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Use of poly (amidoamine) dendrimer for dentinal tubule occlusion: a preliminary study.

Wang T, Yang S, Wang L, Feng H - PLoS ONE (2015)

Bottom Line: The in situ mineralization within dentinal tubules is a promising treatment for dentin hypersensitivity as it induces the formation of mineral on the sensitive regions and occludes the dentinal tubules.These results showed that G3.0 PAMAM dendrimers coated on dentin surface and infiltrated in dentinal tubules could induce hydroxyapatite formation and resulted in effective dentinal tubule occlusion.This could lead to the development of a new therapeutic technique for the treatment of dentin hypersensitivity.

View Article: PubMed Central - PubMed

Affiliation: Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing, China.

ABSTRACT
The occlusion of dentinal tubules is an effective method to alleviate the symptoms caused by dentin hypersensitivity, a significant health problem in dentistry and daily life. The in situ mineralization within dentinal tubules is a promising treatment for dentin hypersensitivity as it induces the formation of mineral on the sensitive regions and occludes the dentinal tubules. This study was carried out to evaluate the in vitro effect of a whole generation poly(amidoamine) (PAMAM) dendrimer (G3.0) on dentinal tubule occlusion by inducing mineralization within dentinal tubules. Dentin discs were treated with PAMAM dendrimers using two methods, followed by the in vitro characterization using Attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), Field emission scanning electron microscopy (FE-SEM) and Energy-Dispersive X-ray Spectroscopy (EDS). These results showed that G3.0 PAMAM dendrimers coated on dentin surface and infiltrated in dentinal tubules could induce hydroxyapatite formation and resulted in effective dentinal tubule occlusion. Moreover, crosslinked PAMAM dendrimers could induce the remineralization of demineralized dentin and thus had the potential in dentinal tubule occlusion. In this in vitro study, dentinal tubules occlusion could be achieved by using PAMAM dendrimers. This could lead to the development of a new therapeutic technique for the treatment of dentin hypersensitivity.

No MeSH data available.


Related in: MedlinePlus

XRD spectra of EDTA demineralized dentin discs crosslinked with PAMAM dendrimers.(a) Neutral EDTA demineralized dentin (black line). (b) PAMAM dendrimers crosslinked demineralized dentin mineralized in SBF for 1w (red line). (c) Sound dentin (blue line).
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pone.0124735.g005: XRD spectra of EDTA demineralized dentin discs crosslinked with PAMAM dendrimers.(a) Neutral EDTA demineralized dentin (black line). (b) PAMAM dendrimers crosslinked demineralized dentin mineralized in SBF for 1w (red line). (c) Sound dentin (blue line).

Mentions: Fig 5 showed the XRD results of the surface of the demineralized dentin discs, before and after they were crosslinked with PAMAM dendrimers. (XRD measurements of PAMAM coated dentin discs before and after they were immersed in SBF for 1w were not carried out because the surface of dentin discs which was not covered by mineral crystals was suggested to interfere with the results.) Fig 5a shows the XRD pattern of the demineralized dentin discs. The broader and shorter peaks between 30° and 35° indicate that the inorganic phase on the surface of the demineralized dentin discs lacked a crystal lattice. The broader peak at 20° indicates the presence of collagen. The XRD pattern of the biomimetically remineralized dentin discs (Fig 5c) shows the characteristic diffraction peaks of HAP, with a peak corresponding to the (0 0 2) plane and overlapping peaks corresponding to the (2 1 1), (1 1 2) and (3 0 0) planes[26–28]. This result is very similar to the XRD pattern of sound dentin that is shown in Fig 5b.


Use of poly (amidoamine) dendrimer for dentinal tubule occlusion: a preliminary study.

Wang T, Yang S, Wang L, Feng H - PLoS ONE (2015)

XRD spectra of EDTA demineralized dentin discs crosslinked with PAMAM dendrimers.(a) Neutral EDTA demineralized dentin (black line). (b) PAMAM dendrimers crosslinked demineralized dentin mineralized in SBF for 1w (red line). (c) Sound dentin (blue line).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4401684&req=5

pone.0124735.g005: XRD spectra of EDTA demineralized dentin discs crosslinked with PAMAM dendrimers.(a) Neutral EDTA demineralized dentin (black line). (b) PAMAM dendrimers crosslinked demineralized dentin mineralized in SBF for 1w (red line). (c) Sound dentin (blue line).
Mentions: Fig 5 showed the XRD results of the surface of the demineralized dentin discs, before and after they were crosslinked with PAMAM dendrimers. (XRD measurements of PAMAM coated dentin discs before and after they were immersed in SBF for 1w were not carried out because the surface of dentin discs which was not covered by mineral crystals was suggested to interfere with the results.) Fig 5a shows the XRD pattern of the demineralized dentin discs. The broader and shorter peaks between 30° and 35° indicate that the inorganic phase on the surface of the demineralized dentin discs lacked a crystal lattice. The broader peak at 20° indicates the presence of collagen. The XRD pattern of the biomimetically remineralized dentin discs (Fig 5c) shows the characteristic diffraction peaks of HAP, with a peak corresponding to the (0 0 2) plane and overlapping peaks corresponding to the (2 1 1), (1 1 2) and (3 0 0) planes[26–28]. This result is very similar to the XRD pattern of sound dentin that is shown in Fig 5b.

Bottom Line: The in situ mineralization within dentinal tubules is a promising treatment for dentin hypersensitivity as it induces the formation of mineral on the sensitive regions and occludes the dentinal tubules.These results showed that G3.0 PAMAM dendrimers coated on dentin surface and infiltrated in dentinal tubules could induce hydroxyapatite formation and resulted in effective dentinal tubule occlusion.This could lead to the development of a new therapeutic technique for the treatment of dentin hypersensitivity.

View Article: PubMed Central - PubMed

Affiliation: Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing, China.

ABSTRACT
The occlusion of dentinal tubules is an effective method to alleviate the symptoms caused by dentin hypersensitivity, a significant health problem in dentistry and daily life. The in situ mineralization within dentinal tubules is a promising treatment for dentin hypersensitivity as it induces the formation of mineral on the sensitive regions and occludes the dentinal tubules. This study was carried out to evaluate the in vitro effect of a whole generation poly(amidoamine) (PAMAM) dendrimer (G3.0) on dentinal tubule occlusion by inducing mineralization within dentinal tubules. Dentin discs were treated with PAMAM dendrimers using two methods, followed by the in vitro characterization using Attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), Field emission scanning electron microscopy (FE-SEM) and Energy-Dispersive X-ray Spectroscopy (EDS). These results showed that G3.0 PAMAM dendrimers coated on dentin surface and infiltrated in dentinal tubules could induce hydroxyapatite formation and resulted in effective dentinal tubule occlusion. Moreover, crosslinked PAMAM dendrimers could induce the remineralization of demineralized dentin and thus had the potential in dentinal tubule occlusion. In this in vitro study, dentinal tubules occlusion could be achieved by using PAMAM dendrimers. This could lead to the development of a new therapeutic technique for the treatment of dentin hypersensitivity.

No MeSH data available.


Related in: MedlinePlus