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Use of poly (amidoamine) dendrimer for dentinal tubule occlusion: a preliminary study.

Wang T, Yang S, Wang L, Feng H - PLoS ONE (2015)

Bottom Line: The in situ mineralization within dentinal tubules is a promising treatment for dentin hypersensitivity as it induces the formation of mineral on the sensitive regions and occludes the dentinal tubules.These results showed that G3.0 PAMAM dendrimers coated on dentin surface and infiltrated in dentinal tubules could induce hydroxyapatite formation and resulted in effective dentinal tubule occlusion.This could lead to the development of a new therapeutic technique for the treatment of dentin hypersensitivity.

View Article: PubMed Central - PubMed

Affiliation: Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing, China.

ABSTRACT
The occlusion of dentinal tubules is an effective method to alleviate the symptoms caused by dentin hypersensitivity, a significant health problem in dentistry and daily life. The in situ mineralization within dentinal tubules is a promising treatment for dentin hypersensitivity as it induces the formation of mineral on the sensitive regions and occludes the dentinal tubules. This study was carried out to evaluate the in vitro effect of a whole generation poly(amidoamine) (PAMAM) dendrimer (G3.0) on dentinal tubule occlusion by inducing mineralization within dentinal tubules. Dentin discs were treated with PAMAM dendrimers using two methods, followed by the in vitro characterization using Attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), Field emission scanning electron microscopy (FE-SEM) and Energy-Dispersive X-ray Spectroscopy (EDS). These results showed that G3.0 PAMAM dendrimers coated on dentin surface and infiltrated in dentinal tubules could induce hydroxyapatite formation and resulted in effective dentinal tubule occlusion. Moreover, crosslinked PAMAM dendrimers could induce the remineralization of demineralized dentin and thus had the potential in dentinal tubule occlusion. In this in vitro study, dentinal tubules occlusion could be achieved by using PAMAM dendrimers. This could lead to the development of a new therapeutic technique for the treatment of dentin hypersensitivity.

No MeSH data available.


Related in: MedlinePlus

Infrared spectra (2,000–500 cm-1) of EDTA demineralized dentin discs crosslinked with PAMAM dendrimers.(a) Sound dentin (black line). (b) Dentin disc demineralized with 0.5 M neutral EDTA solution at room temperature for 72h (red line). (c) Demineralized dentin disc crosslinked with G3.0 PAMAM dendrimers using 0.25% glutaraldehyde at 4°C for 24h (green line). (d) PAMAM dendrimers crosslinked demineralized dentin disc immersed in pH 5.8 PBS for 2h (blue line). (e) Demineralized dentin disc immersed in pH 5.8 PBS for 2h without PAMAM dendrimer crosslinking (pink line).
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pone.0124735.g002: Infrared spectra (2,000–500 cm-1) of EDTA demineralized dentin discs crosslinked with PAMAM dendrimers.(a) Sound dentin (black line). (b) Dentin disc demineralized with 0.5 M neutral EDTA solution at room temperature for 72h (red line). (c) Demineralized dentin disc crosslinked with G3.0 PAMAM dendrimers using 0.25% glutaraldehyde at 4°C for 24h (green line). (d) PAMAM dendrimers crosslinked demineralized dentin disc immersed in pH 5.8 PBS for 2h (blue line). (e) Demineralized dentin disc immersed in pH 5.8 PBS for 2h without PAMAM dendrimer crosslinking (pink line).

Mentions: Fig 2 showed the ATR-FTIR spectra of demineralized dentin discs before and after treated with G3.0 PAMAM dendrimer using glutaraldehyde as crosslinking agent. Changes after the treatment of PAMAM crosslinked dentin discs with PBS were also shown. Prior to demineralization, the resonances around 1000 cm-1 (1008 and 959 cm-1) were attributed to PO43-(a). After the dentin discs were treated with neutral EDTA solution for 72 h, the ATR-FTIR analysis showed that the resonances at 1629 and 1556 cm-1, assigned as amide I and amide II bands of type I collagen[25], were strengthened, while the resonances attributed to PO43- were markedly weakened (b), indicating that the dentin discs were completely demineralized. After demineralized dentin discs were crosslinked with PAMAM dendrimer, the resonances assigned as amide I and amide II bands became more intense(c), indicating that PAMAM dendrimer molecules were successfully crosslinked to demineralized type I collagen fibrils. Compared to untreated demineralized dentin discs which were immersed in PBS for 2h (e), the appearance of new peaks assigned as PO43- (1067 and 1034 cm-1) after PAMAM crosslinked demineralized dentin discs were treated with PBS (d) indicated that PAMAM dendrimer molecules crosslinked to collagen fibrils of demineralized dentin discs had the capacity to attract PO43- through electrostatic interaction.


