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Thermochemical ablation therapy of VX2 tumor using a permeable oil-packed liquid alkali metal.

Guo Z, Zhang Q, Li X, Jing Z - PLoS ONE (2015)

Bottom Line: The injection of the NaK-oil mixture resulted in significantly higher heat in the ablation areas.Perfusion computed tomography and its parameter map confirmed that the NaK-oil mixture had curative effects on VX2 tumors.With reference to the controlled thermal and chemical lethal injury to tumors, using a liquid alkali in ablation is potentially an effective and safe method to treat malignant tumors.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China; Department of Radiology, Haikou People's Hospital, Xiangya Medical School Central South University, Haikou City, Hainan, China.

ABSTRACT

Objective: Alkali metal appears to be a promising tool in thermochemical ablation, but, it requires additional data on safety is required. The objective of this study was to explore the effectiveness of permeable oil-packed liquid alkali metal in the thermochemical ablation of tumors.

Methods: Permeable oil-packed sodium-potassium (NaK) was prepared using ultrasonic mixing of different ratios of metal to oil. The thermal effect of the mixture during ablation of muscle tissue ex vivo was evaluated using the Fluke Ti400 Thermal Imager. The thermochemical effect of the NaK-oil mixture on VX2 tumors was evaluated by performing perfusion CT scans both before and after treatment in 10 VX2 rabbit model tumors. VX2 tumors were harvested from two rabbits immediately after treatment to assess their viability using trypan blue and hematoxylin and eosin (H.E.) staining.

Results: The injection of the NaK-oil mixture resulted in significantly higher heat in the ablation areas. The permeable oil controlled the rate of heat released during the NaK reaction with water in the living tissue. Perfusion computed tomography and its parameter map confirmed that the NaK-oil mixture had curative effects on VX2 tumors. Both trypan blue and H.E. staining showed partial necrosis of the VX2 tumors.

Conclusions: The NaK-oil mixture may be used successfully to ablate tumor tissue in vivo. With reference to the controlled thermal and chemical lethal injury to tumors, using a liquid alkali in ablation is potentially an effective and safe method to treat malignant tumors.

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Related in: MedlinePlus

Axial CT images in rabbits show normal muscle tissue and VX2 tumor (black arrow): (A, B) before and (C, D) after NaK–oil mixture (10 μL:50 μL, vol:vol) injection.Baseline images were obtained 15.3 sec (A) and 25.4 sec (B) after the start of contrast material injection, and perfusion images were obtained 15.3 sec (C) and 25.4 sec (D) seconds after start of the NaK–oil mixture injection. In C and D, note the reduced contrast enhancement of the tumor, especially its outer rim. The reaction between NaK and water in the tissue produces a large amount of air (white dovetail) during the exothermic chemical process (C and D).
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pone.0123196.g004: Axial CT images in rabbits show normal muscle tissue and VX2 tumor (black arrow): (A, B) before and (C, D) after NaK–oil mixture (10 μL:50 μL, vol:vol) injection.Baseline images were obtained 15.3 sec (A) and 25.4 sec (B) after the start of contrast material injection, and perfusion images were obtained 15.3 sec (C) and 25.4 sec (D) seconds after start of the NaK–oil mixture injection. In C and D, note the reduced contrast enhancement of the tumor, especially its outer rim. The reaction between NaK and water in the tissue produces a large amount of air (white dovetail) during the exothermic chemical process (C and D).

Mentions: Both plain and perfusion CT scans were used to differentiate the necrosis area from the viable area to evaluate the curative effects of the NaK-oil ablation treatment for the VX2 tumor in vivo. The post-perfusion CT examinations were performed after an interval of >1.0 hour after the first CT. The gas density displayed by CT scan indicated the area where the reaction of NaK with water occurred in the tissues. Comparing perfusion CT before and after treatment, the post-treatment CT showed significantly reduced BF, BV, and permeability of the tumor, which was lowest at the center of the ablated lesion (Fig 4, Table 1).


