Limits...
Regulation of Osteoblast Differentiation by Acid-Etched and/or Grit-Blasted Titanium Substrate Topography Is Enhanced by 1,25(OH)2D3 in a Sex-Dependent Manner.

Olivares-Navarrete R, Hyzy SL, Boyan BD, Schwartz Z - Biomed Res Int (2015)

Bottom Line: Primary osteoblasts were also sensitive to SLA, with less effect from individual structural components, demonstrated by enhanced local factor production.The effect of 1α,25(OH)2D3 was sex dependent.The results also suggest that levels of 1α,25(OH)2D3 in the patient may be important in osseointegration.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, School of Engineering, Virginia Commonwealth University, 601 W. Main Street, Richmond, VA 23284, USA.

ABSTRACT
This study assessed contributions of micron-scale topography on clinically relevant titanium (Ti) to differentiation of osteoprogenitor cells and osteoblasts; the interaction of this effect with 1α,25-dihydroxyvitamin D3 (1α,25(OH)2D3); and if the effects are sex-dependent. Male and female rat bone marrow cells (BMCs) were cultured on acid-etched (A, R a = 0.87 μm), grit-blasted (GB, R a = 3.90 μm), or grit-blasted/acid-etched (SLA, R a = 3.22 μm) Ti. BMCs were sensitive to surface topography and underwent osteoblast differentiation. This was greatest on SLA; acid etching and grit blasting contributed additively. Primary osteoblasts were also sensitive to SLA, with less effect from individual structural components, demonstrated by enhanced local factor production. Sex-dependent responses of BMCs to topography varied with parameter whereas male and female osteoblasts responded similarly to surface treatment. 1α,25(OH)2D3 enhanced cell responses on all surfaces similarly. Effects were sex-dependent and male cells grown on a complex microstructured surface were much more sensitive than female cells. These results indicate that effects of the complex SLA topography are greater than acid etching or grit blasting alone on multipotent BMCs and committed osteoblasts and that individual parameters are sex-specific. The effect of 1α,25(OH)2D3 was sex dependent. The results also suggest that levels of 1α,25(OH)2D3 in the patient may be important in osseointegration.

No MeSH data available.


Response of rat BMCs to microstructured titanium surfaces. Male and female BMCs were cultured on TCPS, A, GB, or SLA surfaces and grown to confluence. Cell number (a), alkaline phosphatase specific activity (b), and osteocalcin levels (c) were measured. Data are displayed as treatment/control of cells on Ti surfaces to cells on TCPS. *P < 0.05 versus TCPS; $P < 0.05 versus A surface; &P < 0.05 versus GB; •P < 0.05, female versus male.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4402479&req=5

fig1: Response of rat BMCs to microstructured titanium surfaces. Male and female BMCs were cultured on TCPS, A, GB, or SLA surfaces and grown to confluence. Cell number (a), alkaline phosphatase specific activity (b), and osteocalcin levels (c) were measured. Data are displayed as treatment/control of cells on Ti surfaces to cells on TCPS. *P < 0.05 versus TCPS; $P < 0.05 versus A surface; &P < 0.05 versus GB; •P < 0.05, female versus male.

Mentions: BMCs had lower cell number when cultured on Ti substrates in comparison to TCPS (Figure 1(a)). This effect was significantly lower on SLA compared to GB and A and significantly lower on A than GB. Female cells had higher cell number on GB and lower cell number on SLA than male cells. Alkaline phosphatase specific activity was sensitive to surface topography in a sex-specific manner (Figure 1(b)). Activity was increased in male cells on A and SLA in comparison to TCPS. However, in female BMC cultures, activity was increased only on in cells on GB. Both male and female BMCs exhibited increased osteocalcin in their conditioned media on all surfaces (SLA > A > GB) (Figure 1(c)). This effect was comparable in male and female cultures on GB surfaces, but cells isolated from female rats produced more osteocalcin on SLA than males.


