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Protein enrichment of an Opuntia ficus-indica cladode hydrolysate by cultivation of Candida utilis and Kluyveromyces marxianus.

Akanni GB, du Preez JC, Steyn L, Kilian SG - J. Sci. Food Agric. (2014)

Bottom Line: Yeast cultivation more than doubled the protein content of the hydrolysate, with an essential amino acid profile superior to sorghum and millet grains.Despite its low biomass yield, its performance compared well with C. utilis.This is the first report showing that the protein content and quality of O. ficus-indica cladode biomass could substantially be improved by yeast cultivation, including a comparative evaluation of C. utilis and K. marxianus.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, Bloemfontein 9300, South Africa.

No MeSH data available.


Related in: MedlinePlus

Cultivation profiles of C. utilis (A) and K. marxianus (B) with the DOT controlled above 30% of saturation in an enzymatic hydrolysate of O. ficus-indica cladode biomass supplemented with mineral salts.
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fig01: Cultivation profiles of C. utilis (A) and K. marxianus (B) with the DOT controlled above 30% of saturation in an enzymatic hydrolysate of O. ficus-indica cladode biomass supplemented with mineral salts.

Mentions: In the hydrolysate medium K. marxianus and C. utilis preferentially utilized glucose followed by fructose uptake (Fig. 1). After 50 h of cultivation K. marxianus had consumed 97% and C. utilis only 74% of the total sugars. K. marxianus utilized galactose, arabinose and xylose mainly after exhaustion of the glucose and, with the exception of arabinose, the sugars were almost completely consumed within 30 h. Candida utilis consumed glucose, fructose and xylose within 35 h, but at the end of the cultivation only 32% of the galactose and 11% of the arabinose had been consumed (Fig. 1). The volumetric rate of total sugar uptake by K. marxianus was 2.63 g L−1 h−1, which was substantially faster than C. utilis (Table 2). Candida utilis produced small amounts of glycerol and acetic acid, whereas K. marxianus produced up to 2.9 g ethanol L−1 as well as low concentrations of acetic acid and ethyl acetate, despite the aerobic culture conditions (Table 2).


Protein enrichment of an Opuntia ficus-indica cladode hydrolysate by cultivation of Candida utilis and Kluyveromyces marxianus.

Akanni GB, du Preez JC, Steyn L, Kilian SG - J. Sci. Food Agric. (2014)

Cultivation profiles of C. utilis (A) and K. marxianus (B) with the DOT controlled above 30% of saturation in an enzymatic hydrolysate of O. ficus-indica cladode biomass supplemented with mineral salts.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4402007&req=5

fig01: Cultivation profiles of C. utilis (A) and K. marxianus (B) with the DOT controlled above 30% of saturation in an enzymatic hydrolysate of O. ficus-indica cladode biomass supplemented with mineral salts.
Mentions: In the hydrolysate medium K. marxianus and C. utilis preferentially utilized glucose followed by fructose uptake (Fig. 1). After 50 h of cultivation K. marxianus had consumed 97% and C. utilis only 74% of the total sugars. K. marxianus utilized galactose, arabinose and xylose mainly after exhaustion of the glucose and, with the exception of arabinose, the sugars were almost completely consumed within 30 h. Candida utilis consumed glucose, fructose and xylose within 35 h, but at the end of the cultivation only 32% of the galactose and 11% of the arabinose had been consumed (Fig. 1). The volumetric rate of total sugar uptake by K. marxianus was 2.63 g L−1 h−1, which was substantially faster than C. utilis (Table 2). Candida utilis produced small amounts of glycerol and acetic acid, whereas K. marxianus produced up to 2.9 g ethanol L−1 as well as low concentrations of acetic acid and ethyl acetate, despite the aerobic culture conditions (Table 2).

Bottom Line: Yeast cultivation more than doubled the protein content of the hydrolysate, with an essential amino acid profile superior to sorghum and millet grains.Despite its low biomass yield, its performance compared well with C. utilis.This is the first report showing that the protein content and quality of O. ficus-indica cladode biomass could substantially be improved by yeast cultivation, including a comparative evaluation of C. utilis and K. marxianus.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, Bloemfontein 9300, South Africa.

No MeSH data available.


Related in: MedlinePlus