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Successful isolation of viable adipose-derived stem cells from human adipose tissue subject to long-term cryopreservation: positive implications for adult stem cell-based therapeutics in patients of advanced age.

Devitt SM, Carter CM, Dierov R, Weiss S, Gersch RP, Percec I - Stem Cells Int (2015)

Bottom Line: Significantly more viable cells were initially isolated from tissue cryopreserved <1 year than from tissue cryopreserved >2 years, irrespective of patient age.Mesenchymal stem cell markers were maintained in all cohorts tested throughout the duration of the study.Patient age does not significantly impact stem cell isolation, viability, or growth.

View Article: PubMed Central - PubMed

Affiliation: Thomas Jefferson University Hospital, 132 S 10th Street No. 763J, Philadelphia, PA 19107, USA.

ABSTRACT
We examined cell isolation, viability, and growth in adipose-derived stem cells harvested from whole adipose tissue subject to different cryopreservation lengths (2-1159 days) from patients of varying ages (26-62 years). Subcutaneous abdominal adipose tissue was excised during abdominoplasties and was cryopreserved. The viability and number of adipose-derived stem cells isolated were measured after initial isolation and after 9, 18, and 28 days of growth. Data were analyzed with respect to cryopreservation duration and patient age. Significantly more viable cells were initially isolated from tissue cryopreserved <1 year than from tissue cryopreserved >2 years, irrespective of patient age. However, this difference did not persist with continued growth and there were no significant differences in cell viability or growth at subsequent time points with respect to cryopreservation duration or patient age. Mesenchymal stem cell markers were maintained in all cohorts tested throughout the duration of the study. Consequently, longer cryopreservation negatively impacts initial live adipose-derived stem cell isolation; however, this effect is neutralized with continued cell growth. Patient age does not significantly impact stem cell isolation, viability, or growth. Cryopreservation of adipose tissue is an effective long-term banking method for isolation of adipose-derived stem cells in patients of varying ages.

No MeSH data available.


Initial ASC viability relative to patient age. (a) There was a modest trend toward increasing ASC viability with advancing patient age. (b) ASC viability was compared relative to patient age across 4 cohort groups: <40 years (N = 12) versus ≥40 years (N = 20); <50 years (N = 23) versus ≥50 years (N = 9); and <40 years (N = 12) versus ≥50 years (N = 9). No significant differences in ASC viability were observed between any of the age cohorts; P > 0.05.
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fig5: Initial ASC viability relative to patient age. (a) There was a modest trend toward increasing ASC viability with advancing patient age. (b) ASC viability was compared relative to patient age across 4 cohort groups: <40 years (N = 12) versus ≥40 years (N = 20); <50 years (N = 23) versus ≥50 years (N = 9); and <40 years (N = 12) versus ≥50 years (N = 9). No significant differences in ASC viability were observed between any of the age cohorts; P > 0.05.

Mentions: Similarly, no significant differences in initial ASC viability relative to patient age were observed between groups, although a modest increase with advancing patient age was noted (Figure 5). ASC viability was compared between the following age cohorts: <40 years versus ≥40 years (54.13 ± 35.61% and 68.73 ± 27.29%, resp.), <50 years versus ≥50 years (61.8 ± 31.85% and 66.94 ± 30.03%, resp.), and <40 years versus ≥50 years (54.13 ± 35.61% and 66.94 ± 30.03%, resp.). No significant differences in ASC viability were observed between any of these age cohorts, P > 0.05 (Figure 5).


Successful isolation of viable adipose-derived stem cells from human adipose tissue subject to long-term cryopreservation: positive implications for adult stem cell-based therapeutics in patients of advanced age.

Devitt SM, Carter CM, Dierov R, Weiss S, Gersch RP, Percec I - Stem Cells Int (2015)

Initial ASC viability relative to patient age. (a) There was a modest trend toward increasing ASC viability with advancing patient age. (b) ASC viability was compared relative to patient age across 4 cohort groups: <40 years (N = 12) versus ≥40 years (N = 20); <50 years (N = 23) versus ≥50 years (N = 9); and <40 years (N = 12) versus ≥50 years (N = 9). No significant differences in ASC viability were observed between any of the age cohorts; P > 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4402176&req=5

fig5: Initial ASC viability relative to patient age. (a) There was a modest trend toward increasing ASC viability with advancing patient age. (b) ASC viability was compared relative to patient age across 4 cohort groups: <40 years (N = 12) versus ≥40 years (N = 20); <50 years (N = 23) versus ≥50 years (N = 9); and <40 years (N = 12) versus ≥50 years (N = 9). No significant differences in ASC viability were observed between any of the age cohorts; P > 0.05.
Mentions: Similarly, no significant differences in initial ASC viability relative to patient age were observed between groups, although a modest increase with advancing patient age was noted (Figure 5). ASC viability was compared between the following age cohorts: <40 years versus ≥40 years (54.13 ± 35.61% and 68.73 ± 27.29%, resp.), <50 years versus ≥50 years (61.8 ± 31.85% and 66.94 ± 30.03%, resp.), and <40 years versus ≥50 years (54.13 ± 35.61% and 66.94 ± 30.03%, resp.). No significant differences in ASC viability were observed between any of these age cohorts, P > 0.05 (Figure 5).

Bottom Line: Significantly more viable cells were initially isolated from tissue cryopreserved <1 year than from tissue cryopreserved >2 years, irrespective of patient age.Mesenchymal stem cell markers were maintained in all cohorts tested throughout the duration of the study.Patient age does not significantly impact stem cell isolation, viability, or growth.

View Article: PubMed Central - PubMed

Affiliation: Thomas Jefferson University Hospital, 132 S 10th Street No. 763J, Philadelphia, PA 19107, USA.

ABSTRACT
We examined cell isolation, viability, and growth in adipose-derived stem cells harvested from whole adipose tissue subject to different cryopreservation lengths (2-1159 days) from patients of varying ages (26-62 years). Subcutaneous abdominal adipose tissue was excised during abdominoplasties and was cryopreserved. The viability and number of adipose-derived stem cells isolated were measured after initial isolation and after 9, 18, and 28 days of growth. Data were analyzed with respect to cryopreservation duration and patient age. Significantly more viable cells were initially isolated from tissue cryopreserved <1 year than from tissue cryopreserved >2 years, irrespective of patient age. However, this difference did not persist with continued growth and there were no significant differences in cell viability or growth at subsequent time points with respect to cryopreservation duration or patient age. Mesenchymal stem cell markers were maintained in all cohorts tested throughout the duration of the study. Consequently, longer cryopreservation negatively impacts initial live adipose-derived stem cell isolation; however, this effect is neutralized with continued cell growth. Patient age does not significantly impact stem cell isolation, viability, or growth. Cryopreservation of adipose tissue is an effective long-term banking method for isolation of adipose-derived stem cells in patients of varying ages.

No MeSH data available.