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Use of poly (amidoamine) dendrimer for dentinal tubule occlusion: a preliminary study.

Wang T, Yang S, Wang L, Feng H - PLoS ONE (2015)

Bottom Line: The in situ mineralization within dentinal tubules is a promising treatment for dentin hypersensitivity as it induces the formation of mineral on the sensitive regions and occludes the dentinal tubules.These results showed that G3.0 PAMAM dendrimers coated on dentin surface and infiltrated in dentinal tubules could induce hydroxyapatite formation and resulted in effective dentinal tubule occlusion.This could lead to the development of a new therapeutic technique for the treatment of dentin hypersensitivity.

View Article: PubMed Central - PubMed

Affiliation: Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing, China.

ABSTRACT
The occlusion of dentinal tubules is an effective method to alleviate the symptoms caused by dentin hypersensitivity, a significant health problem in dentistry and daily life. The in situ mineralization within dentinal tubules is a promising treatment for dentin hypersensitivity as it induces the formation of mineral on the sensitive regions and occludes the dentinal tubules. This study was carried out to evaluate the in vitro effect of a whole generation poly(amidoamine) (PAMAM) dendrimer (G3.0) on dentinal tubule occlusion by inducing mineralization within dentinal tubules. Dentin discs were treated with PAMAM dendrimers using two methods, followed by the in vitro characterization using Attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), Field emission scanning electron microscopy (FE-SEM) and Energy-Dispersive X-ray Spectroscopy (EDS). These results showed that G3.0 PAMAM dendrimers coated on dentin surface and infiltrated in dentinal tubules could induce hydroxyapatite formation and resulted in effective dentinal tubule occlusion. Moreover, crosslinked PAMAM dendrimers could induce the remineralization of demineralized dentin and thus had the potential in dentinal tubule occlusion. In this in vitro study, dentinal tubules occlusion could be achieved by using PAMAM dendrimers. This could lead to the development of a new therapeutic technique for the treatment of dentin hypersensitivity.

No MeSH data available.


Related in: MedlinePlus

SEM images of dentin surface and longitudinal section morphology indicating dentinal tubules occlusion.(A, B) Dentin discs etched with 10% citric acid for 30s. All of the dentinal tubules were open. (C, D) Dentinal tubules occluded with newly formed rod-like crystals after dentin discs were coated with PAMAM dendrimers and immersed in SBF for 1w. The outermost part of tubules was left patent. (E, F) Dentinal tubules occluded with newly formed precipitates projecting form dentin surface. Part of the dentin surface was covered with globular and rod-like crystals. (G) Dentinal tubules occluded from the dentin surface to a depth up to approximately 20μm. (H) In control group, no significant difference was observed before and after dentin discs were directly immersed in SBF for 1w, without treated with PAMAM dendrimer.
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pone.0124735.g003: SEM images of dentin surface and longitudinal section morphology indicating dentinal tubules occlusion.(A, B) Dentin discs etched with 10% citric acid for 30s. All of the dentinal tubules were open. (C, D) Dentinal tubules occluded with newly formed rod-like crystals after dentin discs were coated with PAMAM dendrimers and immersed in SBF for 1w. The outermost part of tubules was left patent. (E, F) Dentinal tubules occluded with newly formed precipitates projecting form dentin surface. Part of the dentin surface was covered with globular and rod-like crystals. (G) Dentinal tubules occluded from the dentin surface to a depth up to approximately 20μm. (H) In control group, no significant difference was observed before and after dentin discs were directly immersed in SBF for 1w, without treated with PAMAM dendrimer.

Mentions: The SEM image of dentin surface treated with 10% citric acid solution for 30 seconds showed that all dentinal tubules were open (Fig 3A and 3B), similar to hypersensitive dentin, indicating that the smear layer produced during cutting process was removed and the tubules were cleansed as a result of the citric acid treatment. The dentin tubules were completely free from debris. The peritubular dentin was compact and homogeneous. After dentin discs were coated with pure G3.0 PAMAM dendrimer and immersed in SBF at 37°C for 1 week, the open dentinal tubules were completely occluded with precipitates (Fig 3C–3F) and two kinds of typical appearances were observed. One was where the newly formed rod-like crystals sealed the dentinal tubules as plugs. However, the outermost part of tubules was left patent (Fig 3C and 3D). In the other case, dentinal tubules were occluded with newly formed precipitates projecting form dentin surface (Fig 3E and 3F). Globular and rod-like crystals were combined with each other and covered parts of the dentin surface. Fig 3G showed the longitudinal section parallel to the alignment direction of dentinal tubules after PAMAM coated dentin discs were immersed in SBF for 1 week. As shown in the figure, dentinal tubules were occluded from the dentin surface to a depth of approximately 5μm. In addition, precipitates were observed to have a better sealing effect in parts of dentinal tubules, reaching a depth of approximately 20μm. In the control group, no significant difference was observed before and after dentin discs were directly immersed in SBF for 1 week, without treated with PAMAM dendrimer (Fig 3H).


