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Identification of Entamoeba histolytica by Molecular Method in Surface Water of Rasht City, Iran.

Hemmati A, Hooshmand E, Hosseini MJ - Iran. J. Public Health (2015)

Bottom Line: In this cross-sectional study, 49 water samples including 18 rivers and 6 wetlands were collected from different regions near the city of Rasht in autumn of 2012.In the molecular analysis, contamination by E. histolytica was proved in the waters of Rasht City.Further investigations including more samples and necessary preparations must be applied to prevent contamination.

View Article: PubMed Central - PubMed

Affiliation: Dep. of Microbiology, Rasht Branch, Islamic Azad University, Rasht, Iran.

ABSTRACT

Background: This study was conducted with the aim of determining surface water contamination with cysts of Entamoeba histolytica using PCR in Rasht City, Northern Iran.

Methods: In this cross-sectional study, 49 water samples including 18 rivers and 6 wetlands were collected from different regions near the city of Rasht in autumn of 2012. After filtration using 0.22 μm nitrate cellulose membrane filters, the samples were examined using microscope and PCR method.

Results: In microscopic examination, four samples of the 49 samples were positive for cysts of E. (histolytica / dispar / muschkovskii). By using PCR method and molecular analysis, one sample was positive for E. histolytica.

Conclusion: In the molecular analysis, contamination by E. histolytica was proved in the waters of Rasht City. Further investigations including more samples and necessary preparations must be applied to prevent contamination.

No MeSH data available.


Related in: MedlinePlus

PCR amplification of samples DNA with the Entamoeba histolytica specific primersLane M: molecular marker (100 bp) ladders, C+: positive control (E. histolytica DNA), C−: negative control (H2O), Lane 1: amplified product (220 bp) indicating positive sample, Lanes 2–4: Positive Samples in microscopic examination that were not amplified by PCR.
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Figure 1: PCR amplification of samples DNA with the Entamoeba histolytica specific primersLane M: molecular marker (100 bp) ladders, C+: positive control (E. histolytica DNA), C−: negative control (H2O), Lane 1: amplified product (220 bp) indicating positive sample, Lanes 2–4: Positive Samples in microscopic examination that were not amplified by PCR.

Mentions: In microscopic examination, four samples of the 49 samples were positive for cysts of Entamoeba (histolytica / dispar / muschkovskii). These three species cannot be differentiated by microscopy and can only be differentiated by the use of molecular methods. By using PCR method, one sample was positive for E. histolytica. Just as we expected, in one sample in addition to positive control that was Genomic DNA of E. histolytica (HM-1: IMSS), had a band with 220 bp weight (Fig. 1).


Identification of Entamoeba histolytica by Molecular Method in Surface Water of Rasht City, Iran.

Hemmati A, Hooshmand E, Hosseini MJ - Iran. J. Public Health (2015)

PCR amplification of samples DNA with the Entamoeba histolytica specific primersLane M: molecular marker (100 bp) ladders, C+: positive control (E. histolytica DNA), C−: negative control (H2O), Lane 1: amplified product (220 bp) indicating positive sample, Lanes 2–4: Positive Samples in microscopic examination that were not amplified by PCR.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4401882&req=5

Figure 1: PCR amplification of samples DNA with the Entamoeba histolytica specific primersLane M: molecular marker (100 bp) ladders, C+: positive control (E. histolytica DNA), C−: negative control (H2O), Lane 1: amplified product (220 bp) indicating positive sample, Lanes 2–4: Positive Samples in microscopic examination that were not amplified by PCR.
Mentions: In microscopic examination, four samples of the 49 samples were positive for cysts of Entamoeba (histolytica / dispar / muschkovskii). These three species cannot be differentiated by microscopy and can only be differentiated by the use of molecular methods. By using PCR method, one sample was positive for E. histolytica. Just as we expected, in one sample in addition to positive control that was Genomic DNA of E. histolytica (HM-1: IMSS), had a band with 220 bp weight (Fig. 1).

Bottom Line: In this cross-sectional study, 49 water samples including 18 rivers and 6 wetlands were collected from different regions near the city of Rasht in autumn of 2012.In the molecular analysis, contamination by E. histolytica was proved in the waters of Rasht City.Further investigations including more samples and necessary preparations must be applied to prevent contamination.

View Article: PubMed Central - PubMed

Affiliation: Dep. of Microbiology, Rasht Branch, Islamic Azad University, Rasht, Iran.

ABSTRACT

Background: This study was conducted with the aim of determining surface water contamination with cysts of Entamoeba histolytica using PCR in Rasht City, Northern Iran.

Methods: In this cross-sectional study, 49 water samples including 18 rivers and 6 wetlands were collected from different regions near the city of Rasht in autumn of 2012. After filtration using 0.22 μm nitrate cellulose membrane filters, the samples were examined using microscope and PCR method.

Results: In microscopic examination, four samples of the 49 samples were positive for cysts of E. (histolytica / dispar / muschkovskii). By using PCR method and molecular analysis, one sample was positive for E. histolytica.

Conclusion: In the molecular analysis, contamination by E. histolytica was proved in the waters of Rasht City. Further investigations including more samples and necessary preparations must be applied to prevent contamination.

No MeSH data available.


Related in: MedlinePlus