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Evaluation of the diagnostic accuracy of CareStart G6PD deficiency Rapid Diagnostic Test (RDT) in a malaria endemic area in Ghana, Africa.

Adu-Gyasi D, Asante KP, Newton S, Dosoo D, Amoako S, Adjei G, Amoako N, Ankrah L, Tchum SK, Mahama E, Agyemang V, Kayan K, Owusu-Agyei S - PLoS ONE (2015)

Bottom Line: Data entry and analysis were done using Microsoft Access 2010 and Stata Software version 12.Kintampo Health Research Centre Institutional Ethics Committee granted ethical approval.Malaria infection status had no significant (P=0.199) change on the performance of the G6PD RDT test kit compared to the "gold standard".

View Article: PubMed Central - PubMed

Affiliation: Kintampo Health Research Centre, P O Box 200, Kintampo, Brong Ahafo, Ghana.

ABSTRACT

Background: Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most widespread enzyme defect that can result in red cell breakdown under oxidative stress when exposed to certain medicines including antimalarials. We evaluated the diagnostic accuracy of CareStart G6PD deficiency Rapid Diagnostic Test (RDT) as a point-of-care tool for screening G6PD deficiency.

Methods: A cross-sectional study was conducted among 206 randomly selected and consented participants from a group with known G6PD deficiency status between February 2013 and June 2013. A maximum of 1.6ml of capillary blood samples were used for G6PD deficiency screening using CareStart G6PD RDT and Trinity qualitative with Trinity quantitative methods as the "gold standard". Samples were also screened for the presence of malaria parasites. Data entry and analysis were done using Microsoft Access 2010 and Stata Software version 12. Kintampo Health Research Centre Institutional Ethics Committee granted ethical approval.

Results: The sensitivity (SE) and specificity (SP) of CareStart G6PD deficiency RDT was 100% and 72.1% compared to Trinity quantitative method respectively and was 98.9% and 96.2% compared to Trinity qualitative method. Malaria infection status had no significant (P=0.199) change on the performance of the G6PD RDT test kit compared to the "gold standard".

Conclusions: The outcome of this study suggests that the diagnostic performance of the CareStart G6PD deficiency RDT kit was high and it is acceptable at determining the G6PD deficiency status in a high malaria endemic area in Ghana. The RDT kit presents as an attractive tool for point-of-care G6PD deficiency for rapid testing in areas with high temperatures and less expertise. The CareStart G6PD deficiency RDT kit could be used to screen malaria patients before administration of the fixed dose primaquine with artemisinin-based combination therapy.

No MeSH data available.


Related in: MedlinePlus

Map of the study communities.
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pone.0125796.g001: Map of the study communities.

Mentions: The study was carried out in the Kintampo North Municipal and Kintampo South District of Ghana (Fig 1); two administrative areas where the population is periodically followed for health indicator data [10]. The study site covers an area of 7162 Sq Km with a resident population of approximately 140,000 [10]. The study area is located within the forest-savannah transitional ecological zone in Ghana and subsistent farming is the predominant occupation. The prevalence of malaria parasitemia is about 50% among children less than 10 years of age (symptomatic/asymptomatic) with perennial malaria transmission and entomological inoculation rate of 269 infective bites per person per year [7]. The prevalence of G6PD deficiency in the community is about 7% to 19% [1, 11]. There are more than twelve (12) clinics and three (3) hospitals within the study area. None of these health facilities performs the G6PD deficiency screening test as part of health service delivery except the research institution. Malaria rapid diagnostic test are however available at all health facilities and also used by community based health officers for malaria diagnosis.


Evaluation of the diagnostic accuracy of CareStart G6PD deficiency Rapid Diagnostic Test (RDT) in a malaria endemic area in Ghana, Africa.

Adu-Gyasi D, Asante KP, Newton S, Dosoo D, Amoako S, Adjei G, Amoako N, Ankrah L, Tchum SK, Mahama E, Agyemang V, Kayan K, Owusu-Agyei S - PLoS ONE (2015)

Map of the study communities.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4401677&req=5

pone.0125796.g001: Map of the study communities.
Mentions: The study was carried out in the Kintampo North Municipal and Kintampo South District of Ghana (Fig 1); two administrative areas where the population is periodically followed for health indicator data [10]. The study site covers an area of 7162 Sq Km with a resident population of approximately 140,000 [10]. The study area is located within the forest-savannah transitional ecological zone in Ghana and subsistent farming is the predominant occupation. The prevalence of malaria parasitemia is about 50% among children less than 10 years of age (symptomatic/asymptomatic) with perennial malaria transmission and entomological inoculation rate of 269 infective bites per person per year [7]. The prevalence of G6PD deficiency in the community is about 7% to 19% [1, 11]. There are more than twelve (12) clinics and three (3) hospitals within the study area. None of these health facilities performs the G6PD deficiency screening test as part of health service delivery except the research institution. Malaria rapid diagnostic test are however available at all health facilities and also used by community based health officers for malaria diagnosis.

Bottom Line: Data entry and analysis were done using Microsoft Access 2010 and Stata Software version 12.Kintampo Health Research Centre Institutional Ethics Committee granted ethical approval.Malaria infection status had no significant (P=0.199) change on the performance of the G6PD RDT test kit compared to the "gold standard".

View Article: PubMed Central - PubMed

Affiliation: Kintampo Health Research Centre, P O Box 200, Kintampo, Brong Ahafo, Ghana.

ABSTRACT

Background: Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most widespread enzyme defect that can result in red cell breakdown under oxidative stress when exposed to certain medicines including antimalarials. We evaluated the diagnostic accuracy of CareStart G6PD deficiency Rapid Diagnostic Test (RDT) as a point-of-care tool for screening G6PD deficiency.

Methods: A cross-sectional study was conducted among 206 randomly selected and consented participants from a group with known G6PD deficiency status between February 2013 and June 2013. A maximum of 1.6ml of capillary blood samples were used for G6PD deficiency screening using CareStart G6PD RDT and Trinity qualitative with Trinity quantitative methods as the "gold standard". Samples were also screened for the presence of malaria parasites. Data entry and analysis were done using Microsoft Access 2010 and Stata Software version 12. Kintampo Health Research Centre Institutional Ethics Committee granted ethical approval.

Results: The sensitivity (SE) and specificity (SP) of CareStart G6PD deficiency RDT was 100% and 72.1% compared to Trinity quantitative method respectively and was 98.9% and 96.2% compared to Trinity qualitative method. Malaria infection status had no significant (P=0.199) change on the performance of the G6PD RDT test kit compared to the "gold standard".

Conclusions: The outcome of this study suggests that the diagnostic performance of the CareStart G6PD deficiency RDT kit was high and it is acceptable at determining the G6PD deficiency status in a high malaria endemic area in Ghana. The RDT kit presents as an attractive tool for point-of-care G6PD deficiency for rapid testing in areas with high temperatures and less expertise. The CareStart G6PD deficiency RDT kit could be used to screen malaria patients before administration of the fixed dose primaquine with artemisinin-based combination therapy.

No MeSH data available.


Related in: MedlinePlus