Thermochemical ablation therapy of VX2 tumor using a permeable oil-packed liquid alkali metal.

Guo Z, Zhang Q, Li X, Jing Z - PLoS ONE (2015)

Axial CT images in rabbits show normal muscle tissue and VX2 tumor (black arrow): (A, B) before and (C, D) after NaK–oil mixture (10 μL:50 μL, vol:vol) injection.Baseline images were obtained 15.3 sec (A) and 25.4 sec (B) after the start of contrast material injection, and perfusion images were obtained 15.3 sec (C) and 25.4 sec (D) seconds after start of the NaK–oil mixture injection. In C and D, note the reduced contrast enhancement of the tumor, especially its outer rim. The reaction between NaK and water in the tissue produces a large amount of air (white dovetail) during the exothermic chemical process (C and D).
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4401739&req=5

pone.0123196.g004: Axial CT images in rabbits show normal muscle tissue and VX2 tumor (black arrow): (A, B) before and (C, D) after NaK–oil mixture (10 μL:50 μL, vol:vol) injection.Baseline images were obtained 15.3 sec (A) and 25.4 sec (B) after the start of contrast material injection, and perfusion images were obtained 15.3 sec (C) and 25.4 sec (D) seconds after start of the NaK–oil mixture injection. In C and D, note the reduced contrast enhancement of the tumor, especially its outer rim. The reaction between NaK and water in the tissue produces a large amount of air (white dovetail) during the exothermic chemical process (C and D).
Mentions: Both plain and perfusion CT scans were used to differentiate the necrosis area from the viable area to evaluate the curative effects of the NaK-oil ablation treatment for the VX2 tumor in vivo. The post-perfusion CT examinations were performed after an interval of >1.0 hour after the first CT. The gas density displayed by CT scan indicated the area where the reaction of NaK with water occurred in the tissues. Comparing perfusion CT before and after treatment, the post-treatment CT showed significantly reduced BF, BV, and permeability of the tumor, which was lowest at the center of the ablated lesion (Fig 4, Table 1).

Bottom Line: The injection of the NaK-oil mixture resulted in significantly higher heat in the ablation areas.Perfusion computed tomography and its parameter map confirmed that the NaK-oil mixture had curative effects on VX2 tumors.With reference to the controlled thermal and chemical lethal injury to tumors, using a liquid alkali in ablation is potentially an effective and safe method to treat malignant tumors.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China; Department of Radiology, Haikou People's Hospital, Xiangya Medical School Central South University, Haikou City, Hainan, China.

ABSTRACT

Objective: Alkali metal appears to be a promising tool in thermochemical ablation, but, it requires additional data on safety is required. The objective of this study was to explore the effectiveness of permeable oil-packed liquid alkali metal in the thermochemical ablation of tumors.

Methods: Permeable oil-packed sodium-potassium (NaK) was prepared using ultrasonic mixing of different ratios of metal to oil. The thermal effect of the mixture during ablation of muscle tissue ex vivo was evaluated using the Fluke Ti400 Thermal Imager. The thermochemical effect of the NaK-oil mixture on VX2 tumors was evaluated by performing perfusion CT scans both before and after treatment in 10 VX2 rabbit model tumors. VX2 tumors were harvested from two rabbits immediately after treatment to assess their viability using trypan blue and hematoxylin and eosin (H.E.) staining.

Results: The injection of the NaK-oil mixture resulted in significantly higher heat in the ablation areas. The permeable oil controlled the rate of heat released during the NaK reaction with water in the living tissue. Perfusion computed tomography and its parameter map confirmed that the NaK-oil mixture had curative effects on VX2 tumors. Both trypan blue and H.E. staining showed partial necrosis of the VX2 tumors.

Conclusions: The NaK-oil mixture may be used successfully to ablate tumor tissue in vivo. With reference to the controlled thermal and chemical lethal injury to tumors, using a liquid alkali in ablation is potentially an effective and safe method to treat malignant tumors.

Show MeSH
Related in: MedlinePlus