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Thermochemical ablation therapy of VX2 tumor using a permeable oil-packed liquid alkali metal.

Guo Z, Zhang Q, Li X, Jing Z - PLoS ONE (2015)

Bottom Line: The injection of the NaK-oil mixture resulted in significantly higher heat in the ablation areas.Perfusion computed tomography and its parameter map confirmed that the NaK-oil mixture had curative effects on VX2 tumors.With reference to the controlled thermal and chemical lethal injury to tumors, using a liquid alkali in ablation is potentially an effective and safe method to treat malignant tumors.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China; Department of Radiology, Haikou People's Hospital, Xiangya Medical School Central South University, Haikou City, Hainan, China.

ABSTRACT

Objective: Alkali metal appears to be a promising tool in thermochemical ablation, but, it requires additional data on safety is required. The objective of this study was to explore the effectiveness of permeable oil-packed liquid alkali metal in the thermochemical ablation of tumors.

Methods: Permeable oil-packed sodium-potassium (NaK) was prepared using ultrasonic mixing of different ratios of metal to oil. The thermal effect of the mixture during ablation of muscle tissue ex vivo was evaluated using the Fluke Ti400 Thermal Imager. The thermochemical effect of the NaK-oil mixture on VX2 tumors was evaluated by performing perfusion CT scans both before and after treatment in 10 VX2 rabbit model tumors. VX2 tumors were harvested from two rabbits immediately after treatment to assess their viability using trypan blue and hematoxylin and eosin (H.E.) staining.

Results: The injection of the NaK-oil mixture resulted in significantly higher heat in the ablation areas. The permeable oil controlled the rate of heat released during the NaK reaction with water in the living tissue. Perfusion computed tomography and its parameter map confirmed that the NaK-oil mixture had curative effects on VX2 tumors. Both trypan blue and H.E. staining showed partial necrosis of the VX2 tumors.

Conclusions: The NaK-oil mixture may be used successfully to ablate tumor tissue in vivo. With reference to the controlled thermal and chemical lethal injury to tumors, using a liquid alkali in ablation is potentially an effective and safe method to treat malignant tumors.

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Related in: MedlinePlus

Flow chart for the preparation of the sodium-potassium (NaK) permeable-oil mixture.(A) first, accurately combine the permeable oil (FMS-121) and NaK in accordance with the volume ratio; second, inject NaK into the bottom of a 1.5-mL EP tube immersed in oil, and then carefully mix using ultrasonic probe vibration. (B) Prepare various ratios of the NaK–oil mixture encapsulated in syringes.
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pone.0123196.g001: Flow chart for the preparation of the sodium-potassium (NaK) permeable-oil mixture.(A) first, accurately combine the permeable oil (FMS-121) and NaK in accordance with the volume ratio; second, inject NaK into the bottom of a 1.5-mL EP tube immersed in oil, and then carefully mix using ultrasonic probe vibration. (B) Prepare various ratios of the NaK–oil mixture encapsulated in syringes.

Mentions: NaK is made by stirring Na and K at a 1:1 ratio under nitrogen at room temperature in a glass jar. Because NaK is a liquid at room temperature, it could be homogeneously mixed with polytrifluoropropylmethylsiloxane homopolymer (FMS-121, Alfa Aesar), which is water permeable [12], using the SKL150-IIN ultrasonic processor (Ningbo Sklon Lab Instrument Co., Ltd. Ningbo, China) at different ratios of oil and alloy (2:1, 5:1, 10:1). To mix the permeable oil and NaK homogeneously, NaK and the permeable oil were mixed using the following parameters: 5-seconds duration, 5-second intervals, 10 repetitions, and 150 W maximum output power. The stabilized uniform microdroplet containing NaK and permeable oil was disinfected using UV light at least 15 minutes before its use in the following tests (Fig 1).


Thermochemical ablation therapy of VX2 tumor using a permeable oil-packed liquid alkali metal.

Guo Z, Zhang Q, Li X, Jing Z - PLoS ONE (2015)

Flow chart for the preparation of the sodium-potassium (NaK) permeable-oil mixture.(A) first, accurately combine the permeable oil (FMS-121) and NaK in accordance with the volume ratio; second, inject NaK into the bottom of a 1.5-mL EP tube immersed in oil, and then carefully mix using ultrasonic probe vibration. (B) Prepare various ratios of the NaK–oil mixture encapsulated in syringes.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4401739&req=5

pone.0123196.g001: Flow chart for the preparation of the sodium-potassium (NaK) permeable-oil mixture.(A) first, accurately combine the permeable oil (FMS-121) and NaK in accordance with the volume ratio; second, inject NaK into the bottom of a 1.5-mL EP tube immersed in oil, and then carefully mix using ultrasonic probe vibration. (B) Prepare various ratios of the NaK–oil mixture encapsulated in syringes.
Mentions: NaK is made by stirring Na and K at a 1:1 ratio under nitrogen at room temperature in a glass jar. Because NaK is a liquid at room temperature, it could be homogeneously mixed with polytrifluoropropylmethylsiloxane homopolymer (FMS-121, Alfa Aesar), which is water permeable [12], using the SKL150-IIN ultrasonic processor (Ningbo Sklon Lab Instrument Co., Ltd. Ningbo, China) at different ratios of oil and alloy (2:1, 5:1, 10:1). To mix the permeable oil and NaK homogeneously, NaK and the permeable oil were mixed using the following parameters: 5-seconds duration, 5-second intervals, 10 repetitions, and 150 W maximum output power. The stabilized uniform microdroplet containing NaK and permeable oil was disinfected using UV light at least 15 minutes before its use in the following tests (Fig 1).

Bottom Line: The injection of the NaK-oil mixture resulted in significantly higher heat in the ablation areas.Perfusion computed tomography and its parameter map confirmed that the NaK-oil mixture had curative effects on VX2 tumors.With reference to the controlled thermal and chemical lethal injury to tumors, using a liquid alkali in ablation is potentially an effective and safe method to treat malignant tumors.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China; Department of Radiology, Haikou People's Hospital, Xiangya Medical School Central South University, Haikou City, Hainan, China.

ABSTRACT

Objective: Alkali metal appears to be a promising tool in thermochemical ablation, but, it requires additional data on safety is required. The objective of this study was to explore the effectiveness of permeable oil-packed liquid alkali metal in the thermochemical ablation of tumors.

Methods: Permeable oil-packed sodium-potassium (NaK) was prepared using ultrasonic mixing of different ratios of metal to oil. The thermal effect of the mixture during ablation of muscle tissue ex vivo was evaluated using the Fluke Ti400 Thermal Imager. The thermochemical effect of the NaK-oil mixture on VX2 tumors was evaluated by performing perfusion CT scans both before and after treatment in 10 VX2 rabbit model tumors. VX2 tumors were harvested from two rabbits immediately after treatment to assess their viability using trypan blue and hematoxylin and eosin (H.E.) staining.

Results: The injection of the NaK-oil mixture resulted in significantly higher heat in the ablation areas. The permeable oil controlled the rate of heat released during the NaK reaction with water in the living tissue. Perfusion computed tomography and its parameter map confirmed that the NaK-oil mixture had curative effects on VX2 tumors. Both trypan blue and H.E. staining showed partial necrosis of the VX2 tumors.

Conclusions: The NaK-oil mixture may be used successfully to ablate tumor tissue in vivo. With reference to the controlled thermal and chemical lethal injury to tumors, using a liquid alkali in ablation is potentially an effective and safe method to treat malignant tumors.

Show MeSH
Related in: MedlinePlus