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Identification and profiling of microRNAs in goat endometrium during embryo implantation.

Song Y, An X, Zhang L, Fu M, Peng J, Han P, Hou J, Zhou Z, Cao B - PLoS ONE (2015)

Bottom Line: There were 110 up-expressed miRNAs and 33 down-expressed miRNAs in receptive endometrium compared with the pre-receptive endometrium meeting the criteria of P-values< 0.05.Moreover, GO and KEGG analysis of the target genes of the differentially expressed miRNAs revealed some candidate miRNAs, genes and pathways that may involve in the formation of the receptive endometrium.Based on stem-loop RT-qPCR, 15 miRNAs were detected and the results suggested that the majority of the miRNA expression data measured by Solexa deep sequencing could represent actual miRNA expression levels.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, P.R. China.

ABSTRACT

Background: MicroRNAs (miRNAs) are short, highly conserved small noncoding RNAs that had fundamental roles in post-transcriptional gene expression, and they are crucial for proper control of biological processes and known to participate in embryo implantation. However, miRNA expression profiles in the pre-receptive and receptive phases of the goat endometrium during embryo implantation are unknown.

Results: A total of 1,069 and 847 miRNAs were expressed in receptive (R) and pre-receptive (P) goat endometrium, and 632 miRNAs were co-expressed in both phases. We identified 545 (50.98%) known miRNAs in the R library and 522 (61.63%) in the P library. There were 110 up-expressed miRNAs and 33 down-expressed miRNAs in receptive endometrium compared with the pre-receptive endometrium meeting the criteria of P-values< 0.05. Moreover, GO and KEGG analysis of the target genes of the differentially expressed miRNAs revealed some candidate miRNAs, genes and pathways that may involve in the formation of the receptive endometrium. Based on stem-loop RT-qPCR, 15 miRNAs were detected and the results suggested that the majority of the miRNA expression data measured by Solexa deep sequencing could represent actual miRNA expression levels.

Conclusions: Our data revealed the first miRNA profile related to the biology of the goat receptive endometrium during embryo implantation, and the results suggested that a subset of miRNAs might play important roles in the formation of endometrial receptivity. Thus, elucidating the physiological roles of endometrial miRNAs will help us better understand the genetic control of embryo implantation in goats.

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The differential expression levels of miRNAs between receptive and pre-receptive endometrium in goat.Note: Expression level (1): Expression level of pre-receptive endometrium; Expression level (2): Expression level of receptive endometrium. Each point in the Fig. represents a miRNA. Red points represent miRNAs with a fold change > 2, blue points represent miRNAs with 1/2 < fold change ≤ 2, green points represent miRNAs with fold change ≤ 1/2.
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pone.0122202.g004: The differential expression levels of miRNAs between receptive and pre-receptive endometrium in goat.Note: Expression level (1): Expression level of pre-receptive endometrium; Expression level (2): Expression level of receptive endometrium. Each point in the Fig. represents a miRNA. Red points represent miRNAs with a fold change > 2, blue points represent miRNAs with 1/2 < fold change ≤ 2, green points represent miRNAs with fold change ≤ 1/2.

Mentions: Pair-wise comparisons revealed important differentially expressed miRNAs between each developmental phase. Although some of their expression quantities were equivalent, there were some miRNAs expressed differently between the pre-receptive and receptive phases. We focused our attention on miRNAs meeting our designated criteria of P-values< 0.05 and /log2 (fold change)/> 1, and a total of 143 differentially expressed miRNAs were selected from the P and R libraries (Table 6). There were 33 differentially expressed miRNAs that were down-regulated in the receptive endometrium compared with pre-receptive endometrium in goats (fold change≤ 0.5), 231 miRNAs with fold changes were greater than 0.5 and less than 2 (0.5 <fold change≤ 2), and 110 miRNAs with fold changes greater than 2 (fold change> 2) (Fig 4). It deserved to note that there were some miRNAs expressed specifically, such as bta-miR-431_R-3 was detected only in the receptive endometrium and bta-miR-216a_R-2 in the pre-receptive endometrium (Table 6).