Regulation of Osteoblast Differentiation by Acid-Etched and/or Grit-Blasted Titanium Substrate Topography Is Enhanced by 1,25(OH)2D3 in a Sex-Dependent Manner.

Olivares-Navarrete R, Hyzy SL, Boyan BD, Schwartz Z - Biomed Res Int (2015)

Response of rat BMCs to microstructured titanium surfaces. Male and female BMCs were cultured on TCPS, A, GB, or SLA surfaces and grown to confluence. Cell number (a), alkaline phosphatase specific activity (b), and osteocalcin levels (c) were measured. Data are displayed as treatment/control of cells on Ti surfaces to cells on TCPS. *P < 0.05 versus TCPS; $P < 0.05 versus A surface; &P < 0.05 versus GB; •P < 0.05, female versus male.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4402479&req=5

fig1: Response of rat BMCs to microstructured titanium surfaces. Male and female BMCs were cultured on TCPS, A, GB, or SLA surfaces and grown to confluence. Cell number (a), alkaline phosphatase specific activity (b), and osteocalcin levels (c) were measured. Data are displayed as treatment/control of cells on Ti surfaces to cells on TCPS. *P < 0.05 versus TCPS; $P < 0.05 versus A surface; &P < 0.05 versus GB; •P < 0.05, female versus male.
Mentions: BMCs had lower cell number when cultured on Ti substrates in comparison to TCPS (Figure 1(a)). This effect was significantly lower on SLA compared to GB and A and significantly lower on A than GB. Female cells had higher cell number on GB and lower cell number on SLA than male cells. Alkaline phosphatase specific activity was sensitive to surface topography in a sex-specific manner (Figure 1(b)). Activity was increased in male cells on A and SLA in comparison to TCPS. However, in female BMC cultures, activity was increased only on in cells on GB. Both male and female BMCs exhibited increased osteocalcin in their conditioned media on all surfaces (SLA > A > GB) (Figure 1(c)). This effect was comparable in male and female cultures on GB surfaces, but cells isolated from female rats produced more osteocalcin on SLA than males.

Bottom Line: Primary osteoblasts were also sensitive to SLA, with less effect from individual structural components, demonstrated by enhanced local factor production.The effect of 1α,25(OH)2D3 was sex dependent.The results also suggest that levels of 1α,25(OH)2D3 in the patient may be important in osseointegration.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, School of Engineering, Virginia Commonwealth University, 601 W. Main Street, Richmond, VA 23284, USA.

ABSTRACT
This study assessed contributions of micron-scale topography on clinically relevant titanium (Ti) to differentiation of osteoprogenitor cells and osteoblasts; the interaction of this effect with 1α,25-dihydroxyvitamin D3 (1α,25(OH)2D3); and if the effects are sex-dependent. Male and female rat bone marrow cells (BMCs) were cultured on acid-etched (A, R a = 0.87 μm), grit-blasted (GB, R a = 3.90 μm), or grit-blasted/acid-etched (SLA, R a = 3.22 μm) Ti. BMCs were sensitive to surface topography and underwent osteoblast differentiation. This was greatest on SLA; acid etching and grit blasting contributed additively. Primary osteoblasts were also sensitive to SLA, with less effect from individual structural components, demonstrated by enhanced local factor production. Sex-dependent responses of BMCs to topography varied with parameter whereas male and female osteoblasts responded similarly to surface treatment. 1α,25(OH)2D3 enhanced cell responses on all surfaces similarly. Effects were sex-dependent and male cells grown on a complex microstructured surface were much more sensitive than female cells. These results indicate that effects of the complex SLA topography are greater than acid etching or grit blasting alone on multipotent BMCs and committed osteoblasts and that individual parameters are sex-specific. The effect of 1α,25(OH)2D3 was sex dependent. The results also suggest that levels of 1α,25(OH)2D3 in the patient may be important in osseointegration.

No MeSH data available.