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Thermochemical ablation therapy of VX2 tumor using a permeable oil-packed liquid alkali metal.

Guo Z, Zhang Q, Li X, Jing Z - PLoS ONE (2015)

Bottom Line: The injection of the NaK-oil mixture resulted in significantly higher heat in the ablation areas.Perfusion computed tomography and its parameter map confirmed that the NaK-oil mixture had curative effects on VX2 tumors.With reference to the controlled thermal and chemical lethal injury to tumors, using a liquid alkali in ablation is potentially an effective and safe method to treat malignant tumors.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China; Department of Radiology, Haikou People's Hospital, Xiangya Medical School Central South University, Haikou City, Hainan, China.

ABSTRACT

Objective: Alkali metal appears to be a promising tool in thermochemical ablation, but, it requires additional data on safety is required. The objective of this study was to explore the effectiveness of permeable oil-packed liquid alkali metal in the thermochemical ablation of tumors.

Methods: Permeable oil-packed sodium-potassium (NaK) was prepared using ultrasonic mixing of different ratios of metal to oil. The thermal effect of the mixture during ablation of muscle tissue ex vivo was evaluated using the Fluke Ti400 Thermal Imager. The thermochemical effect of the NaK-oil mixture on VX2 tumors was evaluated by performing perfusion CT scans both before and after treatment in 10 VX2 rabbit model tumors. VX2 tumors were harvested from two rabbits immediately after treatment to assess their viability using trypan blue and hematoxylin and eosin (H.E.) staining.

Results: The injection of the NaK-oil mixture resulted in significantly higher heat in the ablation areas. The permeable oil controlled the rate of heat released during the NaK reaction with water in the living tissue. Perfusion computed tomography and its parameter map confirmed that the NaK-oil mixture had curative effects on VX2 tumors. Both trypan blue and H.E. staining showed partial necrosis of the VX2 tumors.

Conclusions: The NaK-oil mixture may be used successfully to ablate tumor tissue in vivo. With reference to the controlled thermal and chemical lethal injury to tumors, using a liquid alkali in ablation is potentially an effective and safe method to treat malignant tumors.

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Related in: MedlinePlus

Axial perfusion CT image and time density curve corresponding to the regions of interest: center of the aorta as an arterial input reference (A), around entire tumor (T), around an area of muscle tissue immediately adjacent to the tumor (M), and random location in the normal muscle tissue distant from the tumor (N).
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pone.0123196.g002: Axial perfusion CT image and time density curve corresponding to the regions of interest: center of the aorta as an arterial input reference (A), around entire tumor (T), around an area of muscle tissue immediately adjacent to the tumor (M), and random location in the normal muscle tissue distant from the tumor (N).

Mentions: The perfusion images were reconstructed from the raw projection data onto a 512 × 512-pixel matrix before being transferred to a syngo.via imaging workstation (Siemens Healthcare) for perfusion analysis. Absolute values of blood flow (BF), blood volume (BV), and capillary permeability-surface area (PMB) measurements were determined using CT Body Tumor Perfusion (Siemens Healthcare). At each of the four simultaneously imaged levels, three ROIs were drawn around the entire tumor (T), around an area of normal tissue immediately adjacent to the tumor (M), and in a random location within the normal muscle tissue (N) distant from the tumor as shown in Fig 2. Careful placement of arterial input function region (A) is the basis for accurate determination of CT Perfusion Map Construction, as shown in Fig 2. The BF, BV, and PMB measurements were calculated in each of the three ROIs (T, M, N). All ROIs and perfusion measurements were drawn and obtained by the same investigator. The characteristics of enhancement deduced from the perfusion CT were analyzed offline. All the differences before and after ablation were compared to confirm any recorded changes.


Thermochemical ablation therapy of VX2 tumor using a permeable oil-packed liquid alkali metal.

Guo Z, Zhang Q, Li X, Jing Z - PLoS ONE (2015)

Axial perfusion CT image and time density curve corresponding to the regions of interest: center of the aorta as an arterial input reference (A), around entire tumor (T), around an area of muscle tissue immediately adjacent to the tumor (M), and random location in the normal muscle tissue distant from the tumor (N).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4401739&req=5

pone.0123196.g002: Axial perfusion CT image and time density curve corresponding to the regions of interest: center of the aorta as an arterial input reference (A), around entire tumor (T), around an area of muscle tissue immediately adjacent to the tumor (M), and random location in the normal muscle tissue distant from the tumor (N).
Mentions: The perfusion images were reconstructed from the raw projection data onto a 512 × 512-pixel matrix before being transferred to a syngo.via imaging workstation (Siemens Healthcare) for perfusion analysis. Absolute values of blood flow (BF), blood volume (BV), and capillary permeability-surface area (PMB) measurements were determined using CT Body Tumor Perfusion (Siemens Healthcare). At each of the four simultaneously imaged levels, three ROIs were drawn around the entire tumor (T), around an area of normal tissue immediately adjacent to the tumor (M), and in a random location within the normal muscle tissue (N) distant from the tumor as shown in Fig 2. Careful placement of arterial input function region (A) is the basis for accurate determination of CT Perfusion Map Construction, as shown in Fig 2. The BF, BV, and PMB measurements were calculated in each of the three ROIs (T, M, N). All ROIs and perfusion measurements were drawn and obtained by the same investigator. The characteristics of enhancement deduced from the perfusion CT were analyzed offline. All the differences before and after ablation were compared to confirm any recorded changes.

Bottom Line: The injection of the NaK-oil mixture resulted in significantly higher heat in the ablation areas.Perfusion computed tomography and its parameter map confirmed that the NaK-oil mixture had curative effects on VX2 tumors.With reference to the controlled thermal and chemical lethal injury to tumors, using a liquid alkali in ablation is potentially an effective and safe method to treat malignant tumors.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China; Department of Radiology, Haikou People's Hospital, Xiangya Medical School Central South University, Haikou City, Hainan, China.

ABSTRACT

Objective: Alkali metal appears to be a promising tool in thermochemical ablation, but, it requires additional data on safety is required. The objective of this study was to explore the effectiveness of permeable oil-packed liquid alkali metal in the thermochemical ablation of tumors.

Methods: Permeable oil-packed sodium-potassium (NaK) was prepared using ultrasonic mixing of different ratios of metal to oil. The thermal effect of the mixture during ablation of muscle tissue ex vivo was evaluated using the Fluke Ti400 Thermal Imager. The thermochemical effect of the NaK-oil mixture on VX2 tumors was evaluated by performing perfusion CT scans both before and after treatment in 10 VX2 rabbit model tumors. VX2 tumors were harvested from two rabbits immediately after treatment to assess their viability using trypan blue and hematoxylin and eosin (H.E.) staining.

Results: The injection of the NaK-oil mixture resulted in significantly higher heat in the ablation areas. The permeable oil controlled the rate of heat released during the NaK reaction with water in the living tissue. Perfusion computed tomography and its parameter map confirmed that the NaK-oil mixture had curative effects on VX2 tumors. Both trypan blue and H.E. staining showed partial necrosis of the VX2 tumors.

Conclusions: The NaK-oil mixture may be used successfully to ablate tumor tissue in vivo. With reference to the controlled thermal and chemical lethal injury to tumors, using a liquid alkali in ablation is potentially an effective and safe method to treat malignant tumors.

Show MeSH
Related in: MedlinePlus