Limits...
Successful isolation of viable adipose-derived stem cells from human adipose tissue subject to long-term cryopreservation: positive implications for adult stem cell-based therapeutics in patients of advanced age.

Devitt SM, Carter CM, Dierov R, Weiss S, Gersch RP, Percec I - Stem Cells Int (2015)

Bottom Line: Significantly more viable cells were initially isolated from tissue cryopreserved <1 year than from tissue cryopreserved >2 years, irrespective of patient age.Mesenchymal stem cell markers were maintained in all cohorts tested throughout the duration of the study.Patient age does not significantly impact stem cell isolation, viability, or growth.

View Article: PubMed Central - PubMed

Affiliation: Thomas Jefferson University Hospital, 132 S 10th Street No. 763J, Philadelphia, PA 19107, USA.

ABSTRACT
We examined cell isolation, viability, and growth in adipose-derived stem cells harvested from whole adipose tissue subject to different cryopreservation lengths (2-1159 days) from patients of varying ages (26-62 years). Subcutaneous abdominal adipose tissue was excised during abdominoplasties and was cryopreserved. The viability and number of adipose-derived stem cells isolated were measured after initial isolation and after 9, 18, and 28 days of growth. Data were analyzed with respect to cryopreservation duration and patient age. Significantly more viable cells were initially isolated from tissue cryopreserved <1 year than from tissue cryopreserved >2 years, irrespective of patient age. However, this difference did not persist with continued growth and there were no significant differences in cell viability or growth at subsequent time points with respect to cryopreservation duration or patient age. Mesenchymal stem cell markers were maintained in all cohorts tested throughout the duration of the study. Consequently, longer cryopreservation negatively impacts initial live adipose-derived stem cell isolation; however, this effect is neutralized with continued cell growth. Patient age does not significantly impact stem cell isolation, viability, or growth. Cryopreservation of adipose tissue is an effective long-term banking method for isolation of adipose-derived stem cells in patients of varying ages.

No MeSH data available.


Live ASC isolation relative to patient age. (a) ASCs were isolated from 32 patients of different ages (range 26–62 years, average 43.2 ± 9.7). Live ASCs isolated ranged from 0 to 95.5 × 104 cells/g adipose, average 63.8 ± 30.6, with no clear correlation between ASC isolation and patient age. (b) Live cell count was compared relative to patient age across 4 cohort groups: <40 years (N = 12) versus ≥40 years (N = 20); <50 years (N = 23) versus ≥50 years (N = 9); and <40 years (N = 12) versus ≥50 years (N = 9). No significant differences in live ASC isolation were observed between any of the age cohorts; P > 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4402176&req=5

fig4: Live ASC isolation relative to patient age. (a) ASCs were isolated from 32 patients of different ages (range 26–62 years, average 43.2 ± 9.7). Live ASCs isolated ranged from 0 to 95.5 × 104 cells/g adipose, average 63.8 ± 30.6, with no clear correlation between ASC isolation and patient age. (b) Live cell count was compared relative to patient age across 4 cohort groups: <40 years (N = 12) versus ≥40 years (N = 20); <50 years (N = 23) versus ≥50 years (N = 9); and <40 years (N = 12) versus ≥50 years (N = 9). No significant differences in live ASC isolation were observed between any of the age cohorts; P > 0.05.

Mentions: Live ASCs isolated ranged from 0 to 5.9 × 104 cells/g tissue, averaging 2.95 × 104 ± 2.5 × 104 cells/g tissue with no clear correlation between ASC isolation and patient age (Figure 4). The initial live cells were counted for each frozen tissue sample and compared between the following age cohort pairs: <40 years versus ≥40 years (2.65 × 104 ± 1.8 × 104 cells/g tissue and 2.6 × 104 ± 1.65 × 104 cells/g tissue, resp.), <50 years versus ≥50 years (2.44 × 104 ± 1.48 × 104 cell/g tissue and 3.07 × 104 ± 2.14 × 104 cells/g tissue, resp.), and <40 years versus ≥50 years (2.65 × 104 ± 1.8 × 104 cells/g tissue and 3.07 × 104 ± 2.14 × 104 cells/g tissue, resp.). No significant differences in live ASC isolation were observed between any of the age cohorts, P > 0.05 (Figure 4).


