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Thermochemical ablation therapy of VX2 tumor using a permeable oil-packed liquid alkali metal.

Guo Z, Zhang Q, Li X, Jing Z - PLoS ONE (2015)

Bottom Line: The injection of the NaK-oil mixture resulted in significantly higher heat in the ablation areas.Perfusion computed tomography and its parameter map confirmed that the NaK-oil mixture had curative effects on VX2 tumors.With reference to the controlled thermal and chemical lethal injury to tumors, using a liquid alkali in ablation is potentially an effective and safe method to treat malignant tumors.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China; Department of Radiology, Haikou People's Hospital, Xiangya Medical School Central South University, Haikou City, Hainan, China.

ABSTRACT

Objective: Alkali metal appears to be a promising tool in thermochemical ablation, but, it requires additional data on safety is required. The objective of this study was to explore the effectiveness of permeable oil-packed liquid alkali metal in the thermochemical ablation of tumors.

Methods: Permeable oil-packed sodium-potassium (NaK) was prepared using ultrasonic mixing of different ratios of metal to oil. The thermal effect of the mixture during ablation of muscle tissue ex vivo was evaluated using the Fluke Ti400 Thermal Imager. The thermochemical effect of the NaK-oil mixture on VX2 tumors was evaluated by performing perfusion CT scans both before and after treatment in 10 VX2 rabbit model tumors. VX2 tumors were harvested from two rabbits immediately after treatment to assess their viability using trypan blue and hematoxylin and eosin (H.E.) staining.

Results: The injection of the NaK-oil mixture resulted in significantly higher heat in the ablation areas. The permeable oil controlled the rate of heat released during the NaK reaction with water in the living tissue. Perfusion computed tomography and its parameter map confirmed that the NaK-oil mixture had curative effects on VX2 tumors. Both trypan blue and H.E. staining showed partial necrosis of the VX2 tumors.

Conclusions: The NaK-oil mixture may be used successfully to ablate tumor tissue in vivo. With reference to the controlled thermal and chemical lethal injury to tumors, using a liquid alkali in ablation is potentially an effective and safe method to treat malignant tumors.

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Related in: MedlinePlus

Histopathology analysis of the VX2 tumor.A) Viable cells exclude Trypan blue (TB), while dead cells stain blue from TB uptake. B) Region near the corners (star points) demonstrates areas with integrated tumor structure, while hexagram shows hemorrhagic necrosis areas, X 100. C) Region demonstrates nucleus of tumor cells was completely dissolved and necrotic, X 100. D) Region near corners (star points) demonstrates integrated tumor structure, while hexagram shows hemorrhagic necrotic areas, X 200. E) Regions demonstrate complete karyolysis of tumor cells and a large amount of red blood cell leakage, X 400. TB staining of VX2 tumor after treatment with NaK–oil mixture in vivo shows damage to the tissue structure of tumor, which had been further verified by pathology Hematoxylin/eosin staining.
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pone.0123196.g005: Histopathology analysis of the VX2 tumor.A) Viable cells exclude Trypan blue (TB), while dead cells stain blue from TB uptake. B) Region near the corners (star points) demonstrates areas with integrated tumor structure, while hexagram shows hemorrhagic necrosis areas, X 100. C) Region demonstrates nucleus of tumor cells was completely dissolved and necrotic, X 100. D) Region near corners (star points) demonstrates integrated tumor structure, while hexagram shows hemorrhagic necrotic areas, X 200. E) Regions demonstrate complete karyolysis of tumor cells and a large amount of red blood cell leakage, X 400. TB staining of VX2 tumor after treatment with NaK–oil mixture in vivo shows damage to the tissue structure of tumor, which had been further verified by pathology Hematoxylin/eosin staining.

Mentions: The NaK–oil ablation showed typical signs of coagulative necrosis, including pyknotic nuclei and streamlined cytoplasm (Fig 5). There was vascular thrombosis and hemorrhagic changes in the cells and/or necrosis, as shown in Fig 5B–5E. The VX2 tumor treated with an extremely small amount of NaK–oil mixture shows evidence of cell death and necrosis. These results indicated that permeable oil-controlled release of Na/K could be effective in the treatment of treat tumors.


