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ABA inducible rice protein phosphatase 2C confers ABA insensitivity and abiotic stress tolerance in Arabidopsis.

Singh A, Jha SK, Bagri J, Pandey GK - PLoS ONE (2015)

Bottom Line: At adult stage, OsPP108 overexpression leads to high tolerance to salt, mannitol and drought stresses with far better physiological parameters such as water loss, fresh weight, chlorophyll content and photosynthetic potential (Fv/Fm) in transgenic Arabidopsis plants.Expression profile of various stress marker genes in OsPP108 overexpressing plants revealed interplay of ABA dependent and independent pathway for abiotic stress tolerance.Overall, this study has identified a potential rice group A PP2C, which regulates ABA signaling negatively and abiotic stress signaling positively.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, Dhaula Kuan, New Delhi, India.

ABSTRACT
Arabidopsis PP2C belonging to group A have been extensively worked out and known to negatively regulate ABA signaling. However, rice (Oryza sativa) orthologs of Arabidopsis group A PP2C are scarcely characterized functionally. We have identified a group A PP2C from rice (OsPP108), which is highly inducible under ABA, salt and drought stresses and localized predominantly in the nucleus. Genetic analysis revealed that Arabidopsis plants overexpressing OsPP108 are highly insensitive to ABA and tolerant to high salt and mannitol stresses during seed germination, root growth and overall seedling growth. At adult stage, OsPP108 overexpression leads to high tolerance to salt, mannitol and drought stresses with far better physiological parameters such as water loss, fresh weight, chlorophyll content and photosynthetic potential (Fv/Fm) in transgenic Arabidopsis plants. Expression profile of various stress marker genes in OsPP108 overexpressing plants revealed interplay of ABA dependent and independent pathway for abiotic stress tolerance. Overall, this study has identified a potential rice group A PP2C, which regulates ABA signaling negatively and abiotic stress signaling positively. Transgenic rice plants overexpressing this gene might provide an answer to the problem of low crop yield and productivity during adverse environmental conditions.

No MeSH data available.


Related in: MedlinePlus

Gene, protein structure and sequence analysis of OsPP108.(A) Localization of OsPP108 gene on chromosome 9 of rice, gene structure showing that OsPP108 is intronless and has 1077 bp open reading frame. Protein structure showing that OsPP108 protein is comprised of 359 amino acid residues and PP2C catalytic domain starts at amino acid 73 and ends at 345. (B) Phylogenetic analysis of group A PP2Cs from rice and Arabidopsis. Group A PP2Cs are further divided into two sub-clades a and b and OsPP108 belongs to sub-clade b. Tree was constructed with MEGA5, scale bar represents amino acid substitution per site. (C) Multiple sequence alignment of OsPP108 with the entire group A PP2Cs from Arabidopsis. Amino acid position is indicated at the bottom of the alignment. Multiple alignments were done in clustalX2.0.8 and manually edited to show the conservation clearly.
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pone.0125168.g001: Gene, protein structure and sequence analysis of OsPP108.(A) Localization of OsPP108 gene on chromosome 9 of rice, gene structure showing that OsPP108 is intronless and has 1077 bp open reading frame. Protein structure showing that OsPP108 protein is comprised of 359 amino acid residues and PP2C catalytic domain starts at amino acid 73 and ends at 345. (B) Phylogenetic analysis of group A PP2Cs from rice and Arabidopsis. Group A PP2Cs are further divided into two sub-clades a and b and OsPP108 belongs to sub-clade b. Tree was constructed with MEGA5, scale bar represents amino acid substitution per site. (C) Multiple sequence alignment of OsPP108 with the entire group A PP2Cs from Arabidopsis. Amino acid position is indicated at the bottom of the alignment. Multiple alignments were done in clustalX2.0.8 and manually edited to show the conservation clearly.

Mentions: Gene structure analysis showed that OsPP108 (RGAP Locus ID; LOC_Os09g15670) is an intronless gene located on chromosome 9 encoding 359 aa (amino acids) protein (Fig 1A). Domain analysis of OsPP108 protein, predicted the presence of PP2C catalytic domain, which spans 73–345 aa and authenticated the integrity of the protein. Phylogenetic analysis using domain sequences placed this gene in the group A of rice PP2Cs [6]. We performed the phylogenetic analysis only with group A PP2Cs, both from rice and Arabidopsis to get a more closer view, and it was found that OsPP108 falls in subgroup b of group A rice PP2Cs (Fig 1B). Previously, group A PP2Cs have been implicated in ABA signaling and responses in plants [3,11,13–15]. Therefore, we performed multiple sequence alignment of OsPP108 and clade A PP2Cs from Arabidopsis and it was observed that OsPP108 has high sequence homology with all the ABA related group A PP2Cs (Fig 1C). A high degree of sequence conservation with all the members of this group from two different plant species suggested possible involvement of OsPP108 in similar ABA related function and prompted us to perform in-depth functional characterization, to comprehend the role of this PP2C gene in ABA and abiotic stress mediated signaling pathway.