Successful isolation of viable adipose-derived stem cells from human adipose tissue subject to long-term cryopreservation: positive implications for adult stem cell-based therapeutics in patients of advanced age.

Devitt SM, Carter CM, Dierov R, Weiss S, Gersch RP, Percec I - Stem Cells Int (2015)

Live ASC isolation relative to patient age. (a) ASCs were isolated from 32 patients of different ages (range 26–62 years, average 43.2 ± 9.7). Live ASCs isolated ranged from 0 to 95.5 × 104 cells/g adipose, average 63.8 ± 30.6, with no clear correlation between ASC isolation and patient age. (b) Live cell count was compared relative to patient age across 4 cohort groups: <40 years (N = 12) versus ≥40 years (N = 20); <50 years (N = 23) versus ≥50 years (N = 9); and <40 years (N = 12) versus ≥50 years (N = 9). No significant differences in live ASC isolation were observed between any of the age cohorts; P > 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4402176&req=5

fig4: Live ASC isolation relative to patient age. (a) ASCs were isolated from 32 patients of different ages (range 26–62 years, average 43.2 ± 9.7). Live ASCs isolated ranged from 0 to 95.5 × 104 cells/g adipose, average 63.8 ± 30.6, with no clear correlation between ASC isolation and patient age. (b) Live cell count was compared relative to patient age across 4 cohort groups: <40 years (N = 12) versus ≥40 years (N = 20); <50 years (N = 23) versus ≥50 years (N = 9); and <40 years (N = 12) versus ≥50 years (N = 9). No significant differences in live ASC isolation were observed between any of the age cohorts; P > 0.05.
Mentions: Live ASCs isolated ranged from 0 to 5.9 × 104 cells/g tissue, averaging 2.95 × 104 ± 2.5 × 104 cells/g tissue with no clear correlation between ASC isolation and patient age (Figure 4). The initial live cells were counted for each frozen tissue sample and compared between the following age cohort pairs: <40 years versus ≥40 years (2.65 × 104 ± 1.8 × 104 cells/g tissue and 2.6 × 104 ± 1.65 × 104 cells/g tissue, resp.), <50 years versus ≥50 years (2.44 × 104 ± 1.48 × 104 cell/g tissue and 3.07 × 104 ± 2.14 × 104 cells/g tissue, resp.), and <40 years versus ≥50 years (2.65 × 104 ± 1.8 × 104 cells/g tissue and 3.07 × 104 ± 2.14 × 104 cells/g tissue, resp.). No significant differences in live ASC isolation were observed between any of the age cohorts, P > 0.05 (Figure 4).

Bottom Line: Significantly more viable cells were initially isolated from tissue cryopreserved <1 year than from tissue cryopreserved >2 years, irrespective of patient age.Mesenchymal stem cell markers were maintained in all cohorts tested throughout the duration of the study.Patient age does not significantly impact stem cell isolation, viability, or growth.

View Article: PubMed Central - PubMed

Affiliation: Thomas Jefferson University Hospital, 132 S 10th Street No. 763J, Philadelphia, PA 19107, USA.

ABSTRACT
We examined cell isolation, viability, and growth in adipose-derived stem cells harvested from whole adipose tissue subject to different cryopreservation lengths (2-1159 days) from patients of varying ages (26-62 years). Subcutaneous abdominal adipose tissue was excised during abdominoplasties and was cryopreserved. The viability and number of adipose-derived stem cells isolated were measured after initial isolation and after 9, 18, and 28 days of growth. Data were analyzed with respect to cryopreservation duration and patient age. Significantly more viable cells were initially isolated from tissue cryopreserved <1 year than from tissue cryopreserved >2 years, irrespective of patient age. However, this difference did not persist with continued growth and there were no significant differences in cell viability or growth at subsequent time points with respect to cryopreservation duration or patient age. Mesenchymal stem cell markers were maintained in all cohorts tested throughout the duration of the study. Consequently, longer cryopreservation negatively impacts initial live adipose-derived stem cell isolation; however, this effect is neutralized with continued cell growth. Patient age does not significantly impact stem cell isolation, viability, or growth. Cryopreservation of adipose tissue is an effective long-term banking method for isolation of adipose-derived stem cells in patients of varying ages.

No MeSH data available.