Thermochemical ablation therapy of VX2 tumor using a permeable oil-packed liquid alkali metal.

Guo Z, Zhang Q, Li X, Jing Z - PLoS ONE (2015)

Histopathology analysis of the VX2 tumor.A) Viable cells exclude Trypan blue (TB), while dead cells stain blue from TB uptake. B) Region near the corners (star points) demonstrates areas with integrated tumor structure, while hexagram shows hemorrhagic necrosis areas, X 100. C) Region demonstrates nucleus of tumor cells was completely dissolved and necrotic, X 100. D) Region near corners (star points) demonstrates integrated tumor structure, while hexagram shows hemorrhagic necrotic areas, X 200. E) Regions demonstrate complete karyolysis of tumor cells and a large amount of red blood cell leakage, X 400. TB staining of VX2 tumor after treatment with NaK–oil mixture in vivo shows damage to the tissue structure of tumor, which had been further verified by pathology Hematoxylin/eosin staining.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4401739&req=5

pone.0123196.g005: Histopathology analysis of the VX2 tumor.A) Viable cells exclude Trypan blue (TB), while dead cells stain blue from TB uptake. B) Region near the corners (star points) demonstrates areas with integrated tumor structure, while hexagram shows hemorrhagic necrosis areas, X 100. C) Region demonstrates nucleus of tumor cells was completely dissolved and necrotic, X 100. D) Region near corners (star points) demonstrates integrated tumor structure, while hexagram shows hemorrhagic necrotic areas, X 200. E) Regions demonstrate complete karyolysis of tumor cells and a large amount of red blood cell leakage, X 400. TB staining of VX2 tumor after treatment with NaK–oil mixture in vivo shows damage to the tissue structure of tumor, which had been further verified by pathology Hematoxylin/eosin staining.
Mentions: The NaK–oil ablation showed typical signs of coagulative necrosis, including pyknotic nuclei and streamlined cytoplasm (Fig 5). There was vascular thrombosis and hemorrhagic changes in the cells and/or necrosis, as shown in Fig 5B–5E. The VX2 tumor treated with an extremely small amount of NaK–oil mixture shows evidence of cell death and necrosis. These results indicated that permeable oil-controlled release of Na/K could be effective in the treatment of treat tumors.

Bottom Line: The injection of the NaK-oil mixture resulted in significantly higher heat in the ablation areas.Perfusion computed tomography and its parameter map confirmed that the NaK-oil mixture had curative effects on VX2 tumors.With reference to the controlled thermal and chemical lethal injury to tumors, using a liquid alkali in ablation is potentially an effective and safe method to treat malignant tumors.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China; Department of Radiology, Haikou People's Hospital, Xiangya Medical School Central South University, Haikou City, Hainan, China.

ABSTRACT

Objective: Alkali metal appears to be a promising tool in thermochemical ablation, but, it requires additional data on safety is required. The objective of this study was to explore the effectiveness of permeable oil-packed liquid alkali metal in the thermochemical ablation of tumors.

Methods: Permeable oil-packed sodium-potassium (NaK) was prepared using ultrasonic mixing of different ratios of metal to oil. The thermal effect of the mixture during ablation of muscle tissue ex vivo was evaluated using the Fluke Ti400 Thermal Imager. The thermochemical effect of the NaK-oil mixture on VX2 tumors was evaluated by performing perfusion CT scans both before and after treatment in 10 VX2 rabbit model tumors. VX2 tumors were harvested from two rabbits immediately after treatment to assess their viability using trypan blue and hematoxylin and eosin (H.E.) staining.

Results: The injection of the NaK-oil mixture resulted in significantly higher heat in the ablation areas. The permeable oil controlled the rate of heat released during the NaK reaction with water in the living tissue. Perfusion computed tomography and its parameter map confirmed that the NaK-oil mixture had curative effects on VX2 tumors. Both trypan blue and H.E. staining showed partial necrosis of the VX2 tumors.

Conclusions: The NaK-oil mixture may be used successfully to ablate tumor tissue in vivo. With reference to the controlled thermal and chemical lethal injury to tumors, using a liquid alkali in ablation is potentially an effective and safe method to treat malignant tumors.

Show MeSH
Related in: MedlinePlus