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High prevalence of HTLV-1 infection among Japanese immigrants in non-endemic area of Brazil.

Bandeira LM, Uehara SN, Asato MA, Aguena GS, Maedo CM, Benites NH, Puga MA, Rezende GR, Finotti CM, Cesar GA, Tanaka TS, Castro VO, Otsuki K, Vicente AC, Fernandes CE, Motta-Castro AR - PLoS Negl Trop Dis (2015)

Bottom Line: Descriptive analysis of behavioral risk factors showed statistical association between HTLV-1 and age greater than or equal to 45 years.Of these, 14 were sequenced and classified as Cosmopolitan subtype, and 50% (7/14) belonged to subgroup A (transcontinental) and 50% (7/14) to the subgroup B (Japanese).The high prevalence of HTLV-1 found evidence of the importance of early diagnosis and counseling of individuals infected with HTLV-1 for the control and prevention of the spread of this infection among Japanese immigrants and their descendants in Central Brazil.

View Article: PubMed Central - PubMed

Affiliation: Federal University of Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil.

ABSTRACT

Background: Human T-lymphotropic virus type 1 (HTLV-1) has worldwide distribution and is considered endemic in many world regions, including southwestern Japan and Brazil. Japanese immigrants and their descendants have a high risk of acquiring this infection due to intense population exchange between Brazil and Japan.

Objective: This cross-sectional study aimed to estimate the prevalence of HTLV, analyze the main risk factors associated with this infection, identify the main circulating types and subtypes of HTLV in Japanese immigrants and descendants living in Campo Grande-MS (Middle-West Brazil), as well as analyze the phylogenetic relationship among isolates of HTLV.

Study design: A total of 219 individuals were interviewed and submitted to blood collection. All collected blood samples were submitted for detection of anti-HTLV-1/2 using the immunoassay ELISA and confirmed by immunoblot method. The proviral DNA of the 14 samples HTLV- 1 positive were genotyped by nucleotide sequencing.

Results: The overall prevalence of HTLV-1 was 6.8% (IC 95%: 3,5-10,2). Descriptive analysis of behavioral risk factors showed statistical association between HTLV-1 and age greater than or equal to 45 years. The proviral DNA of HTLV-1 was detected in all HTLV-1 positive samples. Of these, 14 were sequenced and classified as Cosmopolitan subtype, and 50% (7/14) belonged to subgroup A (transcontinental) and 50% (7/14) to the subgroup B (Japanese).

Conclusion: The high prevalence of HTLV-1 found evidence of the importance of early diagnosis and counseling of individuals infected with HTLV-1 for the control and prevention of the spread of this infection among Japanese immigrants and their descendants in Central Brazil.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic tree constructed by neighbor-joining (NJ) method using the Kimura-2-parameters distance model for the partial LTR region of 635bp of HTLV-1 showing the phylogenetic relationships among 14 isolates of proviral DNA of HTLV-1 studied (OKW) with 15 isolates available in GenBank.
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pntd.0003691.g002: Phylogenetic tree constructed by neighbor-joining (NJ) method using the Kimura-2-parameters distance model for the partial LTR region of 635bp of HTLV-1 showing the phylogenetic relationships among 14 isolates of proviral DNA of HTLV-1 studied (OKW) with 15 isolates available in GenBank.

Mentions: The proviral DNA of HTLV-1 was detected by amplification of the 5 'LTR region by nested PCR in all samples (n = 15) HTLV-1 positive. Of them, 14 were sequenced and classified as Cosmopolitan subtype and 50% (7/14) belonged to subgroup A (Transcontinental) and 50% (7 / 14) to the subgroup B (Japanese). The new nucleotide sequence samples described in this study have been deposited in GenBank with accession number as follows: OKW21 (KM023750), OKW24 (KM023751), OKW46 (KM023752), OKW63 (KM023753), OKW72 (KM023764), OKW84 (KM023754), OKW107 (KM023761), OKW112 (KM023755), OKW131 (KM023756), OKW151 (KM023757), OKW152 (KM023758), OKW165 (KM023759), OKW209 (KM023760), OKW235(KM023767) were compared with nucleotide sequences of 17 isolates of HTLV-1 available from GenBank (Fig 2). The GenBank accession numbers for the sequences of the 5 'LTR region of HTLV-1 included in the phylogenetic analysis are: ATK1 (J02029), H5 (M37299), pyg19 (L76310), ITIS (Z32527), Me3 (Y16480), CA423 (EU108724), Mel5 (Lo2534), K344 (GQ443755), BRLO14-02 (JF271836), BRRP438 (DQ323811), Qu3 (Y16477), 1066/05 (HQ606137), 526MZ (GU194504), Ni2 (Y16487), efe1 (Y17014). The HTLV-1aA strains of this study clustered closely with other isolates from Latin America, mainly from Brazil. Further, the HTLV-1aB strains of this study clustered closely with other isolates from Japan and from a Japanese descendant from Peru.