Use of poly (amidoamine) dendrimer for dentinal tubule occlusion: a preliminary study.

Wang T, Yang S, Wang L, Feng H - PLoS ONE (2015)

SEM images of dentin surface and longitudinal section morphology indicating dentinal tubules occlusion.(A, B) Dentin discs etched with 10% citric acid for 30s. All of the dentinal tubules were open. (C, D) Dentinal tubules occluded with newly formed rod-like crystals after dentin discs were coated with PAMAM dendrimers and immersed in SBF for 1w. The outermost part of tubules was left patent. (E, F) Dentinal tubules occluded with newly formed precipitates projecting form dentin surface. Part of the dentin surface was covered with globular and rod-like crystals. (G) Dentinal tubules occluded from the dentin surface to a depth up to approximately 20μm. (H) In control group, no significant difference was observed before and after dentin discs were directly immersed in SBF for 1w, without treated with PAMAM dendrimer.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4401684&req=5

pone.0124735.g003: SEM images of dentin surface and longitudinal section morphology indicating dentinal tubules occlusion.(A, B) Dentin discs etched with 10% citric acid for 30s. All of the dentinal tubules were open. (C, D) Dentinal tubules occluded with newly formed rod-like crystals after dentin discs were coated with PAMAM dendrimers and immersed in SBF for 1w. The outermost part of tubules was left patent. (E, F) Dentinal tubules occluded with newly formed precipitates projecting form dentin surface. Part of the dentin surface was covered with globular and rod-like crystals. (G) Dentinal tubules occluded from the dentin surface to a depth up to approximately 20μm. (H) In control group, no significant difference was observed before and after dentin discs were directly immersed in SBF for 1w, without treated with PAMAM dendrimer.
Mentions: The SEM image of dentin surface treated with 10% citric acid solution for 30 seconds showed that all dentinal tubules were open (Fig 3A and 3B), similar to hypersensitive dentin, indicating that the smear layer produced during cutting process was removed and the tubules were cleansed as a result of the citric acid treatment. The dentin tubules were completely free from debris. The peritubular dentin was compact and homogeneous. After dentin discs were coated with pure G3.0 PAMAM dendrimer and immersed in SBF at 37°C for 1 week, the open dentinal tubules were completely occluded with precipitates (Fig 3C–3F) and two kinds of typical appearances were observed. One was where the newly formed rod-like crystals sealed the dentinal tubules as plugs. However, the outermost part of tubules was left patent (Fig 3C and 3D). In the other case, dentinal tubules were occluded with newly formed precipitates projecting form dentin surface (Fig 3E and 3F). Globular and rod-like crystals were combined with each other and covered parts of the dentin surface. Fig 3G showed the longitudinal section parallel to the alignment direction of dentinal tubules after PAMAM coated dentin discs were immersed in SBF for 1 week. As shown in the figure, dentinal tubules were occluded from the dentin surface to a depth of approximately 5μm. In addition, precipitates were observed to have a better sealing effect in parts of dentinal tubules, reaching a depth of approximately 20μm. In the control group, no significant difference was observed before and after dentin discs were directly immersed in SBF for 1 week, without treated with PAMAM dendrimer (Fig 3H).

Bottom Line: The in situ mineralization within dentinal tubules is a promising treatment for dentin hypersensitivity as it induces the formation of mineral on the sensitive regions and occludes the dentinal tubules.These results showed that G3.0 PAMAM dendrimers coated on dentin surface and infiltrated in dentinal tubules could induce hydroxyapatite formation and resulted in effective dentinal tubule occlusion.This could lead to the development of a new therapeutic technique for the treatment of dentin hypersensitivity.

View Article: PubMed Central - PubMed

Affiliation: Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing, China.

ABSTRACT
The occlusion of dentinal tubules is an effective method to alleviate the symptoms caused by dentin hypersensitivity, a significant health problem in dentistry and daily life. The in situ mineralization within dentinal tubules is a promising treatment for dentin hypersensitivity as it induces the formation of mineral on the sensitive regions and occludes the dentinal tubules. This study was carried out to evaluate the in vitro effect of a whole generation poly(amidoamine) (PAMAM) dendrimer (G3.0) on dentinal tubule occlusion by inducing mineralization within dentinal tubules. Dentin discs were treated with PAMAM dendrimers using two methods, followed by the in vitro characterization using Attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), Field emission scanning electron microscopy (FE-SEM) and Energy-Dispersive X-ray Spectroscopy (EDS). These results showed that G3.0 PAMAM dendrimers coated on dentin surface and infiltrated in dentinal tubules could induce hydroxyapatite formation and resulted in effective dentinal tubule occlusion. Moreover, crosslinked PAMAM dendrimers could induce the remineralization of demineralized dentin and thus had the potential in dentinal tubule occlusion. In this in vitro study, dentinal tubules occlusion could be achieved by using PAMAM dendrimers. This could lead to the development of a new therapeutic technique for the treatment of dentin hypersensitivity.

No MeSH data available.


Related in: MedlinePlus