Limits...
Banana Ovate family protein MaOFP1 and MADS-box protein MuMADS1 antagonistically regulated banana fruit ripening.

Liu J, Zhang J, Hu W, Miao H, Zhang J, Jia C, Wang Z, Xu B, Jin Z - PLoS ONE (2015)

Bottom Line: Meanwhile, MuMADS1 and MaOFP1 expression was highly stimulated and greatly suppressed, respectively, by exogenous ethylene.In contrast, MaOFP1 expression was highly stimulated while MuMADS1 was greatly suppressed by the ethylene competitor 1-methylcyclopropene (1-MCP).These results indicate that MuMADS1 and MaOFP1 are antagonistically regulated by ethylene and might play important roles in postharvest banana fruit ripening.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, China.

ABSTRACT
The ovate family protein named MaOFP1 was identified in banana (Musa acuminata L.AAA) fruit by a yeast two-hybrid (Y2H) method using the banana MADS-box gene MuMADS1 as bait and a 2 day postharvest (DPH) banana fruit cDNA library as prey. The interaction between MuMADS1 and MaOFP1 was further confirmed by Y2H and Bimolecular Fluorescence Complementation (BiFC) methods, which showed that the MuMADS1 K domain interacted with MaOFP1. Real-time quantitative PCR evaluation of MuMADS1 and MaOFP1 expression patterns in banana showed that they are highly expressed in 0 DPH fruit, but present in low levels in the stem, which suggests that simultaneous but different expression patterns exist for both MuMADS1 and MaOFP1 in different tissues and developing fruits. Meanwhile, MuMADS1 and MaOFP1 expression was highly stimulated and greatly suppressed, respectively, by exogenous ethylene. In contrast, MaOFP1 expression was highly stimulated while MuMADS1 was greatly suppressed by the ethylene competitor 1-methylcyclopropene (1-MCP). These results indicate that MuMADS1 and MaOFP1 are antagonistically regulated by ethylene and might play important roles in postharvest banana fruit ripening.

Show MeSH
Relative expression of MuMADS1 and MaOFP1 in different tissues and developing fruits.(A) Different tissues and developing fruits: R: root; S; stem; L: leaf; Fl: flower; Ov8: Ovary 8 stage; Ov4: Ovary 4 stage; Ov1: Ovary 1 stage; 0DFr: 0 DPH fruits. (B)The x-axis represents the different tissues and developing fruits. The y-axis represents relative expression of MuMADS1 and MaOFP1 with respect to actin. Vertical bars on each column indicate SE from three replications.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4401719&req=5

pone.0123870.g004: Relative expression of MuMADS1 and MaOFP1 in different tissues and developing fruits.(A) Different tissues and developing fruits: R: root; S; stem; L: leaf; Fl: flower; Ov8: Ovary 8 stage; Ov4: Ovary 4 stage; Ov1: Ovary 1 stage; 0DFr: 0 DPH fruits. (B)The x-axis represents the different tissues and developing fruits. The y-axis represents relative expression of MuMADS1 and MaOFP1 with respect to actin. Vertical bars on each column indicate SE from three replications.

Mentions: RT-PCR analysis showed that both MuMADS1 and MaOFP1 were mainly expressed in the ovary 4 (Ov4), Ov1 stages and postharvest 0 DPH fruits. In 0 DPH fruits, MuMADS1 and MaOFP1 reached relative expression levels of 31.13 and 59.72, respectively. Both genes were poorly expressed in the stems with relative expression levels of 0.62 and 0.36, respectively (Fig 4). Together, these results suggest that MuMADS1 and MaOFP1 were simultaneous but different expression in different tissues and fruit developmental stages.


Banana Ovate family protein MaOFP1 and MADS-box protein MuMADS1 antagonistically regulated banana fruit ripening.

Liu J, Zhang J, Hu W, Miao H, Zhang J, Jia C, Wang Z, Xu B, Jin Z - PLoS ONE (2015)

Relative expression of MuMADS1 and MaOFP1 in different tissues and developing fruits.(A) Different tissues and developing fruits: R: root; S; stem; L: leaf; Fl: flower; Ov8: Ovary 8 stage; Ov4: Ovary 4 stage; Ov1: Ovary 1 stage; 0DFr: 0 DPH fruits. (B)The x-axis represents the different tissues and developing fruits. The y-axis represents relative expression of MuMADS1 and MaOFP1 with respect to actin. Vertical bars on each column indicate SE from three replications.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4401719&req=5

pone.0123870.g004: Relative expression of MuMADS1 and MaOFP1 in different tissues and developing fruits.(A) Different tissues and developing fruits: R: root; S; stem; L: leaf; Fl: flower; Ov8: Ovary 8 stage; Ov4: Ovary 4 stage; Ov1: Ovary 1 stage; 0DFr: 0 DPH fruits. (B)The x-axis represents the different tissues and developing fruits. The y-axis represents relative expression of MuMADS1 and MaOFP1 with respect to actin. Vertical bars on each column indicate SE from three replications.
Mentions: RT-PCR analysis showed that both MuMADS1 and MaOFP1 were mainly expressed in the ovary 4 (Ov4), Ov1 stages and postharvest 0 DPH fruits. In 0 DPH fruits, MuMADS1 and MaOFP1 reached relative expression levels of 31.13 and 59.72, respectively. Both genes were poorly expressed in the stems with relative expression levels of 0.62 and 0.36, respectively (Fig 4). Together, these results suggest that MuMADS1 and MaOFP1 were simultaneous but different expression in different tissues and fruit developmental stages.

Bottom Line: Meanwhile, MuMADS1 and MaOFP1 expression was highly stimulated and greatly suppressed, respectively, by exogenous ethylene.In contrast, MaOFP1 expression was highly stimulated while MuMADS1 was greatly suppressed by the ethylene competitor 1-methylcyclopropene (1-MCP).These results indicate that MuMADS1 and MaOFP1 are antagonistically regulated by ethylene and might play important roles in postharvest banana fruit ripening.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, China.

ABSTRACT
The ovate family protein named MaOFP1 was identified in banana (Musa acuminata L.AAA) fruit by a yeast two-hybrid (Y2H) method using the banana MADS-box gene MuMADS1 as bait and a 2 day postharvest (DPH) banana fruit cDNA library as prey. The interaction between MuMADS1 and MaOFP1 was further confirmed by Y2H and Bimolecular Fluorescence Complementation (BiFC) methods, which showed that the MuMADS1 K domain interacted with MaOFP1. Real-time quantitative PCR evaluation of MuMADS1 and MaOFP1 expression patterns in banana showed that they are highly expressed in 0 DPH fruit, but present in low levels in the stem, which suggests that simultaneous but different expression patterns exist for both MuMADS1 and MaOFP1 in different tissues and developing fruits. Meanwhile, MuMADS1 and MaOFP1 expression was highly stimulated and greatly suppressed, respectively, by exogenous ethylene. In contrast, MaOFP1 expression was highly stimulated while MuMADS1 was greatly suppressed by the ethylene competitor 1-methylcyclopropene (1-MCP). These results indicate that MuMADS1 and MaOFP1 are antagonistically regulated by ethylene and might play important roles in postharvest banana fruit ripening.

Show MeSH