ABA inducible rice protein phosphatase 2C confers ABA insensitivity and abiotic stress tolerance in Arabidopsis.

Singh A, Jha SK, Bagri J, Pandey GK - PLoS ONE (2015)

Gene, protein structure and sequence analysis of OsPP108.(A) Localization of OsPP108 gene on chromosome 9 of rice, gene structure showing that OsPP108 is intronless and has 1077 bp open reading frame. Protein structure showing that OsPP108 protein is comprised of 359 amino acid residues and PP2C catalytic domain starts at amino acid 73 and ends at 345. (B) Phylogenetic analysis of group A PP2Cs from rice and Arabidopsis. Group A PP2Cs are further divided into two sub-clades a and b and OsPP108 belongs to sub-clade b. Tree was constructed with MEGA5, scale bar represents amino acid substitution per site. (C) Multiple sequence alignment of OsPP108 with the entire group A PP2Cs from Arabidopsis. Amino acid position is indicated at the bottom of the alignment. Multiple alignments were done in clustalX2.0.8 and manually edited to show the conservation clearly.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4401787&req=5

pone.0125168.g001: Gene, protein structure and sequence analysis of OsPP108.(A) Localization of OsPP108 gene on chromosome 9 of rice, gene structure showing that OsPP108 is intronless and has 1077 bp open reading frame. Protein structure showing that OsPP108 protein is comprised of 359 amino acid residues and PP2C catalytic domain starts at amino acid 73 and ends at 345. (B) Phylogenetic analysis of group A PP2Cs from rice and Arabidopsis. Group A PP2Cs are further divided into two sub-clades a and b and OsPP108 belongs to sub-clade b. Tree was constructed with MEGA5, scale bar represents amino acid substitution per site. (C) Multiple sequence alignment of OsPP108 with the entire group A PP2Cs from Arabidopsis. Amino acid position is indicated at the bottom of the alignment. Multiple alignments were done in clustalX2.0.8 and manually edited to show the conservation clearly.
Mentions: Gene structure analysis showed that OsPP108 (RGAP Locus ID; LOC_Os09g15670) is an intronless gene located on chromosome 9 encoding 359 aa (amino acids) protein (Fig 1A). Domain analysis of OsPP108 protein, predicted the presence of PP2C catalytic domain, which spans 73–345 aa and authenticated the integrity of the protein. Phylogenetic analysis using domain sequences placed this gene in the group A of rice PP2Cs [6]. We performed the phylogenetic analysis only with group A PP2Cs, both from rice and Arabidopsis to get a more closer view, and it was found that OsPP108 falls in subgroup b of group A rice PP2Cs (Fig 1B). Previously, group A PP2Cs have been implicated in ABA signaling and responses in plants [3,11,13–15]. Therefore, we performed multiple sequence alignment of OsPP108 and clade A PP2Cs from Arabidopsis and it was observed that OsPP108 has high sequence homology with all the ABA related group A PP2Cs (Fig 1C). A high degree of sequence conservation with all the members of this group from two different plant species suggested possible involvement of OsPP108 in similar ABA related function and prompted us to perform in-depth functional characterization, to comprehend the role of this PP2C gene in ABA and abiotic stress mediated signaling pathway.

Bottom Line: At adult stage, OsPP108 overexpression leads to high tolerance to salt, mannitol and drought stresses with far better physiological parameters such as water loss, fresh weight, chlorophyll content and photosynthetic potential (Fv/Fm) in transgenic Arabidopsis plants.Expression profile of various stress marker genes in OsPP108 overexpressing plants revealed interplay of ABA dependent and independent pathway for abiotic stress tolerance.Overall, this study has identified a potential rice group A PP2C, which regulates ABA signaling negatively and abiotic stress signaling positively.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, Dhaula Kuan, New Delhi, India.

ABSTRACT
Arabidopsis PP2C belonging to group A have been extensively worked out and known to negatively regulate ABA signaling. However, rice (Oryza sativa) orthologs of Arabidopsis group A PP2C are scarcely characterized functionally. We have identified a group A PP2C from rice (OsPP108), which is highly inducible under ABA, salt and drought stresses and localized predominantly in the nucleus. Genetic analysis revealed that Arabidopsis plants overexpressing OsPP108 are highly insensitive to ABA and tolerant to high salt and mannitol stresses during seed germination, root growth and overall seedling growth. At adult stage, OsPP108 overexpression leads to high tolerance to salt, mannitol and drought stresses with far better physiological parameters such as water loss, fresh weight, chlorophyll content and photosynthetic potential (Fv/Fm) in transgenic Arabidopsis plants. Expression profile of various stress marker genes in OsPP108 overexpressing plants revealed interplay of ABA dependent and independent pathway for abiotic stress tolerance. Overall, this study has identified a potential rice group A PP2C, which regulates ABA signaling negatively and abiotic stress signaling positively. Transgenic rice plants overexpressing this gene might provide an answer to the problem of low crop yield and productivity during adverse environmental conditions.

No MeSH data available.


Related in: MedlinePlus