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Urinary levels of high mobility group box-1 are associated with disease activity in antineutrophil cytoplasmic autoantibody-associated vasculitis.

Ma TT, Wang H, Wang C, Chang DY, Zhao MH, Chen M - PLoS ONE (2015)

Bottom Line: Our previous study demonstrated that the circulating levels of HMGB1 correlated with disease activity of antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV).Further analysis found that urinary levels of HMGB1 correlated with erythrocyte sedimentation rate (r=0.354, p=0.012), C-reactive protein (r=0.289, p=0.042), and Birmingham Vasculitis Activity Score (r=0.350, p=0.013).Urinary levels of HMGB1 may be associated with the disease activity in AAV patients.

View Article: PubMed Central - PubMed

Affiliation: Renal Division, Department of Medicine, Peking University First Hospital, Beijing, China.

ABSTRACT

Background: High mobility group box-1 (HMGB1), a kind of pro-inflammatory mediator, is associated with inflammatory conditions and tissue damage. Our previous study demonstrated that the circulating levels of HMGB1 correlated with disease activity of antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV). In the current study, we aimed to measure urinary levels of HMGB1 in AAV patients, correlated them to clinical activity index and analysed the immunohistochemical HMGB1 staining in kidney specimens.

Methods: 50 patients with AAV in active stage and 56 patients with AAV in remission were recruited. The urinary levels of HMGB1 were determined by enzyme-linked immunosorbent assay. Moreover, renal biopsy specimens from 27 patients with active AAV were randomly collected to evaluate the deposition of HMGB1.

Results: Urinary HMGB1 levels in AAV patients in active stage were significantly higher than those in AAV patients in remission and healthy controls (1.46 [0.56-3.43] versus 0.38 [0.10-1.35] mg/μmolCr, P=0.001; 1.46 [0.56-3.43] versus 0.48 [0.40-0.60] mg/μmolCr, P=0.000, respectively). Further analysis found that urinary levels of HMGB1 correlated with erythrocyte sedimentation rate (r=0.354, p=0.012), C-reactive protein (r=0.289, p=0.042), and Birmingham Vasculitis Activity Score (r=0.350, p=0.013). Renal tissue of active AAV patients showed HMGB1 was mainly expressed in the cytoplasm and the extracellular space. The percentage of HMGB1-negative nuclei in renal tissue of patients with active AAV was significantly higher than that in normal controls (60.6±20.2 % versus 2.7±0.6 %, p<0.01).

Conclusion: Urinary levels of HMGB1 may be associated with the disease activity in AAV patients.

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Changes of urinary HMGB1 levels in 19 AAV patients with sequential urinary samples.
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pone.0123586.g002: Changes of urinary HMGB1 levels in 19 AAV patients with sequential urinary samples.

Mentions: Urinary levels of HMGB1 in nineteen AAV patients with paired urinary samples of both active stage and remission were then compared. The urinary levels of HMGB1 were significantly lower in remission than those in active stage (median 1.38 [0.89–4.47] versus 0.40 [0.23–1.87] (mg/μmolCr), P = 0.011). Sixteen out of these nineteen patients had a decrease in urinary level of HMGB1 in remission compared with those in active stage. Only three patients had higher urinary levels of HMGB1 than in active stage; all these three patients were in complete remission, with BVAS of zero (Fig 2).


Urinary levels of high mobility group box-1 are associated with disease activity in antineutrophil cytoplasmic autoantibody-associated vasculitis.

Ma TT, Wang H, Wang C, Chang DY, Zhao MH, Chen M - PLoS ONE (2015)

Changes of urinary HMGB1 levels in 19 AAV patients with sequential urinary samples.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4401773&req=5

pone.0123586.g002: Changes of urinary HMGB1 levels in 19 AAV patients with sequential urinary samples.
Mentions: Urinary levels of HMGB1 in nineteen AAV patients with paired urinary samples of both active stage and remission were then compared. The urinary levels of HMGB1 were significantly lower in remission than those in active stage (median 1.38 [0.89–4.47] versus 0.40 [0.23–1.87] (mg/μmolCr), P = 0.011). Sixteen out of these nineteen patients had a decrease in urinary level of HMGB1 in remission compared with those in active stage. Only three patients had higher urinary levels of HMGB1 than in active stage; all these three patients were in complete remission, with BVAS of zero (Fig 2).

Bottom Line: Our previous study demonstrated that the circulating levels of HMGB1 correlated with disease activity of antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV).Further analysis found that urinary levels of HMGB1 correlated with erythrocyte sedimentation rate (r=0.354, p=0.012), C-reactive protein (r=0.289, p=0.042), and Birmingham Vasculitis Activity Score (r=0.350, p=0.013).Urinary levels of HMGB1 may be associated with the disease activity in AAV patients.

View Article: PubMed Central - PubMed

Affiliation: Renal Division, Department of Medicine, Peking University First Hospital, Beijing, China.

ABSTRACT

Background: High mobility group box-1 (HMGB1), a kind of pro-inflammatory mediator, is associated with inflammatory conditions and tissue damage. Our previous study demonstrated that the circulating levels of HMGB1 correlated with disease activity of antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV). In the current study, we aimed to measure urinary levels of HMGB1 in AAV patients, correlated them to clinical activity index and analysed the immunohistochemical HMGB1 staining in kidney specimens.

Methods: 50 patients with AAV in active stage and 56 patients with AAV in remission were recruited. The urinary levels of HMGB1 were determined by enzyme-linked immunosorbent assay. Moreover, renal biopsy specimens from 27 patients with active AAV were randomly collected to evaluate the deposition of HMGB1.

Results: Urinary HMGB1 levels in AAV patients in active stage were significantly higher than those in AAV patients in remission and healthy controls (1.46 [0.56-3.43] versus 0.38 [0.10-1.35] mg/μmolCr, P=0.001; 1.46 [0.56-3.43] versus 0.48 [0.40-0.60] mg/μmolCr, P=0.000, respectively). Further analysis found that urinary levels of HMGB1 correlated with erythrocyte sedimentation rate (r=0.354, p=0.012), C-reactive protein (r=0.289, p=0.042), and Birmingham Vasculitis Activity Score (r=0.350, p=0.013). Renal tissue of active AAV patients showed HMGB1 was mainly expressed in the cytoplasm and the extracellular space. The percentage of HMGB1-negative nuclei in renal tissue of patients with active AAV was significantly higher than that in normal controls (60.6±20.2 % versus 2.7±0.6 %, p<0.01).

Conclusion: Urinary levels of HMGB1 may be associated with the disease activity in AAV patients.

Show MeSH
Related in: MedlinePlus