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Use of poly (amidoamine) dendrimer for dentinal tubule occlusion: a preliminary study.

Wang T, Yang S, Wang L, Feng H - PLoS ONE (2015)

Bottom Line: The in situ mineralization within dentinal tubules is a promising treatment for dentin hypersensitivity as it induces the formation of mineral on the sensitive regions and occludes the dentinal tubules.These results showed that G3.0 PAMAM dendrimers coated on dentin surface and infiltrated in dentinal tubules could induce hydroxyapatite formation and resulted in effective dentinal tubule occlusion.This could lead to the development of a new therapeutic technique for the treatment of dentin hypersensitivity.

View Article: PubMed Central - PubMed

Affiliation: Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing, China.

ABSTRACT
The occlusion of dentinal tubules is an effective method to alleviate the symptoms caused by dentin hypersensitivity, a significant health problem in dentistry and daily life. The in situ mineralization within dentinal tubules is a promising treatment for dentin hypersensitivity as it induces the formation of mineral on the sensitive regions and occludes the dentinal tubules. This study was carried out to evaluate the in vitro effect of a whole generation poly(amidoamine) (PAMAM) dendrimer (G3.0) on dentinal tubule occlusion by inducing mineralization within dentinal tubules. Dentin discs were treated with PAMAM dendrimers using two methods, followed by the in vitro characterization using Attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), Field emission scanning electron microscopy (FE-SEM) and Energy-Dispersive X-ray Spectroscopy (EDS). These results showed that G3.0 PAMAM dendrimers coated on dentin surface and infiltrated in dentinal tubules could induce hydroxyapatite formation and resulted in effective dentinal tubule occlusion. Moreover, crosslinked PAMAM dendrimers could induce the remineralization of demineralized dentin and thus had the potential in dentinal tubule occlusion. In this in vitro study, dentinal tubules occlusion could be achieved by using PAMAM dendrimers. This could lead to the development of a new therapeutic technique for the treatment of dentin hypersensitivity.

No MeSH data available.


Related in: MedlinePlus

SEM images of the remineralization of demineralized dentin discs.(A, B) Dentin discs demineralized with neutral EDTA solution for 72h. (C, D) EDTA demineralized dentin discs crosslinked with G3.0 PAMAM dendrimers using 0.25% glutaraldehyde for 24h, exhibiting a “corn-on-the-cob” appearance. (E, F) Flake-like crystals were induced on the surface and covered the wall of dentinal tubules after PAMAM crosslinked dentin discs were treated with SBF for 1w, with dentinal tubules still left patent. (Insert of (E)) EDS showed the Ca/P molar ratio of the mineral crystals was 1.59±0.05. (G, H) No significant mineral crystals formed on the demineralized dentin discs after they were immersed in SBF for 1w, without treated with PAMAM dendrimers.
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pone.0124735.g004: SEM images of the remineralization of demineralized dentin discs.(A, B) Dentin discs demineralized with neutral EDTA solution for 72h. (C, D) EDTA demineralized dentin discs crosslinked with G3.0 PAMAM dendrimers using 0.25% glutaraldehyde for 24h, exhibiting a “corn-on-the-cob” appearance. (E, F) Flake-like crystals were induced on the surface and covered the wall of dentinal tubules after PAMAM crosslinked dentin discs were treated with SBF for 1w, with dentinal tubules still left patent. (Insert of (E)) EDS showed the Ca/P molar ratio of the mineral crystals was 1.59±0.05. (G, H) No significant mineral crystals formed on the demineralized dentin discs after they were immersed in SBF for 1w, without treated with PAMAM dendrimers.

Mentions: The surface morphologies of the demineralized dentin discs before and after crosslinked with PAMAM dendrimers were shown in Fig 4. As shown in Fig 4A and 4B, after the dentin discs were demineralized in neutral EDTA solution for 72 h, the smear layer was removed, the dentinal tubules were opened and the collagen fibrils were completely exposed. Collagen fibrils were thickened and embedded with spherical particles after demineralized dentin discs were treated with G3.0 PAMAM dendrimer using glutaraldehyde as crosslinking agent (Fig 4C and 4D). Flake-like crystals were induced on the surface of dentin discs and within dentinal tubules after PAMAM crosslinked dentin discs were treated with SBF for 1 week (Fig 4E and 4F). However, dentinal tubules were still patent although the walls of dentinal tubules were remineralized. X-ray spectroscopy (EDS) showed that the Ca/P molar ratio of the mineral crystals was 1.59±0.05 (Fig 4E insert), indicating that these mineral crystals were calcium-deficient HAP, as the Ca/P ratio of HAp is 1.67. Fig 4G and 4H showed that no significant mineral crystals formed on the demineralized dentin discs after they were immersed in SBF at 37°C for 1 week, without treated with PAMAM dendrimer.


