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Identification of glutathione S-transferase (GST) genes from a dark septate endophytic fungus (Exophiala pisciphila) and their expression patterns under varied metals stress.

Shen M, Zhao DK, Qiao Q, Liu L, Wang JL, Cao GH, Li T, Zhao ZW - PLoS ONE (2015)

Bottom Line: Moreover, the variable expression patterns of these EpGSTs were observed under different heavy metal stresses at their effective concentrations for inhibiting growth by 50% (EC50).These results indicate that E.Pisciphila harbored a diverse of GST genes and the up-regulated EpGSTs are closely related to the heavy metal tolerance of E. pisciphila.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Conservation and Utilization for Bioresources and Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan University, Kunming, Yunnan, China.

ABSTRACT
Glutathione S-transferases (GSTs) compose a family of multifunctional enzymes that play important roles in the detoxification of xenobiotics and the oxidative stress response. In the present study, twenty four GST genes from the transcriptome of a metal-tolerant dark septate endophyte (DSE), Exophiala pisciphila, were identified based on sequence homology, and their responses to various heavy metal exposures were also analyzed. Phylogenetic analysis showed that the 24 GST genes from E. pisciphila (EpGSTs) were divided into eight distinct classes, including seven cytosolic classes and one mitochondrial metaxin 1-like class. Moreover, the variable expression patterns of these EpGSTs were observed under different heavy metal stresses at their effective concentrations for inhibiting growth by 50% (EC50). Lead (Pb) exposure caused the up-regulation of all EpGSTs, while cadmium (Cd), copper (Cu) and zinc (Zn) treatments led to the significant up-regulation of most of the EpGSTs (p < 0.05 to p < 0.001). Furthermore, although heavy metal-specific differences in performance were observed under various heavy metals in Escherichia coli BL21 (DE3) transformed with EpGSTN-31, the over-expression of this gene was able to enhance the heavy metal tolerance of the host cells. These results indicate that E. Pisciphila harbored a diverse of GST genes and the up-regulated EpGSTs are closely related to the heavy metal tolerance of E. pisciphila. The study represents the first investigation of the GST family in E. pisciphila and provides a primary interpretation of heavy metal detoxification for E. pisciphila.

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qRT-PCR of EpGSTs expression under stress of various heavy metals (Cd, Pb, Cu, and Zn).EpGSTs mRNA expression levels were calculated relative to β-tublin expression and are shown as the mean ± SE (n = 3). Asterisks indicate significant differences (p < 0.05*, p < 0.01**, p < 0.001***) compared to the control according to One-Sample T test.
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pone.0123418.g002: qRT-PCR of EpGSTs expression under stress of various heavy metals (Cd, Pb, Cu, and Zn).EpGSTs mRNA expression levels were calculated relative to β-tublin expression and are shown as the mean ± SE (n = 3). Asterisks indicate significant differences (p < 0.05*, p < 0.01**, p < 0.001***) compared to the control according to One-Sample T test.

Mentions: According to the qRT-PCR results, the GST mRNAs in E. pisciphila showed differential expression patterns during Cd exposure at its EC50 concentration (Fig 2A; S4 Table). The expression levels of all genes in the classes of EpGSTN-3, EpGSTN-2, EpGSTG, EpMetaxin1, and EpGSTZ were significantly up-regulated (p < 0.05 to p < 0.01, Fig 2A; S4 Table), except for those of EpGSTN-33 and EpGSTG2 genes which showed no significant change. And the highest expression was observed in EpGSTN-31 with the relative expression of 4.82-fold of its control (p < 0.01, Fig 2A; S4 Table). However, as concerned to the class of EpUre2p, the expression varied greatly among the genes. EpUre2p3, EpUre2p8, and EpUre2p9 transcripts were significantly up-regulated (p < 0.05 to p < 0.01), while the expression levels of EpUre2p2, and EpUre2p7 were significantly down-regulated (p < 0.05 to p < 0.01), and no significant change was observed in the expression of other genes in this class (Fig 2A; S4 Table).


