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Use of poly (amidoamine) dendrimer for dentinal tubule occlusion: a preliminary study.

Wang T, Yang S, Wang L, Feng H - PLoS ONE (2015)

Bottom Line: The in situ mineralization within dentinal tubules is a promising treatment for dentin hypersensitivity as it induces the formation of mineral on the sensitive regions and occludes the dentinal tubules.These results showed that G3.0 PAMAM dendrimers coated on dentin surface and infiltrated in dentinal tubules could induce hydroxyapatite formation and resulted in effective dentinal tubule occlusion.This could lead to the development of a new therapeutic technique for the treatment of dentin hypersensitivity.

View Article: PubMed Central - PubMed

Affiliation: Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing, China.

ABSTRACT
The occlusion of dentinal tubules is an effective method to alleviate the symptoms caused by dentin hypersensitivity, a significant health problem in dentistry and daily life. The in situ mineralization within dentinal tubules is a promising treatment for dentin hypersensitivity as it induces the formation of mineral on the sensitive regions and occludes the dentinal tubules. This study was carried out to evaluate the in vitro effect of a whole generation poly(amidoamine) (PAMAM) dendrimer (G3.0) on dentinal tubule occlusion by inducing mineralization within dentinal tubules. Dentin discs were treated with PAMAM dendrimers using two methods, followed by the in vitro characterization using Attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), Field emission scanning electron microscopy (FE-SEM) and Energy-Dispersive X-ray Spectroscopy (EDS). These results showed that G3.0 PAMAM dendrimers coated on dentin surface and infiltrated in dentinal tubules could induce hydroxyapatite formation and resulted in effective dentinal tubule occlusion. Moreover, crosslinked PAMAM dendrimers could induce the remineralization of demineralized dentin and thus had the potential in dentinal tubule occlusion. In this in vitro study, dentinal tubules occlusion could be achieved by using PAMAM dendrimers. This could lead to the development of a new therapeutic technique for the treatment of dentin hypersensitivity.

No MeSH data available.


Related in: MedlinePlus

Infrared spectra (2,000–500 cm-1) of PAMAM coated dentin discs.(a) Sound dentin (black line). (b) Dentin disc coated with pure G3.0 PAMAM dendrimers for 30s (red line). (c) Dentin disc immersed in pH 5.8 PBS for 2h after it was coated with G3.0 PAMAM dendrimers (blue line). (d) Untreated dentin disc immersed in pH5.8 PBS for 2h (green line).
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pone.0124735.g001: Infrared spectra (2,000–500 cm-1) of PAMAM coated dentin discs.(a) Sound dentin (black line). (b) Dentin disc coated with pure G3.0 PAMAM dendrimers for 30s (red line). (c) Dentin disc immersed in pH 5.8 PBS for 2h after it was coated with G3.0 PAMAM dendrimers (blue line). (d) Untreated dentin disc immersed in pH5.8 PBS for 2h (green line).

Mentions: ATR-FTIR spectra of PAMAM dendrimer coated samples are shown in Fig 1. After dentin discs were coated with pure G3.0 PAMAM dendrimer, the resonances at 1648 and 1547 cm-1, assigned as amide I and amide II bands became more intense (b), compared with sound dentin (a), indicating that dendrimer molecules were successfully attached to the surface of dentin discs. After PAMAM coated dentin discs were treated with PBS, the resonances around 1000 cm-1 (1008 and 957 cm-1), attributing to PO43- were strengthened and the resonances assigned as amide I and amide II bands were weakened (c), while such performances didn’t appeared after dentin discs were immersed in PBS directly without treated with PAMAM dendrimer (d). These results indicated that the PAMAM dendrimer molecules attached to the surface of dentin discs had the capacity to attract phosphate radicals (PO43-) through electrostatic interaction. Resonances corresponding to CO32- vibration at 1410 and 1446 cm-1 revealed the existence of carbonate-substituted hydroxyapatite as the main component of dentin[24].


