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Pharmacological and Genotoxic Properties of Polyphenolic Extracts of Cedrela odorata L. and Juglans regia L. Barks in Rodents.

Almonte-Flores DC, Paniagua-Castro N, Escalona-Cardoso G, Rosales-Castro M - Evid Based Complement Alternat Med (2015)

Bottom Line: Juglans regia showed greater extract concentration and higher antioxidant activity.Polyphenolic extracts reduced cholesterol levels in a hypercholesterolemic mouse model and decreased hepatic lipid peroxidation.Genotoxic effects were evaluated by the comet assay, which showed that while J. regia extract had no effect, C. odorata extract induced slight damage at 200 mg/kg, with the formation of type 0 and 1 comets.

View Article: PubMed Central - PubMed

Affiliation: CIIDIR-IPN Unidad Durango, Sigma 119 Fraccionamiento 20 de Noviembre, 34220 Durango, DGO, Mexico.

ABSTRACT
Evaluation of the phenolic compounds and antioxidant activity of Cedrela odorata L. and Juglans regia L. bark extracts was performed in vitro. Juglans regia showed greater extract concentration and higher antioxidant activity. Hypoglycemic activity in rats was assessed by generating a glucose tolerance curve and determining the area under the curve (AUC). Diabetes was later induced by an injection with streptozotocin (65 mg/kg of b.w.) and confirmed after 24 hours. The extract was administered (200 mg/kg b.w.) over 10 days, and blood glucose was monitored and compared with a control group. The glucose AUC showed a hypoglycemic effect of J. regia and C. odorata in normal rats. Both extracts reduced hepatic lipid peroxidation in diabetic rats. Polyphenolic extracts reduced cholesterol levels in a hypercholesterolemic mouse model and decreased hepatic lipid peroxidation. Polyphenolic extract doses of 100 and 200 mg/kg b.w. were administered alone or with cyclophosphamide (CPA) 50 mg/kg ip, which was used as a positive control. Analyses were performed using leukocytes in a comet assay after 4 and 24 h of treatment. Genotoxic effects were evaluated by the comet assay, which showed that while J. regia extract had no effect, C. odorata extract induced slight damage at 200 mg/kg, with the formation of type 0 and 1 comets.

No MeSH data available.


Related in: MedlinePlus

Glucose tolerance test in rats. (a) Glucose levels. (b) Area under the glucose curve. Two-way RM ANOVA for (a) and one-way ANOVA for (b).  ∗P < 0.05, significant difference compared with the control group.
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fig1: Glucose tolerance test in rats. (a) Glucose levels. (b) Area under the glucose curve. Two-way RM ANOVA for (a) and one-way ANOVA for (b).  ∗P < 0.05, significant difference compared with the control group.

Mentions: In regard to the glucose tolerance test in rats, C. odorata and J. regia extracts significantly decreased blood glucose levels (94.3 ± 17.2 g/L min and 88.8 ± 5.8 g/L min, resp.) (Figure 1) compared with the control group. Extracts of J. regia showed the highest hypoglycemic activity (P < 0.05).


Pharmacological and Genotoxic Properties of Polyphenolic Extracts of Cedrela odorata L. and Juglans regia L. Barks in Rodents.

Almonte-Flores DC, Paniagua-Castro N, Escalona-Cardoso G, Rosales-Castro M - Evid Based Complement Alternat Med (2015)

Glucose tolerance test in rats. (a) Glucose levels. (b) Area under the glucose curve. Two-way RM ANOVA for (a) and one-way ANOVA for (b).  ∗P < 0.05, significant difference compared with the control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4402197&req=5

fig1: Glucose tolerance test in rats. (a) Glucose levels. (b) Area under the glucose curve. Two-way RM ANOVA for (a) and one-way ANOVA for (b).  ∗P < 0.05, significant difference compared with the control group.
Mentions: In regard to the glucose tolerance test in rats, C. odorata and J. regia extracts significantly decreased blood glucose levels (94.3 ± 17.2 g/L min and 88.8 ± 5.8 g/L min, resp.) (Figure 1) compared with the control group. Extracts of J. regia showed the highest hypoglycemic activity (P < 0.05).

Bottom Line: Juglans regia showed greater extract concentration and higher antioxidant activity.Polyphenolic extracts reduced cholesterol levels in a hypercholesterolemic mouse model and decreased hepatic lipid peroxidation.Genotoxic effects were evaluated by the comet assay, which showed that while J. regia extract had no effect, C. odorata extract induced slight damage at 200 mg/kg, with the formation of type 0 and 1 comets.

View Article: PubMed Central - PubMed

Affiliation: CIIDIR-IPN Unidad Durango, Sigma 119 Fraccionamiento 20 de Noviembre, 34220 Durango, DGO, Mexico.

ABSTRACT
Evaluation of the phenolic compounds and antioxidant activity of Cedrela odorata L. and Juglans regia L. bark extracts was performed in vitro. Juglans regia showed greater extract concentration and higher antioxidant activity. Hypoglycemic activity in rats was assessed by generating a glucose tolerance curve and determining the area under the curve (AUC). Diabetes was later induced by an injection with streptozotocin (65 mg/kg of b.w.) and confirmed after 24 hours. The extract was administered (200 mg/kg b.w.) over 10 days, and blood glucose was monitored and compared with a control group. The glucose AUC showed a hypoglycemic effect of J. regia and C. odorata in normal rats. Both extracts reduced hepatic lipid peroxidation in diabetic rats. Polyphenolic extracts reduced cholesterol levels in a hypercholesterolemic mouse model and decreased hepatic lipid peroxidation. Polyphenolic extract doses of 100 and 200 mg/kg b.w. were administered alone or with cyclophosphamide (CPA) 50 mg/kg ip, which was used as a positive control. Analyses were performed using leukocytes in a comet assay after 4 and 24 h of treatment. Genotoxic effects were evaluated by the comet assay, which showed that while J. regia extract had no effect, C. odorata extract induced slight damage at 200 mg/kg, with the formation of type 0 and 1 comets.

No MeSH data available.


Related in: MedlinePlus