Limits...
Thermochemical ablation therapy of VX2 tumor using a permeable oil-packed liquid alkali metal.

Guo Z, Zhang Q, Li X, Jing Z - PLoS ONE (2015)

Bottom Line: The injection of the NaK-oil mixture resulted in significantly higher heat in the ablation areas.Perfusion computed tomography and its parameter map confirmed that the NaK-oil mixture had curative effects on VX2 tumors.With reference to the controlled thermal and chemical lethal injury to tumors, using a liquid alkali in ablation is potentially an effective and safe method to treat malignant tumors.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China; Department of Radiology, Haikou People's Hospital, Xiangya Medical School Central South University, Haikou City, Hainan, China.

ABSTRACT

Objective: Alkali metal appears to be a promising tool in thermochemical ablation, but, it requires additional data on safety is required. The objective of this study was to explore the effectiveness of permeable oil-packed liquid alkali metal in the thermochemical ablation of tumors.

Methods: Permeable oil-packed sodium-potassium (NaK) was prepared using ultrasonic mixing of different ratios of metal to oil. The thermal effect of the mixture during ablation of muscle tissue ex vivo was evaluated using the Fluke Ti400 Thermal Imager. The thermochemical effect of the NaK-oil mixture on VX2 tumors was evaluated by performing perfusion CT scans both before and after treatment in 10 VX2 rabbit model tumors. VX2 tumors were harvested from two rabbits immediately after treatment to assess their viability using trypan blue and hematoxylin and eosin (H.E.) staining.

Results: The injection of the NaK-oil mixture resulted in significantly higher heat in the ablation areas. The permeable oil controlled the rate of heat released during the NaK reaction with water in the living tissue. Perfusion computed tomography and its parameter map confirmed that the NaK-oil mixture had curative effects on VX2 tumors. Both trypan blue and H.E. staining showed partial necrosis of the VX2 tumors.

Conclusions: The NaK-oil mixture may be used successfully to ablate tumor tissue in vivo. With reference to the controlled thermal and chemical lethal injury to tumors, using a liquid alkali in ablation is potentially an effective and safe method to treat malignant tumors.

Show MeSH

Related in: MedlinePlus

Temperature changes in spatial and temperal relationships during the injection of sodium-potassium alloy-oil mixture.Ai and Aii) Video stream of the thermal damage as acquired by the infrared Fluke Ti400 Thermal Imager. A0 to A2 indicates the temperature distribution (Ai) and alteration at different distances from the reaction center (Aii). The continuous region of interest selection A0, A1, A2, points in the tissue that the heat readings, and the distance from the center heat source are approximately 0mm (A0), 1mm (A1), 2mm (A2). Bi, Bii, Biii) Temperature to time curves were captured from three muscle strips corresponding to different ratios of a mixture of NaK and oil 1:10 (Bi), 1:5(Bii), and 1:2 (Biii) for the ablation of muscle with the same amount of NaK. C) From left to right, three muscle strips corresponding to different ratios of a mixture of NaK and oil (1:10, 1:5, and 1:2) for the ablation of muscle. Coming to each muscle stripe, three seperate ablation points with the same volume of the mixture. D) Effects of the NaK–oil mixture on muscle tissue and cells: apparent necrosis of a large number of muscle cells with the disappearance of cell structure and dissolved nuclei.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4401739&req=5

pone.0123196.g003: Temperature changes in spatial and temperal relationships during the injection of sodium-potassium alloy-oil mixture.Ai and Aii) Video stream of the thermal damage as acquired by the infrared Fluke Ti400 Thermal Imager. A0 to A2 indicates the temperature distribution (Ai) and alteration at different distances from the reaction center (Aii). The continuous region of interest selection A0, A1, A2, points in the tissue that the heat readings, and the distance from the center heat source are approximately 0mm (A0), 1mm (A1), 2mm (A2). Bi, Bii, Biii) Temperature to time curves were captured from three muscle strips corresponding to different ratios of a mixture of NaK and oil 1:10 (Bi), 1:5(Bii), and 1:2 (Biii) for the ablation of muscle with the same amount of NaK. C) From left to right, three muscle strips corresponding to different ratios of a mixture of NaK and oil (1:10, 1:5, and 1:2) for the ablation of muscle. Coming to each muscle stripe, three seperate ablation points with the same volume of the mixture. D) Effects of the NaK–oil mixture on muscle tissue and cells: apparent necrosis of a large number of muscle cells with the disappearance of cell structure and dissolved nuclei.

Mentions: Temperature changes in spatial and temporal relationships are presented in Fig 3A and 3B. The injection of the NaK–oil mixture resulted in significantly increase in temperature in the ablation areas from 18.93 to 35.90°C (Tmax−Tmin = 17°C). The instantaneous temperature had been above 75°C at center position; with increasing distance from the center of the reaction, the temperature was lower. The rising slopes and amplitude of the temperature in the ablation area were similar among different ratios of NaK to oil. The most significant rise in temperature was observed within the first 1.0-minute interval, as shown in Fig 3B. When comparing the differences in temperature mapping among the three ratios of NaK to oil, a higher NaK-to-oil ratio (e.g., 1:2) corresponded to a thinner peak of temperature alterations and shorter duration.


