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Effects of human arylamine N-acetyltransferase I knockdown in triple-negative breast cancer cell lines.

Tiang JM, Butcher NJ, Minchin RF - Cancer Med (2015)

Bottom Line: The expression of Snail increased when NAT1 was knocked down, while other genes associated with mesenchymal to epithelial transition (vimentin, cytokeratin-18, and Twist) did not show any changes.By contrast, both N-cadherin and β-catenin were significantly reduced.When MDA-MB-231 cells expressing shRNA were injected in vivo into BALB/c nu/nu nude mice, a significant reduction in the number of colonies that formed in the lungs was observed.

View Article: PubMed Central - PubMed

Affiliation: School of Biomedical Sciences, University of Queensland, St Lucia, Queensland, 4072, Australia.

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Related in: MedlinePlus

Loss of arylamine N-acetyltransferase I (NAT1) reduces the number of filopodia. (A) MDA-MB-231 cells stained with phalloidin show a decrease in filopodia protrusions following NAT1 knockdown (red = phalloidin, green = EGFP). (B) The number of filopodia was significantly reduced in the NAT1 knockdown cells compared to the control cells. Results are mean ± SEM, n = 9. Asterisk indicates P < 0.05. (C) Reintroduction of NAT1 into the shNAT1 cells rescued the effect of NAT1 knockdown on filopodia and cell morphology (red = phalloidin, green = FLAG-NAT1).
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fig04: Loss of arylamine N-acetyltransferase I (NAT1) reduces the number of filopodia. (A) MDA-MB-231 cells stained with phalloidin show a decrease in filopodia protrusions following NAT1 knockdown (red = phalloidin, green = EGFP). (B) The number of filopodia was significantly reduced in the NAT1 knockdown cells compared to the control cells. Results are mean ± SEM, n = 9. Asterisk indicates P < 0.05. (C) Reintroduction of NAT1 into the shNAT1 cells rescued the effect of NAT1 knockdown on filopodia and cell morphology (red = phalloidin, green = FLAG-NAT1).

Mentions: Since a decrease in NAT1 activity induced a more rounded cell morphology and inhibited in vitro cell invasion, we investigated the cytoskeleton structure of the cells. NAT1 knockdown cells displayed significantly less filopodia compared to the control cells (Fig.4A). Quantification of filopodia showed that loss of NAT1 reduced the number of filopodia on each cell to less than 35% of controls (Fig.4B).


Effects of human arylamine N-acetyltransferase I knockdown in triple-negative breast cancer cell lines.

Tiang JM, Butcher NJ, Minchin RF - Cancer Med (2015)

Loss of arylamine N-acetyltransferase I (NAT1) reduces the number of filopodia. (A) MDA-MB-231 cells stained with phalloidin show a decrease in filopodia protrusions following NAT1 knockdown (red = phalloidin, green = EGFP). (B) The number of filopodia was significantly reduced in the NAT1 knockdown cells compared to the control cells. Results are mean ± SEM, n = 9. Asterisk indicates P < 0.05. (C) Reintroduction of NAT1 into the shNAT1 cells rescued the effect of NAT1 knockdown on filopodia and cell morphology (red = phalloidin, green = FLAG-NAT1).
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4402071&req=5

fig04: Loss of arylamine N-acetyltransferase I (NAT1) reduces the number of filopodia. (A) MDA-MB-231 cells stained with phalloidin show a decrease in filopodia protrusions following NAT1 knockdown (red = phalloidin, green = EGFP). (B) The number of filopodia was significantly reduced in the NAT1 knockdown cells compared to the control cells. Results are mean ± SEM, n = 9. Asterisk indicates P < 0.05. (C) Reintroduction of NAT1 into the shNAT1 cells rescued the effect of NAT1 knockdown on filopodia and cell morphology (red = phalloidin, green = FLAG-NAT1).
Mentions: Since a decrease in NAT1 activity induced a more rounded cell morphology and inhibited in vitro cell invasion, we investigated the cytoskeleton structure of the cells. NAT1 knockdown cells displayed significantly less filopodia compared to the control cells (Fig.4A). Quantification of filopodia showed that loss of NAT1 reduced the number of filopodia on each cell to less than 35% of controls (Fig.4B).

Bottom Line: The expression of Snail increased when NAT1 was knocked down, while other genes associated with mesenchymal to epithelial transition (vimentin, cytokeratin-18, and Twist) did not show any changes.By contrast, both N-cadherin and β-catenin were significantly reduced.When MDA-MB-231 cells expressing shRNA were injected in vivo into BALB/c nu/nu nude mice, a significant reduction in the number of colonies that formed in the lungs was observed.

View Article: PubMed Central - PubMed

Affiliation: School of Biomedical Sciences, University of Queensland, St Lucia, Queensland, 4072, Australia.

Show MeSH
Related in: MedlinePlus