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Differential gene expression in foxtail millet during incompatible interaction with Uromyces setariae-italicae.

Li ZY, Wang N, Dong L, Bai H, Quan JZ, Liu L, Dong ZP - PLoS ONE (2015)

Bottom Line: In this study, we determined the most abundant differentially expressed signaling pathways of up-regulated genes in foxtail millet and identified significantly up-regulated genes.Expression levels of the genes were also compared between a resistant cultivar Shilixiang and a susceptible cultivar Yugu-1, and the result indicated that expression level of Shilixiang is higher than that of Yugu-1.This study reveals the relatively comprehensive mechanisms of rust-responsive transcription in foxtail millet.

View Article: PubMed Central - PubMed

Affiliation: Department of plant protect, Millet Institute, Hebei Academy of Agricultural and Forestry Sciences, National Foxtail Millet Improvement Center, Minor Cereal Crops Laboratory of Hebei Province, Shijiazhuang, China.

ABSTRACT
Foxtail millet (Setaria italica) is an important food and fodder grain crop that is grown for human consumption. Production of this species is affected by several plant diseases, such as rust. The cultivar Shilixiang has been identified as resistant to the foxtail millet rust pathogen, Uromyces setariae-italicae. In order to identify signaling pathways and genes related to the plant's defense mechanisms against rust, the Shilixiang cultivar was used to construct a digital gene expression (DGE) library during the interaction of foxtail millet with U. setariae-italicae. In this study, we determined the most abundant differentially expressed signaling pathways of up-regulated genes in foxtail millet and identified significantly up-regulated genes. Finally, quantitative real-time polymerase chain reaction (qRT-PCR) analysis was used to analyze the expression of nine selected genes, and the patterns observed agreed well with DGE analysis. Expression levels of the genes were also compared between a resistant cultivar Shilixiang and a susceptible cultivar Yugu-1, and the result indicated that expression level of Shilixiang is higher than that of Yugu-1. This study reveals the relatively comprehensive mechanisms of rust-responsive transcription in foxtail millet.

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Gene expression level comparison between resistant cultivar Shilixiang and susceptible cultivar Yugu 1.The X axis indicates the inoculation time. The Y axis indicates the fold change of target gene in qRT-PCR. SLX represent resistant cultivar Shilixiang and YG-1 represent susceptible cultivar Yugu 1. Bars represent standard errors of the means.
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pone.0123825.g007: Gene expression level comparison between resistant cultivar Shilixiang and susceptible cultivar Yugu 1.The X axis indicates the inoculation time. The Y axis indicates the fold change of target gene in qRT-PCR. SLX represent resistant cultivar Shilixiang and YG-1 represent susceptible cultivar Yugu 1. Bars represent standard errors of the means.

Mentions: Additionally, in order to determine whether the genes contribute to resistance to millet rust, expression levels of the genes were compared between the resistant cultivar Shilixiang and the susceptible cultivar Yugu-1. Results indicated that the genes can be induced in both cultivars, but with differences in their levels of gene expression: the expression level was higher in the resistant cultivar (Fig 7).


Differential gene expression in foxtail millet during incompatible interaction with Uromyces setariae-italicae.

Li ZY, Wang N, Dong L, Bai H, Quan JZ, Liu L, Dong ZP - PLoS ONE (2015)

Gene expression level comparison between resistant cultivar Shilixiang and susceptible cultivar Yugu 1.The X axis indicates the inoculation time. The Y axis indicates the fold change of target gene in qRT-PCR. SLX represent resistant cultivar Shilixiang and YG-1 represent susceptible cultivar Yugu 1. Bars represent standard errors of the means.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4401669&req=5

pone.0123825.g007: Gene expression level comparison between resistant cultivar Shilixiang and susceptible cultivar Yugu 1.The X axis indicates the inoculation time. The Y axis indicates the fold change of target gene in qRT-PCR. SLX represent resistant cultivar Shilixiang and YG-1 represent susceptible cultivar Yugu 1. Bars represent standard errors of the means.
Mentions: Additionally, in order to determine whether the genes contribute to resistance to millet rust, expression levels of the genes were compared between the resistant cultivar Shilixiang and the susceptible cultivar Yugu-1. Results indicated that the genes can be induced in both cultivars, but with differences in their levels of gene expression: the expression level was higher in the resistant cultivar (Fig 7).

Bottom Line: In this study, we determined the most abundant differentially expressed signaling pathways of up-regulated genes in foxtail millet and identified significantly up-regulated genes.Expression levels of the genes were also compared between a resistant cultivar Shilixiang and a susceptible cultivar Yugu-1, and the result indicated that expression level of Shilixiang is higher than that of Yugu-1.This study reveals the relatively comprehensive mechanisms of rust-responsive transcription in foxtail millet.

View Article: PubMed Central - PubMed

Affiliation: Department of plant protect, Millet Institute, Hebei Academy of Agricultural and Forestry Sciences, National Foxtail Millet Improvement Center, Minor Cereal Crops Laboratory of Hebei Province, Shijiazhuang, China.

ABSTRACT
Foxtail millet (Setaria italica) is an important food and fodder grain crop that is grown for human consumption. Production of this species is affected by several plant diseases, such as rust. The cultivar Shilixiang has been identified as resistant to the foxtail millet rust pathogen, Uromyces setariae-italicae. In order to identify signaling pathways and genes related to the plant's defense mechanisms against rust, the Shilixiang cultivar was used to construct a digital gene expression (DGE) library during the interaction of foxtail millet with U. setariae-italicae. In this study, we determined the most abundant differentially expressed signaling pathways of up-regulated genes in foxtail millet and identified significantly up-regulated genes. Finally, quantitative real-time polymerase chain reaction (qRT-PCR) analysis was used to analyze the expression of nine selected genes, and the patterns observed agreed well with DGE analysis. Expression levels of the genes were also compared between a resistant cultivar Shilixiang and a susceptible cultivar Yugu-1, and the result indicated that expression level of Shilixiang is higher than that of Yugu-1. This study reveals the relatively comprehensive mechanisms of rust-responsive transcription in foxtail millet.

Show MeSH
Related in: MedlinePlus