Use of poly (amidoamine) dendrimer for dentinal tubule occlusion: a preliminary study.

Wang T, Yang S, Wang L, Feng H - PLoS ONE (2015)

Infrared spectra (2,000–500 cm-1) of EDTA demineralized dentin discs crosslinked with PAMAM dendrimers.(a) Sound dentin (black line). (b) Dentin disc demineralized with 0.5 M neutral EDTA solution at room temperature for 72h (red line). (c) Demineralized dentin disc crosslinked with G3.0 PAMAM dendrimers using 0.25% glutaraldehyde at 4°C for 24h (green line). (d) PAMAM dendrimers crosslinked demineralized dentin disc immersed in pH 5.8 PBS for 2h (blue line). (e) Demineralized dentin disc immersed in pH 5.8 PBS for 2h without PAMAM dendrimer crosslinking (pink line).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4401684&req=5

pone.0124735.g002: Infrared spectra (2,000–500 cm-1) of EDTA demineralized dentin discs crosslinked with PAMAM dendrimers.(a) Sound dentin (black line). (b) Dentin disc demineralized with 0.5 M neutral EDTA solution at room temperature for 72h (red line). (c) Demineralized dentin disc crosslinked with G3.0 PAMAM dendrimers using 0.25% glutaraldehyde at 4°C for 24h (green line). (d) PAMAM dendrimers crosslinked demineralized dentin disc immersed in pH 5.8 PBS for 2h (blue line). (e) Demineralized dentin disc immersed in pH 5.8 PBS for 2h without PAMAM dendrimer crosslinking (pink line).
Mentions: Fig 2 showed the ATR-FTIR spectra of demineralized dentin discs before and after treated with G3.0 PAMAM dendrimer using glutaraldehyde as crosslinking agent. Changes after the treatment of PAMAM crosslinked dentin discs with PBS were also shown. Prior to demineralization, the resonances around 1000 cm-1 (1008 and 959 cm-1) were attributed to PO43-(a). After the dentin discs were treated with neutral EDTA solution for 72 h, the ATR-FTIR analysis showed that the resonances at 1629 and 1556 cm-1, assigned as amide I and amide II bands of type I collagen[25], were strengthened, while the resonances attributed to PO43- were markedly weakened (b), indicating that the dentin discs were completely demineralized. After demineralized dentin discs were crosslinked with PAMAM dendrimer, the resonances assigned as amide I and amide II bands became more intense(c), indicating that PAMAM dendrimer molecules were successfully crosslinked to demineralized type I collagen fibrils. Compared to untreated demineralized dentin discs which were immersed in PBS for 2h (e), the appearance of new peaks assigned as PO43- (1067 and 1034 cm-1) after PAMAM crosslinked demineralized dentin discs were treated with PBS (d) indicated that PAMAM dendrimer molecules crosslinked to collagen fibrils of demineralized dentin discs had the capacity to attract PO43- through electrostatic interaction.

Bottom Line: The in situ mineralization within dentinal tubules is a promising treatment for dentin hypersensitivity as it induces the formation of mineral on the sensitive regions and occludes the dentinal tubules.These results showed that G3.0 PAMAM dendrimers coated on dentin surface and infiltrated in dentinal tubules could induce hydroxyapatite formation and resulted in effective dentinal tubule occlusion.This could lead to the development of a new therapeutic technique for the treatment of dentin hypersensitivity.

View Article: PubMed Central - PubMed

Affiliation: Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing, China.

ABSTRACT
The occlusion of dentinal tubules is an effective method to alleviate the symptoms caused by dentin hypersensitivity, a significant health problem in dentistry and daily life. The in situ mineralization within dentinal tubules is a promising treatment for dentin hypersensitivity as it induces the formation of mineral on the sensitive regions and occludes the dentinal tubules. This study was carried out to evaluate the in vitro effect of a whole generation poly(amidoamine) (PAMAM) dendrimer (G3.0) on dentinal tubule occlusion by inducing mineralization within dentinal tubules. Dentin discs were treated with PAMAM dendrimers using two methods, followed by the in vitro characterization using Attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), Field emission scanning electron microscopy (FE-SEM) and Energy-Dispersive X-ray Spectroscopy (EDS). These results showed that G3.0 PAMAM dendrimers coated on dentin surface and infiltrated in dentinal tubules could induce hydroxyapatite formation and resulted in effective dentinal tubule occlusion. Moreover, crosslinked PAMAM dendrimers could induce the remineralization of demineralized dentin and thus had the potential in dentinal tubule occlusion. In this in vitro study, dentinal tubules occlusion could be achieved by using PAMAM dendrimers. This could lead to the development of a new therapeutic technique for the treatment of dentin hypersensitivity.

No MeSH data available.


Related in: MedlinePlus