Limits...
Micronuclei versus Chromosomal Aberrations Induced by X-Ray in Radiosensitive Mammalian Cells.

Plamadeala C, Wojcik A, Creanga D - Iran. J. Public Health (2015)

Bottom Line: In vitro study was carried out on the genotoxicity of low-medium doses of 190 kV X-rays absorbed in Chinese hamster ovary cell cultures.Polynomial dose-response curves were evidenced for cells with Ni micronuclei (i=1,3) while non-monotonic curves were evidenced through detailed analysis of aberrant cells with Ni chromosomal changes [Formula: see text] - in concordance with in vitro studies from literature.The investigation could be important for public health issues where micronucleus screening is routinely applied but also for research purposes where various chromosomal aberrations could be of particular interest.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Physics, "Alexandru Ioan Cuza" University, Iasi, Romania.

ABSTRACT

Background: An experimental study was accomplished to compare estimation methods of ionizing radiations genotoxicity in mammalian cell cultures by means of two cytogenetic parameters with focus on aberrant cells characterized by multiple chromosomal damages.

Methods: In vitro study was carried out on the genotoxicity of low-medium doses of 190 kV X-rays absorbed in Chinese hamster ovary cell cultures. Micronuclei and ten types of chromosomal aberrations were identified with Giemsa dying and optical microscope screening.

Results: The first parameter consisting in micronuclei relative frequency has led to higher linear correlation coefficient than the second one consistent with chromosomal aberrations relative frequency. However, the latter parameter estimated as the sum of all chromosomal aberrations appeared to be more sensitive to radiation dose increasing in the studied dose range, from 0 to 3 Gy. The number of micronuclei occurring simultaneously in a single cell was not higher than 3, while the number of chromosomal aberrations observed in the same cell reached the value of 5 for doses over 1 Gy.

Conclusion: Polynomial dose-response curves were evidenced for cells with Ni micronuclei (i=1,3) while non-monotonic curves were evidenced through detailed analysis of aberrant cells with Ni chromosomal changes [Formula: see text] - in concordance with in vitro studies from literature. The investigation could be important for public health issues where micronucleus screening is routinely applied but also for research purposes where various chromosomal aberrations could be of particular interest.

No MeSH data available.


Related in: MedlinePlus

Dose-response curve for micronuclei
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4402410&req=5

Figure 1: Dose-response curve for micronuclei

Mentions: In Fig. 1 the micronuclei relative frequency (MN), calculated with relation [1] was given as function of the radiation dose:[1]MN=1×N1+2×N2+3×N3N,where N1, N2 and N3 are the numbers of cells presenting 1; 2; respectively 3 micronuclei while N is the total number of analyzed cells: N = N0+N1+N2+N3, with N0 representing the number of cells missing micronuclei.


Micronuclei versus Chromosomal Aberrations Induced by X-Ray in Radiosensitive Mammalian Cells.

Plamadeala C, Wojcik A, Creanga D - Iran. J. Public Health (2015)

Dose-response curve for micronuclei
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4402410&req=5

Figure 1: Dose-response curve for micronuclei
Mentions: In Fig. 1 the micronuclei relative frequency (MN), calculated with relation [1] was given as function of the radiation dose:[1]MN=1×N1+2×N2+3×N3N,where N1, N2 and N3 are the numbers of cells presenting 1; 2; respectively 3 micronuclei while N is the total number of analyzed cells: N = N0+N1+N2+N3, with N0 representing the number of cells missing micronuclei.

Bottom Line: In vitro study was carried out on the genotoxicity of low-medium doses of 190 kV X-rays absorbed in Chinese hamster ovary cell cultures.Polynomial dose-response curves were evidenced for cells with Ni micronuclei (i=1,3) while non-monotonic curves were evidenced through detailed analysis of aberrant cells with Ni chromosomal changes [Formula: see text] - in concordance with in vitro studies from literature.The investigation could be important for public health issues where micronucleus screening is routinely applied but also for research purposes where various chromosomal aberrations could be of particular interest.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Physics, "Alexandru Ioan Cuza" University, Iasi, Romania.

ABSTRACT

Background: An experimental study was accomplished to compare estimation methods of ionizing radiations genotoxicity in mammalian cell cultures by means of two cytogenetic parameters with focus on aberrant cells characterized by multiple chromosomal damages.

Methods: In vitro study was carried out on the genotoxicity of low-medium doses of 190 kV X-rays absorbed in Chinese hamster ovary cell cultures. Micronuclei and ten types of chromosomal aberrations were identified with Giemsa dying and optical microscope screening.

Results: The first parameter consisting in micronuclei relative frequency has led to higher linear correlation coefficient than the second one consistent with chromosomal aberrations relative frequency. However, the latter parameter estimated as the sum of all chromosomal aberrations appeared to be more sensitive to radiation dose increasing in the studied dose range, from 0 to 3 Gy. The number of micronuclei occurring simultaneously in a single cell was not higher than 3, while the number of chromosomal aberrations observed in the same cell reached the value of 5 for doses over 1 Gy.

Conclusion: Polynomial dose-response curves were evidenced for cells with Ni micronuclei (i=1,3) while non-monotonic curves were evidenced through detailed analysis of aberrant cells with Ni chromosomal changes [Formula: see text] - in concordance with in vitro studies from literature. The investigation could be important for public health issues where micronucleus screening is routinely applied but also for research purposes where various chromosomal aberrations could be of particular interest.

No MeSH data available.


Related in: MedlinePlus