Limits...
Transcript dynamics at early stages of molecular interactions of MYMIV with resistant and susceptible genotypes of the leguminous host, Vigna mungo.

Kundu A, Patel A, Paul S, Pal A - PLoS ONE (2015)

Bottom Line: A significant fraction of modulated transcripts are of unknown function indicating participation of novel candidate genes in restricting this viral pathogen.T9 is perhaps due to the poor execution of these transcript modulation exhibiting remarkable repression of photosynthesis related genes resulting in chlorosis of leaves followed by penalty in crop yield.In addition to inflate the existing knowledge base, the genomic resources identified in this orphan crop would be useful for integrating MYMIV-tolerance trait in susceptible cultivars of V. mungo.

View Article: PubMed Central - PubMed

Affiliation: Division of Plant Biology, Bose Institute, Kolkata 700054, West Bengal, India.

ABSTRACT
Initial phases of the MYMIV-Vigna mungo interaction is crucial in determining the infection phenotype upon challenging with the virus. During incompatible interaction, the plant deploys multiple stratagems that include extensive transcriptional alterations defying the virulence factors of the pathogen. Such molecular events are not frequently addressed by genomic tools. In order to obtain a critical insight to unravel how V. mungo respond to Mungbean yellow mosaic India virus (MYMIV), we have employed the PCR based suppression subtractive hybridization technique to identify genes that exhibit altered expressions. Dynamics of 345 candidate genes are illustrated that differentially expressed either in compatible or incompatible reactions and their possible biological and cellular functions are predicted. The MYMIV-induced physiological aspects of the resistant host include reactive oxygen species generation, induction of Ca2+ mediated signaling, enhanced expression of transcripts involved in phenylpropanoid and ubiquitin-proteasomal pathways; all these together confer resistance against the invader. Elicitation of genes implicated in salicylic acid (SA) pathway suggests that immune response is under the regulation of SA signaling. A significant fraction of modulated transcripts are of unknown function indicating participation of novel candidate genes in restricting this viral pathogen. Susceptibility on the other hand, as exhibited by V. mungo Cv. T9 is perhaps due to the poor execution of these transcript modulation exhibiting remarkable repression of photosynthesis related genes resulting in chlorosis of leaves followed by penalty in crop yield. Thus, the present findings revealed an insight on the molecular warfare during host-virus interaction suggesting plausible signaling mechanisms and key biochemical pathways overriding MYMIV invasion in resistant genotype of V. mungo. In addition to inflate the existing knowledge base, the genomic resources identified in this orphan crop would be useful for integrating MYMIV-tolerance trait in susceptible cultivars of V. mungo.

No MeSH data available.


Related in: MedlinePlus

Experimental design for SSH analyses after artificially inoculating resistant (VMR84) and susceptible (T9) V. mungo genotypes with MYMIV.cDNAs of virus inoculated plants were subtracted in both forward and reverse directions against mock inoculated samples infected with aviruliferous whiteflies. RNA was sampled in replicates at 3, 6, 9, 12, 18, 24, 36 and 48 hpi. Leaf tissues were also sampled for qPCR experiments at the same sampling periods excluding 9 and 18 hpi.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4401676&req=5

pone.0124687.g001: Experimental design for SSH analyses after artificially inoculating resistant (VMR84) and susceptible (T9) V. mungo genotypes with MYMIV.cDNAs of virus inoculated plants were subtracted in both forward and reverse directions against mock inoculated samples infected with aviruliferous whiteflies. RNA was sampled in replicates at 3, 6, 9, 12, 18, 24, 36 and 48 hpi. Leaf tissues were also sampled for qPCR experiments at the same sampling periods excluding 9 and 18 hpi.

Mentions: Experiments were conducted using the experimental design as outlined in Fig 1. to identify genes differentially expressed during incompatible and compatible reactions. Leaves being the feeding sites of whiteflies are considered as the primary site for MYMIV perception, where a signaling cascade initiates expression of genes in response to the recognition of foreign intruder. Two V. mungo cultivars, T9 and VMR84 were selected on the basis of their contrasting responses to MYMIV for this purpose. Both the genotypes were artificially challenged with MYMIV and subtracted in both directions from the respective mock controls, generating forward and reverse SSH libraries for each genotype following the method given below.