Thermochemical ablation therapy of VX2 tumor using a permeable oil-packed liquid alkali metal.

Guo Z, Zhang Q, Li X, Jing Z - PLoS ONE (2015)

Temperature changes in spatial and temperal relationships during the injection of sodium-potassium alloy-oil mixture.Ai and Aii) Video stream of the thermal damage as acquired by the infrared Fluke Ti400 Thermal Imager. A0 to A2 indicates the temperature distribution (Ai) and alteration at different distances from the reaction center (Aii). The continuous region of interest selection A0, A1, A2, points in the tissue that the heat readings, and the distance from the center heat source are approximately 0mm (A0), 1mm (A1), 2mm (A2). Bi, Bii, Biii) Temperature to time curves were captured from three muscle strips corresponding to different ratios of a mixture of NaK and oil 1:10 (Bi), 1:5(Bii), and 1:2 (Biii) for the ablation of muscle with the same amount of NaK. C) From left to right, three muscle strips corresponding to different ratios of a mixture of NaK and oil (1:10, 1:5, and 1:2) for the ablation of muscle. Coming to each muscle stripe, three seperate ablation points with the same volume of the mixture. D) Effects of the NaK–oil mixture on muscle tissue and cells: apparent necrosis of a large number of muscle cells with the disappearance of cell structure and dissolved nuclei.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4401739&req=5

pone.0123196.g003: Temperature changes in spatial and temperal relationships during the injection of sodium-potassium alloy-oil mixture.Ai and Aii) Video stream of the thermal damage as acquired by the infrared Fluke Ti400 Thermal Imager. A0 to A2 indicates the temperature distribution (Ai) and alteration at different distances from the reaction center (Aii). The continuous region of interest selection A0, A1, A2, points in the tissue that the heat readings, and the distance from the center heat source are approximately 0mm (A0), 1mm (A1), 2mm (A2). Bi, Bii, Biii) Temperature to time curves were captured from three muscle strips corresponding to different ratios of a mixture of NaK and oil 1:10 (Bi), 1:5(Bii), and 1:2 (Biii) for the ablation of muscle with the same amount of NaK. C) From left to right, three muscle strips corresponding to different ratios of a mixture of NaK and oil (1:10, 1:5, and 1:2) for the ablation of muscle. Coming to each muscle stripe, three seperate ablation points with the same volume of the mixture. D) Effects of the NaK–oil mixture on muscle tissue and cells: apparent necrosis of a large number of muscle cells with the disappearance of cell structure and dissolved nuclei.
Mentions: Temperature changes in spatial and temporal relationships are presented in Fig 3A and 3B. The injection of the NaK–oil mixture resulted in significantly increase in temperature in the ablation areas from 18.93 to 35.90°C (Tmax−Tmin = 17°C). The instantaneous temperature had been above 75°C at center position; with increasing distance from the center of the reaction, the temperature was lower. The rising slopes and amplitude of the temperature in the ablation area were similar among different ratios of NaK to oil. The most significant rise in temperature was observed within the first 1.0-minute interval, as shown in Fig 3B. When comparing the differences in temperature mapping among the three ratios of NaK to oil, a higher NaK-to-oil ratio (e.g., 1:2) corresponded to a thinner peak of temperature alterations and shorter duration.

Bottom Line: The injection of the NaK-oil mixture resulted in significantly higher heat in the ablation areas.Perfusion computed tomography and its parameter map confirmed that the NaK-oil mixture had curative effects on VX2 tumors.With reference to the controlled thermal and chemical lethal injury to tumors, using a liquid alkali in ablation is potentially an effective and safe method to treat malignant tumors.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China; Department of Radiology, Haikou People's Hospital, Xiangya Medical School Central South University, Haikou City, Hainan, China.

ABSTRACT

Objective: Alkali metal appears to be a promising tool in thermochemical ablation, but, it requires additional data on safety is required. The objective of this study was to explore the effectiveness of permeable oil-packed liquid alkali metal in the thermochemical ablation of tumors.

Methods: Permeable oil-packed sodium-potassium (NaK) was prepared using ultrasonic mixing of different ratios of metal to oil. The thermal effect of the mixture during ablation of muscle tissue ex vivo was evaluated using the Fluke Ti400 Thermal Imager. The thermochemical effect of the NaK-oil mixture on VX2 tumors was evaluated by performing perfusion CT scans both before and after treatment in 10 VX2 rabbit model tumors. VX2 tumors were harvested from two rabbits immediately after treatment to assess their viability using trypan blue and hematoxylin and eosin (H.E.) staining.

Results: The injection of the NaK-oil mixture resulted in significantly higher heat in the ablation areas. The permeable oil controlled the rate of heat released during the NaK reaction with water in the living tissue. Perfusion computed tomography and its parameter map confirmed that the NaK-oil mixture had curative effects on VX2 tumors. Both trypan blue and H.E. staining showed partial necrosis of the VX2 tumors.

Conclusions: The NaK-oil mixture may be used successfully to ablate tumor tissue in vivo. With reference to the controlled thermal and chemical lethal injury to tumors, using a liquid alkali in ablation is potentially an effective and safe method to treat malignant tumors.

Show MeSH
Related in: MedlinePlus