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Banana Ovate family protein MaOFP1 and MADS-box protein MuMADS1 antagonistically regulated banana fruit ripening.

Liu J, Zhang J, Hu W, Miao H, Zhang J, Jia C, Wang Z, Xu B, Jin Z - PLoS ONE (2015)

Bottom Line: Meanwhile, MuMADS1 and MaOFP1 expression was highly stimulated and greatly suppressed, respectively, by exogenous ethylene.In contrast, MaOFP1 expression was highly stimulated while MuMADS1 was greatly suppressed by the ethylene competitor 1-methylcyclopropene (1-MCP).These results indicate that MuMADS1 and MaOFP1 are antagonistically regulated by ethylene and might play important roles in postharvest banana fruit ripening.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, China.

ABSTRACT
The ovate family protein named MaOFP1 was identified in banana (Musa acuminata L.AAA) fruit by a yeast two-hybrid (Y2H) method using the banana MADS-box gene MuMADS1 as bait and a 2 day postharvest (DPH) banana fruit cDNA library as prey. The interaction between MuMADS1 and MaOFP1 was further confirmed by Y2H and Bimolecular Fluorescence Complementation (BiFC) methods, which showed that the MuMADS1 K domain interacted with MaOFP1. Real-time quantitative PCR evaluation of MuMADS1 and MaOFP1 expression patterns in banana showed that they are highly expressed in 0 DPH fruit, but present in low levels in the stem, which suggests that simultaneous but different expression patterns exist for both MuMADS1 and MaOFP1 in different tissues and developing fruits. Meanwhile, MuMADS1 and MaOFP1 expression was highly stimulated and greatly suppressed, respectively, by exogenous ethylene. In contrast, MaOFP1 expression was highly stimulated while MuMADS1 was greatly suppressed by the ethylene competitor 1-methylcyclopropene (1-MCP). These results indicate that MuMADS1 and MaOFP1 are antagonistically regulated by ethylene and might play important roles in postharvest banana fruit ripening.

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Relative expression of MuMADS1 and MaOFP1 in naturally ripened (A, B), ethylene-treated (C, D), and 1-MCP-treated (E, F) bananas.The x-axis represents the days postharvest and the y-axis represents relative expression of MuMADS1 and MaOFP1 with respect to actin. Vertical bars on each column indicate SE from three replications. When absent, the bars fall within the dimensions of the symbol.
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pone.0123870.g005: Relative expression of MuMADS1 and MaOFP1 in naturally ripened (A, B), ethylene-treated (C, D), and 1-MCP-treated (E, F) bananas.The x-axis represents the days postharvest and the y-axis represents relative expression of MuMADS1 and MaOFP1 with respect to actin. Vertical bars on each column indicate SE from three replications. When absent, the bars fall within the dimensions of the symbol.

Mentions: The expression pattern of MuMADS1 in naturally ripened fruits gradually increased and peaked at 4.5 at 12 DPH, after which the level decreased. However, MaOFP1 expression gradually decreased with the fruit ripening process (Fig 5A and 5B). After treatment with exogenous ethylene, MuMADS1 expression quickly increased and reached a peak of 10.69 at 5 DPH, which is 7 days earlier than naturally ripened fruits. However, the expression of MaOFP1 was greatly suppressed and maintained at very low levels throughout the whole ripening process (Fig 5C and 5D). Meanwhile, expression of MuMADS1 was strongly suppressed and maintained at low levels following treatment with the ethylene competitor 1-MCP, but MaOFP1 expression was strongly increased and reached a peak of 2.1 at 8 DPH and then decreased (Fig 5E and 5F). This result indicated that MuMADS1 and MaOFP1 are antagonistically regulated by ethylene.


Banana Ovate family protein MaOFP1 and MADS-box protein MuMADS1 antagonistically regulated banana fruit ripening.

Liu J, Zhang J, Hu W, Miao H, Zhang J, Jia C, Wang Z, Xu B, Jin Z - PLoS ONE (2015)

Relative expression of MuMADS1 and MaOFP1 in naturally ripened (A, B), ethylene-treated (C, D), and 1-MCP-treated (E, F) bananas.The x-axis represents the days postharvest and the y-axis represents relative expression of MuMADS1 and MaOFP1 with respect to actin. Vertical bars on each column indicate SE from three replications. When absent, the bars fall within the dimensions of the symbol.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4401719&req=5

pone.0123870.g005: Relative expression of MuMADS1 and MaOFP1 in naturally ripened (A, B), ethylene-treated (C, D), and 1-MCP-treated (E, F) bananas.The x-axis represents the days postharvest and the y-axis represents relative expression of MuMADS1 and MaOFP1 with respect to actin. Vertical bars on each column indicate SE from three replications. When absent, the bars fall within the dimensions of the symbol.
Mentions: The expression pattern of MuMADS1 in naturally ripened fruits gradually increased and peaked at 4.5 at 12 DPH, after which the level decreased. However, MaOFP1 expression gradually decreased with the fruit ripening process (Fig 5A and 5B). After treatment with exogenous ethylene, MuMADS1 expression quickly increased and reached a peak of 10.69 at 5 DPH, which is 7 days earlier than naturally ripened fruits. However, the expression of MaOFP1 was greatly suppressed and maintained at very low levels throughout the whole ripening process (Fig 5C and 5D). Meanwhile, expression of MuMADS1 was strongly suppressed and maintained at low levels following treatment with the ethylene competitor 1-MCP, but MaOFP1 expression was strongly increased and reached a peak of 2.1 at 8 DPH and then decreased (Fig 5E and 5F). This result indicated that MuMADS1 and MaOFP1 are antagonistically regulated by ethylene.

Bottom Line: Meanwhile, MuMADS1 and MaOFP1 expression was highly stimulated and greatly suppressed, respectively, by exogenous ethylene.In contrast, MaOFP1 expression was highly stimulated while MuMADS1 was greatly suppressed by the ethylene competitor 1-methylcyclopropene (1-MCP).These results indicate that MuMADS1 and MaOFP1 are antagonistically regulated by ethylene and might play important roles in postharvest banana fruit ripening.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, China.

ABSTRACT
The ovate family protein named MaOFP1 was identified in banana (Musa acuminata L.AAA) fruit by a yeast two-hybrid (Y2H) method using the banana MADS-box gene MuMADS1 as bait and a 2 day postharvest (DPH) banana fruit cDNA library as prey. The interaction between MuMADS1 and MaOFP1 was further confirmed by Y2H and Bimolecular Fluorescence Complementation (BiFC) methods, which showed that the MuMADS1 K domain interacted with MaOFP1. Real-time quantitative PCR evaluation of MuMADS1 and MaOFP1 expression patterns in banana showed that they are highly expressed in 0 DPH fruit, but present in low levels in the stem, which suggests that simultaneous but different expression patterns exist for both MuMADS1 and MaOFP1 in different tissues and developing fruits. Meanwhile, MuMADS1 and MaOFP1 expression was highly stimulated and greatly suppressed, respectively, by exogenous ethylene. In contrast, MaOFP1 expression was highly stimulated while MuMADS1 was greatly suppressed by the ethylene competitor 1-methylcyclopropene (1-MCP). These results indicate that MuMADS1 and MaOFP1 are antagonistically regulated by ethylene and might play important roles in postharvest banana fruit ripening.

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