Limits...
Analysis of Kif5b expression during mouse kidney development.

Cui J, Li X, Duan Z, Xue W, Wang Z, Lu S, Lin R, Liu M, Zhu G, Huang JD - PLoS ONE (2015)

Bottom Line: The distribution of Kif5b was analyzed by immunostaining.In kidneys of postnatal day 20 or of older mice, however, Kif5b was localized selectively in the basolateral domain of epithelial cells of the thick ascending loop of Henle, as well as of the distal convoluted tubule, with little expression being observed in the proximal tubule or in the collecting duct.Conditional knock-down of Kif5b in mouse kidney did not result in detectable morphological defects, but it did lead to a decrease in cell proliferation rate and also to a mislocalization of Na+/K+/-ATPase, indicating that although Kif5b is non-essential for kidney morphogenesis, it is important for nephron maturation.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Geriatrics, Beijing Hospital & Beijing Institute of Geriatrics, Ministry of Health, Beijing, China; Department of Biochemistry, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China.

ABSTRACT
Recent studies showed that kidney-specific inactivation of Kif3a produces kidney cysts and renal failure, suggesting that kinesin-mediated intracellular transportation is important for the establishement and maintenance of renal epithelial cell polarity and normal nephron functions. Kif5b, one of the most conserved kinesin heavy chain, is the mouse homologue of the human ubiquitous Kinesin Heavy Chain (uKHC). In order to elucidate the role of Kif5b in kidney development and function, it is essential to establish its expression profile within the organ. Therefore, in this study, we examined the expression pattern of Kif5b in mouse kidney. Kidneys from embryonic (E) 12.5-, 16.5-dpc (days post coitus) mouse fetuses, from postnatal (P) day 0, 10, 20 pups and from adult mice were collected. The distribution of Kif5b was analyzed by immunostaining. The possible involvement of Kif5b in kidney development was investigated in conditional mutant mice by using a Cre-LoxP strategy. This study showed that the distribution of Kif5b displayed spatiotemporal changes during postnatal kidney development. In kidneys of new born mice, Kif5b was strongly expressed in all developing tubules and in the ureteric bud, but not in the glomerulus or in other early-developing structures, such as the cap mesenchyme, the comma-shaped body, and the S-shaped body. In kidneys of postnatal day 20 or of older mice, however, Kif5b was localized selectively in the basolateral domain of epithelial cells of the thick ascending loop of Henle, as well as of the distal convoluted tubule, with little expression being observed in the proximal tubule or in the collecting duct. Conditional knock-down of Kif5b in mouse kidney did not result in detectable morphological defects, but it did lead to a decrease in cell proliferation rate and also to a mislocalization of Na+/K+/-ATPase, indicating that although Kif5b is non-essential for kidney morphogenesis, it is important for nephron maturation.

No MeSH data available.


Related in: MedlinePlus

Expression pattern of Kif5b in newborn mouse kidney.(A) Overall view of Kif5b expression in the kidney: strong expression in tubular structures in both cortex and medulla, and low levels of expression in the nephrogenic zone. (B) In the nephrogenic zone, Kif5b was expressed in the ureteric bud, but exhibited a very low expression in the cap mesenchyme, comma-shaped body, S-shaped body and advanced S-shaped body. (C, D) Kif5b was widely expressed in the renal tubules of the cortex and the medulla, but expression in glomeruli and in interstitial cells was quite rare. G, glomerulus; PCT, proximal convoluted tubules; TAL, thick ascending limbs of loops of Henle; DCT, distal convoluted tubule; CD, collecting duct. Images are representative of kidney tissue sections from five mice. Scale bar: (A) 500 μm, (B-D) 50 μm.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4401754&req=5

pone.0126002.g001: Expression pattern of Kif5b in newborn mouse kidney.(A) Overall view of Kif5b expression in the kidney: strong expression in tubular structures in both cortex and medulla, and low levels of expression in the nephrogenic zone. (B) In the nephrogenic zone, Kif5b was expressed in the ureteric bud, but exhibited a very low expression in the cap mesenchyme, comma-shaped body, S-shaped body and advanced S-shaped body. (C, D) Kif5b was widely expressed in the renal tubules of the cortex and the medulla, but expression in glomeruli and in interstitial cells was quite rare. G, glomerulus; PCT, proximal convoluted tubules; TAL, thick ascending limbs of loops of Henle; DCT, distal convoluted tubule; CD, collecting duct. Images are representative of kidney tissue sections from five mice. Scale bar: (A) 500 μm, (B-D) 50 μm.

Mentions: To characterize Kif5b expression in the kidney, we analyzed the distribution of Kif5b in neonatal kidneys. In the newborn mouse, the kidney can be divided into three parts: the outermost part is the nephrogenic zone where nephrogenesis occurs, and the inner parts are the cortex and the medulla, respectively (Fig 1A). Kidney cortex is composed of glomeruli and renal tubules, while the medulla comprises only tubules. The interstitium is filled among the parenchyma in the whole kidney.


