measured in the conditioned media. Data are displayed as treatment/control of cells on Ti surfaces to cells on TCPS. *P < 0.05 versus TCPS; $P < 0.05 versus A surface; &P < 0.05 versus GB; •P < 0.05, female versus male.
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fig3: Response of rat BMCs to microstructured titanium surfaces. Male and female BMCs were cultured on TCPS or Ti disks and grown to confluence. VEGF was measured in the conditioned media. Data are displayed as treatment/control of cells on Ti surfaces to cells on TCPS. *P < 0.05 versus TCPS; $P < 0.05 versus A surface; &P < 0.05 versus GB; •P < 0.05, female versus male.

Mentions: VEGF was also produced in a surface-dependent, sex-specific manner by BMC cells (Figure 3). Male cells produced more VEGF on GB and SLA than on TCPS. In contrast, female cells produced more VEGF on A and SLA than on GB. Female cells produced more VEGF-A on acid-etched A and SLA surfaces than male cells.


Regulation of Osteoblast Differentiation by Acid-Etched and/or Grit-Blasted Titanium Substrate Topography Is Enhanced by 1,25(OH)2D3 in a Sex-Dependent Manner.

Olivares-Navarrete R, Hyzy SL, Boyan BD, Schwartz Z - Biomed Res Int (2015)

Response of rat BMCs to microstructured titanium surfaces. Male and female BMCs were cultured on TCPS or Ti disks and grown to confluence. VEGF was measured in the conditioned media. Data are displayed as treatment/control of cells on Ti surfaces to cells on TCPS. *P < 0.05 versus TCPS; $P < 0.05 versus A surface; &P < 0.05 versus GB; •P < 0.05, female versus male.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4402479&req=5

fig3: Response of rat BMCs to microstructured titanium surfaces. Male and female BMCs were cultured on TCPS or Ti disks and grown to confluence. VEGF was measured in the conditioned media. Data are displayed as treatment/control of cells on Ti surfaces to cells on TCPS. *P < 0.05 versus TCPS; $P < 0.05 versus A surface; &P < 0.05 versus GB; •P < 0.05, female versus male.
Mentions: VEGF was also produced in a surface-dependent, sex-specific manner by BMC cells (Figure 3). Male cells produced more VEGF on GB and SLA than on TCPS. In contrast, female cells produced more VEGF on A and SLA than on GB. Female cells produced more VEGF-A on acid-etched A and SLA surfaces than male cells.

Bottom Line: Primary osteoblasts were also sensitive to SLA, with less effect from individual structural components, demonstrated by enhanced local factor production.The effect of 1α,25(OH)2D3 was sex dependent.The results also suggest that levels of 1α,25(OH)2D3 in the patient may be important in osseointegration.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, School of Engineering, Virginia Commonwealth University, 601 W. Main Street, Richmond, VA 23284, USA.

ABSTRACT
This study assessed contributions of micron-scale topography on clinically relevant titanium (Ti) to differentiation of osteoprogenitor cells and osteoblasts; the interaction of this effect with 1α,25-dihydroxyvitamin D3 (1α,25(OH)2D3); and if the effects are sex-dependent. Male and female rat bone marrow cells (BMCs) were cultured on acid-etched (A, R a = 0.87 μm), grit-blasted (GB, R a = 3.90 μm), or grit-blasted/acid-etched (SLA, R a = 3.22 μm) Ti. BMCs were sensitive to surface topography and underwent osteoblast differentiation. This was greatest on SLA; acid etching and grit blasting contributed additively. Primary osteoblasts were also sensitive to SLA, with less effect from individual structural components, demonstrated by enhanced local factor production. Sex-dependent responses of BMCs to topography varied with parameter whereas male and female osteoblasts responded similarly to surface treatment. 1α,25(OH)2D3 enhanced cell responses on all surfaces similarly. Effects were sex-dependent and male cells grown on a complex microstructured surface were much more sensitive than female cells. These results indicate that effects of the complex SLA topography are greater than acid etching or grit blasting alone on multipotent BMCs and committed osteoblasts and that individual parameters are sex-specific. The effect of 1α,25(OH)2D3 was sex dependent. The results also suggest that levels of 1α,25(OH)2D3 in the patient may be important in osseointegration.

No MeSH data available.