Use of poly (amidoamine) dendrimer for dentinal tubule occlusion: a preliminary study.

Wang T, Yang S, Wang L, Feng H - PLoS ONE (2015)

Infrared spectra (2,000–500 cm-1) of PAMAM coated dentin discs.(a) Sound dentin (black line). (b) Dentin disc coated with pure G3.0 PAMAM dendrimers for 30s (red line). (c) Dentin disc immersed in pH 5.8 PBS for 2h after it was coated with G3.0 PAMAM dendrimers (blue line). (d) Untreated dentin disc immersed in pH5.8 PBS for 2h (green line).
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4401684&req=5

pone.0124735.g001: Infrared spectra (2,000–500 cm-1) of PAMAM coated dentin discs.(a) Sound dentin (black line). (b) Dentin disc coated with pure G3.0 PAMAM dendrimers for 30s (red line). (c) Dentin disc immersed in pH 5.8 PBS for 2h after it was coated with G3.0 PAMAM dendrimers (blue line). (d) Untreated dentin disc immersed in pH5.8 PBS for 2h (green line).
Mentions: ATR-FTIR spectra of PAMAM dendrimer coated samples are shown in Fig 1. After dentin discs were coated with pure G3.0 PAMAM dendrimer, the resonances at 1648 and 1547 cm-1, assigned as amide I and amide II bands became more intense (b), compared with sound dentin (a), indicating that dendrimer molecules were successfully attached to the surface of dentin discs. After PAMAM coated dentin discs were treated with PBS, the resonances around 1000 cm-1 (1008 and 957 cm-1), attributing to PO43- were strengthened and the resonances assigned as amide I and amide II bands were weakened (c), while such performances didn’t appeared after dentin discs were immersed in PBS directly without treated with PAMAM dendrimer (d). These results indicated that the PAMAM dendrimer molecules attached to the surface of dentin discs had the capacity to attract phosphate radicals (PO43-) through electrostatic interaction. Resonances corresponding to CO32- vibration at 1410 and 1446 cm-1 revealed the existence of carbonate-substituted hydroxyapatite as the main component of dentin[24].

Bottom Line: The in situ mineralization within dentinal tubules is a promising treatment for dentin hypersensitivity as it induces the formation of mineral on the sensitive regions and occludes the dentinal tubules.These results showed that G3.0 PAMAM dendrimers coated on dentin surface and infiltrated in dentinal tubules could induce hydroxyapatite formation and resulted in effective dentinal tubule occlusion.This could lead to the development of a new therapeutic technique for the treatment of dentin hypersensitivity.

View Article: PubMed Central - PubMed

Affiliation: Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing, China.

ABSTRACT
The occlusion of dentinal tubules is an effective method to alleviate the symptoms caused by dentin hypersensitivity, a significant health problem in dentistry and daily life. The in situ mineralization within dentinal tubules is a promising treatment for dentin hypersensitivity as it induces the formation of mineral on the sensitive regions and occludes the dentinal tubules. This study was carried out to evaluate the in vitro effect of a whole generation poly(amidoamine) (PAMAM) dendrimer (G3.0) on dentinal tubule occlusion by inducing mineralization within dentinal tubules. Dentin discs were treated with PAMAM dendrimers using two methods, followed by the in vitro characterization using Attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), Field emission scanning electron microscopy (FE-SEM) and Energy-Dispersive X-ray Spectroscopy (EDS). These results showed that G3.0 PAMAM dendrimers coated on dentin surface and infiltrated in dentinal tubules could induce hydroxyapatite formation and resulted in effective dentinal tubule occlusion. Moreover, crosslinked PAMAM dendrimers could induce the remineralization of demineralized dentin and thus had the potential in dentinal tubule occlusion. In this in vitro study, dentinal tubules occlusion could be achieved by using PAMAM dendrimers. This could lead to the development of a new therapeutic technique for the treatment of dentin hypersensitivity.

No MeSH data available.


Related in: MedlinePlus