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ABA inducible rice protein phosphatase 2C confers ABA insensitivity and abiotic stress tolerance in Arabidopsis.

Singh A, Jha SK, Bagri J, Pandey GK - PLoS ONE (2015)

Bottom Line: At adult stage, OsPP108 overexpression leads to high tolerance to salt, mannitol and drought stresses with far better physiological parameters such as water loss, fresh weight, chlorophyll content and photosynthetic potential (Fv/Fm) in transgenic Arabidopsis plants.Expression profile of various stress marker genes in OsPP108 overexpressing plants revealed interplay of ABA dependent and independent pathway for abiotic stress tolerance.Overall, this study has identified a potential rice group A PP2C, which regulates ABA signaling negatively and abiotic stress signaling positively.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, Dhaula Kuan, New Delhi, India.

ABSTRACT
Arabidopsis PP2C belonging to group A have been extensively worked out and known to negatively regulate ABA signaling. However, rice (Oryza sativa) orthologs of Arabidopsis group A PP2C are scarcely characterized functionally. We have identified a group A PP2C from rice (OsPP108), which is highly inducible under ABA, salt and drought stresses and localized predominantly in the nucleus. Genetic analysis revealed that Arabidopsis plants overexpressing OsPP108 are highly insensitive to ABA and tolerant to high salt and mannitol stresses during seed germination, root growth and overall seedling growth. At adult stage, OsPP108 overexpression leads to high tolerance to salt, mannitol and drought stresses with far better physiological parameters such as water loss, fresh weight, chlorophyll content and photosynthetic potential (Fv/Fm) in transgenic Arabidopsis plants. Expression profile of various stress marker genes in OsPP108 overexpressing plants revealed interplay of ABA dependent and independent pathway for abiotic stress tolerance. Overall, this study has identified a potential rice group A PP2C, which regulates ABA signaling negatively and abiotic stress signaling positively. Transgenic rice plants overexpressing this gene might provide an answer to the problem of low crop yield and productivity during adverse environmental conditions.

No MeSH data available.


Related in: MedlinePlus

Expression profile of OsPP108 under ABA and abiotic stresses.qPCR analysis was performed to generate the expression profile of OsPP108 under ABA, salt, drought and cold treatment in rice seedlings. Different time period of treatment in hours is indicated on X-axis and fold change in expression level w.r.t. untreated control sample is indicated on Y-axis. Each bar represents mean value of two replicates. Standard error among the samples is indicated by error bars. * represents p-value < 0.05 and ** p-value < 0.01 for treated samples w.r.t. untreated control (0h).
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pone.0125168.g002: Expression profile of OsPP108 under ABA and abiotic stresses.qPCR analysis was performed to generate the expression profile of OsPP108 under ABA, salt, drought and cold treatment in rice seedlings. Different time period of treatment in hours is indicated on X-axis and fold change in expression level w.r.t. untreated control sample is indicated on Y-axis. Each bar represents mean value of two replicates. Standard error among the samples is indicated by error bars. * represents p-value < 0.05 and ** p-value < 0.01 for treated samples w.r.t. untreated control (0h).

Mentions: In our previous study, OsPP108 was found to be up-regulated under abiotic stresses such as salt and drought after 3 hrs of stress treatment [6]. Since OsPP108 belongs to group A of PP2Cs, it was of interest to investigate its expression pattern under ABA and abiotic stress treatments. Therefore, we performed detail expression kinetics in rice seedlings by qPCR. Expression profile revealed that OsPP108 was highly induced by ABA after 3 hrs of treatment and induction was about 30 fold higher in comparison to the untreated seedlings, and it subside to 12 fold and 7 folds in 6 hrs and 12 hrs, respectively (Fig 2). Similar expression pattern was suggested for OsPP108 under ABA treatment by the microarray expression profile obtain from RiceXPro database (S1 Fig). Long term drought stress for 6 hrs leads to up-regulation of the transcript level to 60 folds with a significant up-regulation of 11 fold and 18 fold during 1 hrs and 3hrs of drought stress, respectively. OsPP108 was also induced by salt stress treatment with approx. 6 fold up-regulation after 3 hrs, about 15 fold up-regulation after 6 hrs, and 3 fold up-regulation after 12 hours of treatment. Slight down-regulation was observed for OsPP108 after 3 hrs cold treatment, but no significant transcript fluctuations observed after 6 and 12 hrs.