Analysis of Kif5b expression during mouse kidney development.

Cui J, Li X, Duan Z, Xue W, Wang Z, Lu S, Lin R, Liu M, Zhu G, Huang JD - PLoS ONE (2015)

Expression pattern of Kif5b in newborn mouse kidney.(A) Overall view of Kif5b expression in the kidney: strong expression in tubular structures in both cortex and medulla, and low levels of expression in the nephrogenic zone. (B) In the nephrogenic zone, Kif5b was expressed in the ureteric bud, but exhibited a very low expression in the cap mesenchyme, comma-shaped body, S-shaped body and advanced S-shaped body. (C, D) Kif5b was widely expressed in the renal tubules of the cortex and the medulla, but expression in glomeruli and in interstitial cells was quite rare. G, glomerulus; PCT, proximal convoluted tubules; TAL, thick ascending limbs of loops of Henle; DCT, distal convoluted tubule; CD, collecting duct. Images are representative of kidney tissue sections from five mice. Scale bar: (A) 500 μm, (B-D) 50 μm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4401754&req=5

pone.0126002.g001: Expression pattern of Kif5b in newborn mouse kidney.(A) Overall view of Kif5b expression in the kidney: strong expression in tubular structures in both cortex and medulla, and low levels of expression in the nephrogenic zone. (B) In the nephrogenic zone, Kif5b was expressed in the ureteric bud, but exhibited a very low expression in the cap mesenchyme, comma-shaped body, S-shaped body and advanced S-shaped body. (C, D) Kif5b was widely expressed in the renal tubules of the cortex and the medulla, but expression in glomeruli and in interstitial cells was quite rare. G, glomerulus; PCT, proximal convoluted tubules; TAL, thick ascending limbs of loops of Henle; DCT, distal convoluted tubule; CD, collecting duct. Images are representative of kidney tissue sections from five mice. Scale bar: (A) 500 μm, (B-D) 50 μm.
Mentions: To characterize Kif5b expression in the kidney, we analyzed the distribution of Kif5b in neonatal kidneys. In the newborn mouse, the kidney can be divided into three parts: the outermost part is the nephrogenic zone where nephrogenesis occurs, and the inner parts are the cortex and the medulla, respectively (Fig 1A). Kidney cortex is composed of glomeruli and renal tubules, while the medulla comprises only tubules. The interstitium is filled among the parenchyma in the whole kidney.

Bottom Line: The distribution of Kif5b was analyzed by immunostaining.In kidneys of postnatal day 20 or of older mice, however, Kif5b was localized selectively in the basolateral domain of epithelial cells of the thick ascending loop of Henle, as well as of the distal convoluted tubule, with little expression being observed in the proximal tubule or in the collecting duct.Conditional knock-down of Kif5b in mouse kidney did not result in detectable morphological defects, but it did lead to a decrease in cell proliferation rate and also to a mislocalization of Na+/K+/-ATPase, indicating that although Kif5b is non-essential for kidney morphogenesis, it is important for nephron maturation.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Geriatrics, Beijing Hospital & Beijing Institute of Geriatrics, Ministry of Health, Beijing, China; Department of Biochemistry, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China.

ABSTRACT
Recent studies showed that kidney-specific inactivation of Kif3a produces kidney cysts and renal failure, suggesting that kinesin-mediated intracellular transportation is important for the establishement and maintenance of renal epithelial cell polarity and normal nephron functions. Kif5b, one of the most conserved kinesin heavy chain, is the mouse homologue of the human ubiquitous Kinesin Heavy Chain (uKHC). In order to elucidate the role of Kif5b in kidney development and function, it is essential to establish its expression profile within the organ. Therefore, in this study, we examined the expression pattern of Kif5b in mouse kidney. Kidneys from embryonic (E) 12.5-, 16.5-dpc (days post coitus) mouse fetuses, from postnatal (P) day 0, 10, 20 pups and from adult mice were collected. The distribution of Kif5b was analyzed by immunostaining. The possible involvement of Kif5b in kidney development was investigated in conditional mutant mice by using a Cre-LoxP strategy. This study showed that the distribution of Kif5b displayed spatiotemporal changes during postnatal kidney development. In kidneys of new born mice, Kif5b was strongly expressed in all developing tubules and in the ureteric bud, but not in the glomerulus or in other early-developing structures, such as the cap mesenchyme, the comma-shaped body, and the S-shaped body. In kidneys of postnatal day 20 or of older mice, however, Kif5b was localized selectively in the basolateral domain of epithelial cells of the thick ascending loop of Henle, as well as of the distal convoluted tubule, with little expression being observed in the proximal tubule or in the collecting duct. Conditional knock-down of Kif5b in mouse kidney did not result in detectable morphological defects, but it did lead to a decrease in cell proliferation rate and also to a mislocalization of Na+/K+/-ATPase, indicating that although Kif5b is non-essential for kidney morphogenesis, it is important for nephron maturation.

No MeSH data available.


Related in: MedlinePlus