Effects of human arylamine N-acetyltransferase I knockdown in triple-negative breast cancer cell lines.
Bottom Line: The expression of Snail increased when NAT1 was knocked down, while other genes associated with mesenchymal to epithelial transition (vimentin, cytokeratin-18, and Twist) did not show any changes.By contrast, both N-cadherin and β-catenin were significantly reduced.When MDA-MB-231 cells expressing shRNA were injected in vivo into BALB/c nu/nu nude mice, a significant reduction in the number of colonies that formed in the lungs was observed.
Affiliation: School of Biomedical Sciences, University of Queensland, St Lucia, Queensland, 4072, Australia.Show MeSH
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Mentions: Since a decrease in NAT1 activity induced a more rounded cell morphology and inhibited in vitro cell invasion, we investigated the cytoskeleton structure of the cells. NAT1 knockdown cells displayed significantly less filopodia compared to the control cells (Fig.4A). Quantification of filopodia showed that loss of NAT1 reduced the number of filopodia on each cell to less than 35% of controls (Fig.4B).
Affiliation: School of Biomedical Sciences, University of Queensland, St Lucia, Queensland, 4072, Australia.