ABA inducible rice protein phosphatase 2C confers ABA insensitivity and abiotic stress tolerance in Arabidopsis.

Singh A, Jha SK, Bagri J, Pandey GK - PLoS ONE (2015)

Expression profile of OsPP108 under ABA and abiotic stresses.qPCR analysis was performed to generate the expression profile of OsPP108 under ABA, salt, drought and cold treatment in rice seedlings. Different time period of treatment in hours is indicated on X-axis and fold change in expression level w.r.t. untreated control sample is indicated on Y-axis. Each bar represents mean value of two replicates. Standard error among the samples is indicated by error bars. * represents p-value < 0.05 and ** p-value < 0.01 for treated samples w.r.t. untreated control (0h).
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4401787&req=5

pone.0125168.g002: Expression profile of OsPP108 under ABA and abiotic stresses.qPCR analysis was performed to generate the expression profile of OsPP108 under ABA, salt, drought and cold treatment in rice seedlings. Different time period of treatment in hours is indicated on X-axis and fold change in expression level w.r.t. untreated control sample is indicated on Y-axis. Each bar represents mean value of two replicates. Standard error among the samples is indicated by error bars. * represents p-value < 0.05 and ** p-value < 0.01 for treated samples w.r.t. untreated control (0h).
Mentions: In our previous study, OsPP108 was found to be up-regulated under abiotic stresses such as salt and drought after 3 hrs of stress treatment [6]. Since OsPP108 belongs to group A of PP2Cs, it was of interest to investigate its expression pattern under ABA and abiotic stress treatments. Therefore, we performed detail expression kinetics in rice seedlings by qPCR. Expression profile revealed that OsPP108 was highly induced by ABA after 3 hrs of treatment and induction was about 30 fold higher in comparison to the untreated seedlings, and it subside to 12 fold and 7 folds in 6 hrs and 12 hrs, respectively (Fig 2). Similar expression pattern was suggested for OsPP108 under ABA treatment by the microarray expression profile obtain from RiceXPro database (S1 Fig). Long term drought stress for 6 hrs leads to up-regulation of the transcript level to 60 folds with a significant up-regulation of 11 fold and 18 fold during 1 hrs and 3hrs of drought stress, respectively. OsPP108 was also induced by salt stress treatment with approx. 6 fold up-regulation after 3 hrs, about 15 fold up-regulation after 6 hrs, and 3 fold up-regulation after 12 hours of treatment. Slight down-regulation was observed for OsPP108 after 3 hrs cold treatment, but no significant transcript fluctuations observed after 6 and 12 hrs.

Bottom Line: At adult stage, OsPP108 overexpression leads to high tolerance to salt, mannitol and drought stresses with far better physiological parameters such as water loss, fresh weight, chlorophyll content and photosynthetic potential (Fv/Fm) in transgenic Arabidopsis plants.Expression profile of various stress marker genes in OsPP108 overexpressing plants revealed interplay of ABA dependent and independent pathway for abiotic stress tolerance.Overall, this study has identified a potential rice group A PP2C, which regulates ABA signaling negatively and abiotic stress signaling positively.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, Dhaula Kuan, New Delhi, India.

ABSTRACT
Arabidopsis PP2C belonging to group A have been extensively worked out and known to negatively regulate ABA signaling. However, rice (Oryza sativa) orthologs of Arabidopsis group A PP2C are scarcely characterized functionally. We have identified a group A PP2C from rice (OsPP108), which is highly inducible under ABA, salt and drought stresses and localized predominantly in the nucleus. Genetic analysis revealed that Arabidopsis plants overexpressing OsPP108 are highly insensitive to ABA and tolerant to high salt and mannitol stresses during seed germination, root growth and overall seedling growth. At adult stage, OsPP108 overexpression leads to high tolerance to salt, mannitol and drought stresses with far better physiological parameters such as water loss, fresh weight, chlorophyll content and photosynthetic potential (Fv/Fm) in transgenic Arabidopsis plants. Expression profile of various stress marker genes in OsPP108 overexpressing plants revealed interplay of ABA dependent and independent pathway for abiotic stress tolerance. Overall, this study has identified a potential rice group A PP2C, which regulates ABA signaling negatively and abiotic stress signaling positively. Transgenic rice plants overexpressing this gene might provide an answer to the problem of low crop yield and productivity during adverse environmental conditions.

No MeSH data available.


Related in: MedlinePlus