Limits...
Proposal of a Screening MIRU-VNTR Panel for the Preliminary Genotyping of Mycobacterium bovis in Mexico.

Bolado-Martínez E, Benavides-Dávila I, Candia-Plata Mdel C, Navarro-Navarro M, Avilés-Acosta M, Álvarez-Hernández G - Biomed Res Int (2015)

Bottom Line: In this study, 65 isolates of M. bovis obtained from clinical bovine samples proceeding from different geographic regions of Mexico were identified by phenotypic and genotypic tests and subsequently genotyped by a 24-locus MIRU-VNTR panel.The most polymorphic loci were selected to build a panel with a high discriminatory power similar to the 24-locus panel results.A panel of seven elements (QUB 11a, MIRU 26, ETR-A, QUB 26, MIRU 16, MIRU 27, and MIRU 39) with the highest allelic diversity showed an appropriate differentiation.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Ciencias Químico Biológicas, Universidad de Sonora, Boulevard Luis Encinas y Rosales, 83000 Hermosillo, SON, Mexico.

ABSTRACT
Mycobacterium bovis is the major causative agent of bovine tuberculosis, one of the most relevant zoonoses in the world, and affects a wide range of wild and domesticated animals. Development of screening panels in mycobacterial genotyping, according to specific geographical regions, is strongly needed. The aim of this study is to select a panel, constituted by highly polymorphic MIRU-VNTR loci, to discriminate clinical isolates of M. bovis in Mexico. In this study, 65 isolates of M. bovis obtained from clinical bovine samples proceeding from different geographic regions of Mexico were identified by phenotypic and genotypic tests and subsequently genotyped by a 24-locus MIRU-VNTR panel. The most polymorphic loci were selected to build a panel with a high discriminatory power similar to the 24-locus panel results. A panel of seven elements (QUB 11a, MIRU 26, ETR-A, QUB 26, MIRU 16, MIRU 27, and MIRU 39) with the highest allelic diversity showed an appropriate differentiation. The selected MIRU-VNTR elements, according to the regional allelic variability, may be used in the preliminary genotyping of Mycobacterium bovis isolates in Mexico.

No MeSH data available.


Related in: MedlinePlus

Map of Mexico and geographical origin of the 65 Mycobacterium bovis isolates included in this study.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4402168&req=5

fig1: Map of Mexico and geographical origin of the 65 Mycobacterium bovis isolates included in this study.

Mentions: Sixty-five M. bovis isolates, originally cultured from bovine tuberculosis lesions, were included in this study. The isolates were obtained from five states of Mexico (Figure 1), Baja California (11 isolates), Baja California Sur (5), Colima (12), Nayarit (12), Sinaloa (12), and Sonora (13), from 2010 to 2013 and sent to a reference laboratory (Laboratorio Estatal de Salud Pública) in Sonora, México. All isolates were identified as M. bovis by phenotypic (standard biochemical assays) [15] and genotypic (gyrB-RFLP and RD1) tests [16, 17]. All isolates were frozen in skim milk and kept at −20°C until use. Mycobacterium tuberculosis H37Rv strain was used as control.


Proposal of a Screening MIRU-VNTR Panel for the Preliminary Genotyping of Mycobacterium bovis in Mexico.

Bolado-Martínez E, Benavides-Dávila I, Candia-Plata Mdel C, Navarro-Navarro M, Avilés-Acosta M, Álvarez-Hernández G - Biomed Res Int (2015)

Map of Mexico and geographical origin of the 65 Mycobacterium bovis isolates included in this study.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4402168&req=5

fig1: Map of Mexico and geographical origin of the 65 Mycobacterium bovis isolates included in this study.
Mentions: Sixty-five M. bovis isolates, originally cultured from bovine tuberculosis lesions, were included in this study. The isolates were obtained from five states of Mexico (Figure 1), Baja California (11 isolates), Baja California Sur (5), Colima (12), Nayarit (12), Sinaloa (12), and Sonora (13), from 2010 to 2013 and sent to a reference laboratory (Laboratorio Estatal de Salud Pública) in Sonora, México. All isolates were identified as M. bovis by phenotypic (standard biochemical assays) [15] and genotypic (gyrB-RFLP and RD1) tests [16, 17]. All isolates were frozen in skim milk and kept at −20°C until use. Mycobacterium tuberculosis H37Rv strain was used as control.

Bottom Line: In this study, 65 isolates of M. bovis obtained from clinical bovine samples proceeding from different geographic regions of Mexico were identified by phenotypic and genotypic tests and subsequently genotyped by a 24-locus MIRU-VNTR panel.The most polymorphic loci were selected to build a panel with a high discriminatory power similar to the 24-locus panel results.A panel of seven elements (QUB 11a, MIRU 26, ETR-A, QUB 26, MIRU 16, MIRU 27, and MIRU 39) with the highest allelic diversity showed an appropriate differentiation.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Ciencias Químico Biológicas, Universidad de Sonora, Boulevard Luis Encinas y Rosales, 83000 Hermosillo, SON, Mexico.

ABSTRACT
Mycobacterium bovis is the major causative agent of bovine tuberculosis, one of the most relevant zoonoses in the world, and affects a wide range of wild and domesticated animals. Development of screening panels in mycobacterial genotyping, according to specific geographical regions, is strongly needed. The aim of this study is to select a panel, constituted by highly polymorphic MIRU-VNTR loci, to discriminate clinical isolates of M. bovis in Mexico. In this study, 65 isolates of M. bovis obtained from clinical bovine samples proceeding from different geographic regions of Mexico were identified by phenotypic and genotypic tests and subsequently genotyped by a 24-locus MIRU-VNTR panel. The most polymorphic loci were selected to build a panel with a high discriminatory power similar to the 24-locus panel results. A panel of seven elements (QUB 11a, MIRU 26, ETR-A, QUB 26, MIRU 16, MIRU 27, and MIRU 39) with the highest allelic diversity showed an appropriate differentiation. The selected MIRU-VNTR elements, according to the regional allelic variability, may be used in the preliminary genotyping of Mycobacterium bovis isolates in Mexico.

No MeSH data available.


Related in: MedlinePlus