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Transcript dynamics at early stages of molecular interactions of MYMIV with resistant and susceptible genotypes of the leguminous host, Vigna mungo.

Kundu A, Patel A, Paul S, Pal A - PLoS ONE (2015)

Bottom Line: A significant fraction of modulated transcripts are of unknown function indicating participation of novel candidate genes in restricting this viral pathogen.T9 is perhaps due to the poor execution of these transcript modulation exhibiting remarkable repression of photosynthesis related genes resulting in chlorosis of leaves followed by penalty in crop yield.In addition to inflate the existing knowledge base, the genomic resources identified in this orphan crop would be useful for integrating MYMIV-tolerance trait in susceptible cultivars of V. mungo.

View Article: PubMed Central - PubMed

Affiliation: Division of Plant Biology, Bose Institute, Kolkata 700054, West Bengal, India.

ABSTRACT
Initial phases of the MYMIV-Vigna mungo interaction is crucial in determining the infection phenotype upon challenging with the virus. During incompatible interaction, the plant deploys multiple stratagems that include extensive transcriptional alterations defying the virulence factors of the pathogen. Such molecular events are not frequently addressed by genomic tools. In order to obtain a critical insight to unravel how V. mungo respond to Mungbean yellow mosaic India virus (MYMIV), we have employed the PCR based suppression subtractive hybridization technique to identify genes that exhibit altered expressions. Dynamics of 345 candidate genes are illustrated that differentially expressed either in compatible or incompatible reactions and their possible biological and cellular functions are predicted. The MYMIV-induced physiological aspects of the resistant host include reactive oxygen species generation, induction of Ca2+ mediated signaling, enhanced expression of transcripts involved in phenylpropanoid and ubiquitin-proteasomal pathways; all these together confer resistance against the invader. Elicitation of genes implicated in salicylic acid (SA) pathway suggests that immune response is under the regulation of SA signaling. A significant fraction of modulated transcripts are of unknown function indicating participation of novel candidate genes in restricting this viral pathogen. Susceptibility on the other hand, as exhibited by V. mungo Cv. T9 is perhaps due to the poor execution of these transcript modulation exhibiting remarkable repression of photosynthesis related genes resulting in chlorosis of leaves followed by penalty in crop yield. Thus, the present findings revealed an insight on the molecular warfare during host-virus interaction suggesting plausible signaling mechanisms and key biochemical pathways overriding MYMIV invasion in resistant genotype of V. mungo. In addition to inflate the existing knowledge base, the genomic resources identified in this orphan crop would be useful for integrating MYMIV-tolerance trait in susceptible cultivars of V. mungo.

No MeSH data available.


Related in: MedlinePlus

Qualitative (A) and quantitative (B) estimation of H2O2 in V. mungo leaves (VMR84 and T9) exposed to MYMIV and mock inoculated plants.DAB staining procedure was used to visualize the in vivo accumulation of H2O2 at 0, 12, 24 and 48 hpi that correlate with the intensity of the brown colour. Bars represent mean ± standard deviation; bars followed by different letters indicate significant differences at p ≤ 0.05 according to DMRT.
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pone.0124687.g005: Qualitative (A) and quantitative (B) estimation of H2O2 in V. mungo leaves (VMR84 and T9) exposed to MYMIV and mock inoculated plants.DAB staining procedure was used to visualize the in vivo accumulation of H2O2 at 0, 12, 24 and 48 hpi that correlate with the intensity of the brown colour. Bars represent mean ± standard deviation; bars followed by different letters indicate significant differences at p ≤ 0.05 according to DMRT.

Mentions: Abundance of transcripts in the SSH libraries regulating ROS homeostasis prompted us to detect and quantify endogenous H2O2 levels by DAB infiltration procedures in pathogen inoculated tissues. Examined leaves showed presence of basal levels of H2O2 and there is not much difference in colouration in susceptible and resistant host at 0 hpi (Fig 5A and 5B). However, time-course study revealed cumulative accumulation of H2O2 producing intense colouration in resistant host, VMR84. Quantitative analysis revealed 7.5 mmol g-l FW of H2O2 at 48 hpi; while detectable level was observed from 12 hpi onwards. In contrast, staining intensity at the site of pathogenic invasion was less prominent in the susceptible reaction exhibiting weaker accumulation. Changes in expression of ROS regulatory transcripts, in both the circumstances, are essential to cope with the amplified ROS levels maintaining a state of balance and initiating systemic redox signaling upon pathogenic invasion.