Identification of glutathione S-transferase (GST) genes from a dark septate endophytic fungus (Exophiala pisciphila) and their expression patterns under varied metals stress.

Shen M, Zhao DK, Qiao Q, Liu L, Wang JL, Cao GH, Li T, Zhao ZW - PLoS ONE (2015)

qRT-PCR of EpGSTs expression under stress of various heavy metals (Cd, Pb, Cu, and Zn).EpGSTs mRNA expression levels were calculated relative to β-tublin expression and are shown as the mean ± SE (n = 3). Asterisks indicate significant differences (p < 0.05*, p < 0.01**, p < 0.001***) compared to the control according to One-Sample T test.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4401685&req=5

pone.0123418.g002: qRT-PCR of EpGSTs expression under stress of various heavy metals (Cd, Pb, Cu, and Zn).EpGSTs mRNA expression levels were calculated relative to β-tublin expression and are shown as the mean ± SE (n = 3). Asterisks indicate significant differences (p < 0.05*, p < 0.01**, p < 0.001***) compared to the control according to One-Sample T test.
Mentions: According to the qRT-PCR results, the GST mRNAs in E. pisciphila showed differential expression patterns during Cd exposure at its EC50 concentration (Fig 2A; S4 Table). The expression levels of all genes in the classes of EpGSTN-3, EpGSTN-2, EpGSTG, EpMetaxin1, and EpGSTZ were significantly up-regulated (p < 0.05 to p < 0.01, Fig 2A; S4 Table), except for those of EpGSTN-33 and EpGSTG2 genes which showed no significant change. And the highest expression was observed in EpGSTN-31 with the relative expression of 4.82-fold of its control (p < 0.01, Fig 2A; S4 Table). However, as concerned to the class of EpUre2p, the expression varied greatly among the genes. EpUre2p3, EpUre2p8, and EpUre2p9 transcripts were significantly up-regulated (p < 0.05 to p < 0.01), while the expression levels of EpUre2p2, and EpUre2p7 were significantly down-regulated (p < 0.05 to p < 0.01), and no significant change was observed in the expression of other genes in this class (Fig 2A; S4 Table).

Bottom Line: Moreover, the variable expression patterns of these EpGSTs were observed under different heavy metal stresses at their effective concentrations for inhibiting growth by 50% (EC50).These results indicate that E.Pisciphila harbored a diverse of GST genes and the up-regulated EpGSTs are closely related to the heavy metal tolerance of E. pisciphila.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Conservation and Utilization for Bioresources and Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan University, Kunming, Yunnan, China.

ABSTRACT
Glutathione S-transferases (GSTs) compose a family of multifunctional enzymes that play important roles in the detoxification of xenobiotics and the oxidative stress response. In the present study, twenty four GST genes from the transcriptome of a metal-tolerant dark septate endophyte (DSE), Exophiala pisciphila, were identified based on sequence homology, and their responses to various heavy metal exposures were also analyzed. Phylogenetic analysis showed that the 24 GST genes from E. pisciphila (EpGSTs) were divided into eight distinct classes, including seven cytosolic classes and one mitochondrial metaxin 1-like class. Moreover, the variable expression patterns of these EpGSTs were observed under different heavy metal stresses at their effective concentrations for inhibiting growth by 50% (EC50). Lead (Pb) exposure caused the up-regulation of all EpGSTs, while cadmium (Cd), copper (Cu) and zinc (Zn) treatments led to the significant up-regulation of most of the EpGSTs (p < 0.05 to p < 0.001). Furthermore, although heavy metal-specific differences in performance were observed under various heavy metals in Escherichia coli BL21 (DE3) transformed with EpGSTN-31, the over-expression of this gene was able to enhance the heavy metal tolerance of the host cells. These results indicate that E. Pisciphila harbored a diverse of GST genes and the up-regulated EpGSTs are closely related to the heavy metal tolerance of E. pisciphila. The study represents the first investigation of the GST family in E. pisciphila and provides a primary interpretation of heavy metal detoxification for E. pisciphila.

Show MeSH
Related in: MedlinePlus