Use of poly (amidoamine) dendrimer for dentinal tubule occlusion: a preliminary study.

Wang T, Yang S, Wang L, Feng H - PLoS ONE (2015)

SEM images of the remineralization of demineralized dentin discs.(A, B) Dentin discs demineralized with neutral EDTA solution for 72h. (C, D) EDTA demineralized dentin discs crosslinked with G3.0 PAMAM dendrimers using 0.25% glutaraldehyde for 24h, exhibiting a “corn-on-the-cob” appearance. (E, F) Flake-like crystals were induced on the surface and covered the wall of dentinal tubules after PAMAM crosslinked dentin discs were treated with SBF for 1w, with dentinal tubules still left patent. (Insert of (E)) EDS showed the Ca/P molar ratio of the mineral crystals was 1.59±0.05. (G, H) No significant mineral crystals formed on the demineralized dentin discs after they were immersed in SBF for 1w, without treated with PAMAM dendrimers.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4401684&req=5

pone.0124735.g004: SEM images of the remineralization of demineralized dentin discs.(A, B) Dentin discs demineralized with neutral EDTA solution for 72h. (C, D) EDTA demineralized dentin discs crosslinked with G3.0 PAMAM dendrimers using 0.25% glutaraldehyde for 24h, exhibiting a “corn-on-the-cob” appearance. (E, F) Flake-like crystals were induced on the surface and covered the wall of dentinal tubules after PAMAM crosslinked dentin discs were treated with SBF for 1w, with dentinal tubules still left patent. (Insert of (E)) EDS showed the Ca/P molar ratio of the mineral crystals was 1.59±0.05. (G, H) No significant mineral crystals formed on the demineralized dentin discs after they were immersed in SBF for 1w, without treated with PAMAM dendrimers.
Mentions: The surface morphologies of the demineralized dentin discs before and after crosslinked with PAMAM dendrimers were shown in Fig 4. As shown in Fig 4A and 4B, after the dentin discs were demineralized in neutral EDTA solution for 72 h, the smear layer was removed, the dentinal tubules were opened and the collagen fibrils were completely exposed. Collagen fibrils were thickened and embedded with spherical particles after demineralized dentin discs were treated with G3.0 PAMAM dendrimer using glutaraldehyde as crosslinking agent (Fig 4C and 4D). Flake-like crystals were induced on the surface of dentin discs and within dentinal tubules after PAMAM crosslinked dentin discs were treated with SBF for 1 week (Fig 4E and 4F). However, dentinal tubules were still patent although the walls of dentinal tubules were remineralized. X-ray spectroscopy (EDS) showed that the Ca/P molar ratio of the mineral crystals was 1.59±0.05 (Fig 4E insert), indicating that these mineral crystals were calcium-deficient HAP, as the Ca/P ratio of HAp is 1.67. Fig 4G and 4H showed that no significant mineral crystals formed on the demineralized dentin discs after they were immersed in SBF at 37°C for 1 week, without treated with PAMAM dendrimer.

Bottom Line: The in situ mineralization within dentinal tubules is a promising treatment for dentin hypersensitivity as it induces the formation of mineral on the sensitive regions and occludes the dentinal tubules.These results showed that G3.0 PAMAM dendrimers coated on dentin surface and infiltrated in dentinal tubules could induce hydroxyapatite formation and resulted in effective dentinal tubule occlusion.This could lead to the development of a new therapeutic technique for the treatment of dentin hypersensitivity.

View Article: PubMed Central - PubMed

Affiliation: Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing, China.

ABSTRACT
The occlusion of dentinal tubules is an effective method to alleviate the symptoms caused by dentin hypersensitivity, a significant health problem in dentistry and daily life. The in situ mineralization within dentinal tubules is a promising treatment for dentin hypersensitivity as it induces the formation of mineral on the sensitive regions and occludes the dentinal tubules. This study was carried out to evaluate the in vitro effect of a whole generation poly(amidoamine) (PAMAM) dendrimer (G3.0) on dentinal tubule occlusion by inducing mineralization within dentinal tubules. Dentin discs were treated with PAMAM dendrimers using two methods, followed by the in vitro characterization using Attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), Field emission scanning electron microscopy (FE-SEM) and Energy-Dispersive X-ray Spectroscopy (EDS). These results showed that G3.0 PAMAM dendrimers coated on dentin surface and infiltrated in dentinal tubules could induce hydroxyapatite formation and resulted in effective dentinal tubule occlusion. Moreover, crosslinked PAMAM dendrimers could induce the remineralization of demineralized dentin and thus had the potential in dentinal tubule occlusion. In this in vitro study, dentinal tubules occlusion could be achieved by using PAMAM dendrimers. This could lead to the development of a new therapeutic technique for the treatment of dentin hypersensitivity.

No MeSH data available.


Related in: MedlinePlus