Transcript dynamics at early stages of molecular interactions of MYMIV with resistant and susceptible genotypes of the leguminous host, Vigna mungo.

Kundu A, Patel A, Paul S, Pal A - PLoS ONE (2015)

Qualitative (A) and quantitative (B) estimation of H2O2 in V. mungo leaves (VMR84 and T9) exposed to MYMIV and mock inoculated plants.DAB staining procedure was used to visualize the in vivo accumulation of H2O2 at 0, 12, 24 and 48 hpi that correlate with the intensity of the brown colour. Bars represent mean ± standard deviation; bars followed by different letters indicate significant differences at p ≤ 0.05 according to DMRT.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4401676&req=5

pone.0124687.g005: Qualitative (A) and quantitative (B) estimation of H2O2 in V. mungo leaves (VMR84 and T9) exposed to MYMIV and mock inoculated plants.DAB staining procedure was used to visualize the in vivo accumulation of H2O2 at 0, 12, 24 and 48 hpi that correlate with the intensity of the brown colour. Bars represent mean ± standard deviation; bars followed by different letters indicate significant differences at p ≤ 0.05 according to DMRT.
Mentions: Abundance of transcripts in the SSH libraries regulating ROS homeostasis prompted us to detect and quantify endogenous H2O2 levels by DAB infiltration procedures in pathogen inoculated tissues. Examined leaves showed presence of basal levels of H2O2 and there is not much difference in colouration in susceptible and resistant host at 0 hpi (Fig 5A and 5B). However, time-course study revealed cumulative accumulation of H2O2 producing intense colouration in resistant host, VMR84. Quantitative analysis revealed 7.5 mmol g-l FW of H2O2 at 48 hpi; while detectable level was observed from 12 hpi onwards. In contrast, staining intensity at the site of pathogenic invasion was less prominent in the susceptible reaction exhibiting weaker accumulation. Changes in expression of ROS regulatory transcripts, in both the circumstances, are essential to cope with the amplified ROS levels maintaining a state of balance and initiating systemic redox signaling upon pathogenic invasion.

Bottom Line: A significant fraction of modulated transcripts are of unknown function indicating participation of novel candidate genes in restricting this viral pathogen.T9 is perhaps due to the poor execution of these transcript modulation exhibiting remarkable repression of photosynthesis related genes resulting in chlorosis of leaves followed by penalty in crop yield.In addition to inflate the existing knowledge base, the genomic resources identified in this orphan crop would be useful for integrating MYMIV-tolerance trait in susceptible cultivars of V. mungo.

View Article: PubMed Central - PubMed

Affiliation: Division of Plant Biology, Bose Institute, Kolkata 700054, West Bengal, India.

ABSTRACT
Initial phases of the MYMIV-Vigna mungo interaction is crucial in determining the infection phenotype upon challenging with the virus. During incompatible interaction, the plant deploys multiple stratagems that include extensive transcriptional alterations defying the virulence factors of the pathogen. Such molecular events are not frequently addressed by genomic tools. In order to obtain a critical insight to unravel how V. mungo respond to Mungbean yellow mosaic India virus (MYMIV), we have employed the PCR based suppression subtractive hybridization technique to identify genes that exhibit altered expressions. Dynamics of 345 candidate genes are illustrated that differentially expressed either in compatible or incompatible reactions and their possible biological and cellular functions are predicted. The MYMIV-induced physiological aspects of the resistant host include reactive oxygen species generation, induction of Ca2+ mediated signaling, enhanced expression of transcripts involved in phenylpropanoid and ubiquitin-proteasomal pathways; all these together confer resistance against the invader. Elicitation of genes implicated in salicylic acid (SA) pathway suggests that immune response is under the regulation of SA signaling. A significant fraction of modulated transcripts are of unknown function indicating participation of novel candidate genes in restricting this viral pathogen. Susceptibility on the other hand, as exhibited by V. mungo Cv. T9 is perhaps due to the poor execution of these transcript modulation exhibiting remarkable repression of photosynthesis related genes resulting in chlorosis of leaves followed by penalty in crop yield. Thus, the present findings revealed an insight on the molecular warfare during host-virus interaction suggesting plausible signaling mechanisms and key biochemical pathways overriding MYMIV invasion in resistant genotype of V. mungo. In addition to inflate the existing knowledge base, the genomic resources identified in this orphan crop would be useful for integrating MYMIV-tolerance trait in susceptible cultivars of V. mungo.

No MeSH data available.


Related in: MedlinePlus