Transcript dynamics at early stages of molecular interactions of MYMIV with resistant and susceptible genotypes of the leguminous host, Vigna mungo.

Kundu A, Patel A, Paul S, Pal A - PLoS ONE (2015)

Experimental design for SSH analyses after artificially inoculating resistant (VMR84) and susceptible (T9) V. mungo genotypes with MYMIV.cDNAs of virus inoculated plants were subtracted in both forward and reverse directions against mock inoculated samples infected with aviruliferous whiteflies. RNA was sampled in replicates at 3, 6, 9, 12, 18, 24, 36 and 48 hpi. Leaf tissues were also sampled for qPCR experiments at the same sampling periods excluding 9 and 18 hpi.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4401676&req=5

pone.0124687.g001: Experimental design for SSH analyses after artificially inoculating resistant (VMR84) and susceptible (T9) V. mungo genotypes with MYMIV.cDNAs of virus inoculated plants were subtracted in both forward and reverse directions against mock inoculated samples infected with aviruliferous whiteflies. RNA was sampled in replicates at 3, 6, 9, 12, 18, 24, 36 and 48 hpi. Leaf tissues were also sampled for qPCR experiments at the same sampling periods excluding 9 and 18 hpi.
Mentions: Experiments were conducted using the experimental design as outlined in Fig 1. to identify genes differentially expressed during incompatible and compatible reactions. Leaves being the feeding sites of whiteflies are considered as the primary site for MYMIV perception, where a signaling cascade initiates expression of genes in response to the recognition of foreign intruder. Two V. mungo cultivars, T9 and VMR84 were selected on the basis of their contrasting responses to MYMIV for this purpose. Both the genotypes were artificially challenged with MYMIV and subtracted in both directions from the respective mock controls, generating forward and reverse SSH libraries for each genotype following the method given below.

Bottom Line: A significant fraction of modulated transcripts are of unknown function indicating participation of novel candidate genes in restricting this viral pathogen.T9 is perhaps due to the poor execution of these transcript modulation exhibiting remarkable repression of photosynthesis related genes resulting in chlorosis of leaves followed by penalty in crop yield.In addition to inflate the existing knowledge base, the genomic resources identified in this orphan crop would be useful for integrating MYMIV-tolerance trait in susceptible cultivars of V. mungo.

View Article: PubMed Central - PubMed

Affiliation: Division of Plant Biology, Bose Institute, Kolkata 700054, West Bengal, India.

ABSTRACT
Initial phases of the MYMIV-Vigna mungo interaction is crucial in determining the infection phenotype upon challenging with the virus. During incompatible interaction, the plant deploys multiple stratagems that include extensive transcriptional alterations defying the virulence factors of the pathogen. Such molecular events are not frequently addressed by genomic tools. In order to obtain a critical insight to unravel how V. mungo respond to Mungbean yellow mosaic India virus (MYMIV), we have employed the PCR based suppression subtractive hybridization technique to identify genes that exhibit altered expressions. Dynamics of 345 candidate genes are illustrated that differentially expressed either in compatible or incompatible reactions and their possible biological and cellular functions are predicted. The MYMIV-induced physiological aspects of the resistant host include reactive oxygen species generation, induction of Ca2+ mediated signaling, enhanced expression of transcripts involved in phenylpropanoid and ubiquitin-proteasomal pathways; all these together confer resistance against the invader. Elicitation of genes implicated in salicylic acid (SA) pathway suggests that immune response is under the regulation of SA signaling. A significant fraction of modulated transcripts are of unknown function indicating participation of novel candidate genes in restricting this viral pathogen. Susceptibility on the other hand, as exhibited by V. mungo Cv. T9 is perhaps due to the poor execution of these transcript modulation exhibiting remarkable repression of photosynthesis related genes resulting in chlorosis of leaves followed by penalty in crop yield. Thus, the present findings revealed an insight on the molecular warfare during host-virus interaction suggesting plausible signaling mechanisms and key biochemical pathways overriding MYMIV invasion in resistant genotype of V. mungo. In addition to inflate the existing knowledge base, the genomic resources identified in this orphan crop would be useful for integrating MYMIV-tolerance trait in susceptible cultivars of V. mungo.

No MeSH data available.


Related in: MedlinePlus