Identification and profiling of microRNAs in goat endometrium during embryo implantation.

Song Y, An X, Zhang L, Fu M, Peng J, Han P, Hou J, Zhou Z, Cao B - PLoS ONE (2015)

The differential expression levels of miRNAs between receptive and pre-receptive endometrium in goat.Note: Expression level (1): Expression level of pre-receptive endometrium; Expression level (2): Expression level of receptive endometrium. Each point in the Fig. represents a miRNA. Red points represent miRNAs with a fold change > 2, blue points represent miRNAs with 1/2 < fold change ≤ 2, green points represent miRNAs with fold change ≤ 1/2.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4401794&req=5

pone.0122202.g004: The differential expression levels of miRNAs between receptive and pre-receptive endometrium in goat.Note: Expression level (1): Expression level of pre-receptive endometrium; Expression level (2): Expression level of receptive endometrium. Each point in the Fig. represents a miRNA. Red points represent miRNAs with a fold change > 2, blue points represent miRNAs with 1/2 < fold change ≤ 2, green points represent miRNAs with fold change ≤ 1/2.
Mentions: Pair-wise comparisons revealed important differentially expressed miRNAs between each developmental phase. Although some of their expression quantities were equivalent, there were some miRNAs expressed differently between the pre-receptive and receptive phases. We focused our attention on miRNAs meeting our designated criteria of P-values< 0.05 and /log2 (fold change)/> 1, and a total of 143 differentially expressed miRNAs were selected from the P and R libraries (Table 6). There were 33 differentially expressed miRNAs that were down-regulated in the receptive endometrium compared with pre-receptive endometrium in goats (fold change≤ 0.5), 231 miRNAs with fold changes were greater than 0.5 and less than 2 (0.5 <fold change≤ 2), and 110 miRNAs with fold changes greater than 2 (fold change> 2) (Fig 4). It deserved to note that there were some miRNAs expressed specifically, such as bta-miR-431_R-3 was detected only in the receptive endometrium and bta-miR-216a_R-2 in the pre-receptive endometrium (Table 6).

Bottom Line: There were 110 up-expressed miRNAs and 33 down-expressed miRNAs in receptive endometrium compared with the pre-receptive endometrium meeting the criteria of P-values< 0.05.Moreover, GO and KEGG analysis of the target genes of the differentially expressed miRNAs revealed some candidate miRNAs, genes and pathways that may involve in the formation of the receptive endometrium.Based on stem-loop RT-qPCR, 15 miRNAs were detected and the results suggested that the majority of the miRNA expression data measured by Solexa deep sequencing could represent actual miRNA expression levels.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, P.R. China.

ABSTRACT

Background: MicroRNAs (miRNAs) are short, highly conserved small noncoding RNAs that had fundamental roles in post-transcriptional gene expression, and they are crucial for proper control of biological processes and known to participate in embryo implantation. However, miRNA expression profiles in the pre-receptive and receptive phases of the goat endometrium during embryo implantation are unknown.

Results: A total of 1,069 and 847 miRNAs were expressed in receptive (R) and pre-receptive (P) goat endometrium, and 632 miRNAs were co-expressed in both phases. We identified 545 (50.98%) known miRNAs in the R library and 522 (61.63%) in the P library. There were 110 up-expressed miRNAs and 33 down-expressed miRNAs in receptive endometrium compared with the pre-receptive endometrium meeting the criteria of P-values< 0.05. Moreover, GO and KEGG analysis of the target genes of the differentially expressed miRNAs revealed some candidate miRNAs, genes and pathways that may involve in the formation of the receptive endometrium. Based on stem-loop RT-qPCR, 15 miRNAs were detected and the results suggested that the majority of the miRNA expression data measured by Solexa deep sequencing could represent actual miRNA expression levels.

Conclusions: Our data revealed the first miRNA profile related to the biology of the goat receptive endometrium during embryo implantation, and the results suggested that a subset of miRNAs might play important roles in the formation of endometrial receptivity. Thus, elucidating the physiological roles of endometrial miRNAs will help us better understand the genetic control of embryo implantation in goats.

Show MeSH