High prevalence of HTLV-1 infection among Japanese immigrants in non-endemic area of Brazil.

Bandeira LM, Uehara SN, Asato MA, Aguena GS, Maedo CM, Benites NH, Puga MA, Rezende GR, Finotti CM, Cesar GA, Tanaka TS, Castro VO, Otsuki K, Vicente AC, Fernandes CE, Motta-Castro AR - PLoS Negl Trop Dis (2015)

Phylogenetic tree constructed by neighbor-joining (NJ) method using the Kimura-2-parameters distance model for the partial LTR region of 635bp of HTLV-1 showing the phylogenetic relationships among 14 isolates of proviral DNA of HTLV-1 studied (OKW) with 15 isolates available in GenBank.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4401538&req=5

pntd.0003691.g002: Phylogenetic tree constructed by neighbor-joining (NJ) method using the Kimura-2-parameters distance model for the partial LTR region of 635bp of HTLV-1 showing the phylogenetic relationships among 14 isolates of proviral DNA of HTLV-1 studied (OKW) with 15 isolates available in GenBank.
Mentions: The proviral DNA of HTLV-1 was detected by amplification of the 5 'LTR region by nested PCR in all samples (n = 15) HTLV-1 positive. Of them, 14 were sequenced and classified as Cosmopolitan subtype and 50% (7/14) belonged to subgroup A (Transcontinental) and 50% (7 / 14) to the subgroup B (Japanese). The new nucleotide sequence samples described in this study have been deposited in GenBank with accession number as follows: OKW21 (KM023750), OKW24 (KM023751), OKW46 (KM023752), OKW63 (KM023753), OKW72 (KM023764), OKW84 (KM023754), OKW107 (KM023761), OKW112 (KM023755), OKW131 (KM023756), OKW151 (KM023757), OKW152 (KM023758), OKW165 (KM023759), OKW209 (KM023760), OKW235(KM023767) were compared with nucleotide sequences of 17 isolates of HTLV-1 available from GenBank (Fig 2). The GenBank accession numbers for the sequences of the 5 'LTR region of HTLV-1 included in the phylogenetic analysis are: ATK1 (J02029), H5 (M37299), pyg19 (L76310), ITIS (Z32527), Me3 (Y16480), CA423 (EU108724), Mel5 (Lo2534), K344 (GQ443755), BRLO14-02 (JF271836), BRRP438 (DQ323811), Qu3 (Y16477), 1066/05 (HQ606137), 526MZ (GU194504), Ni2 (Y16487), efe1 (Y17014). The HTLV-1aA strains of this study clustered closely with other isolates from Latin America, mainly from Brazil. Further, the HTLV-1aB strains of this study clustered closely with other isolates from Japan and from a Japanese descendant from Peru.

Bottom Line: Descriptive analysis of behavioral risk factors showed statistical association between HTLV-1 and age greater than or equal to 45 years.Of these, 14 were sequenced and classified as Cosmopolitan subtype, and 50% (7/14) belonged to subgroup A (transcontinental) and 50% (7/14) to the subgroup B (Japanese).The high prevalence of HTLV-1 found evidence of the importance of early diagnosis and counseling of individuals infected with HTLV-1 for the control and prevention of the spread of this infection among Japanese immigrants and their descendants in Central Brazil.

View Article: PubMed Central - PubMed

Affiliation: Federal University of Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil.

ABSTRACT

Background: Human T-lymphotropic virus type 1 (HTLV-1) has worldwide distribution and is considered endemic in many world regions, including southwestern Japan and Brazil. Japanese immigrants and their descendants have a high risk of acquiring this infection due to intense population exchange between Brazil and Japan.

Objective: This cross-sectional study aimed to estimate the prevalence of HTLV, analyze the main risk factors associated with this infection, identify the main circulating types and subtypes of HTLV in Japanese immigrants and descendants living in Campo Grande-MS (Middle-West Brazil), as well as analyze the phylogenetic relationship among isolates of HTLV.

Study design: A total of 219 individuals were interviewed and submitted to blood collection. All collected blood samples were submitted for detection of anti-HTLV-1/2 using the immunoassay ELISA and confirmed by immunoblot method. The proviral DNA of the 14 samples HTLV- 1 positive were genotyped by nucleotide sequencing.

Results: The overall prevalence of HTLV-1 was 6.8% (IC 95%: 3,5-10,2). Descriptive analysis of behavioral risk factors showed statistical association between HTLV-1 and age greater than or equal to 45 years. The proviral DNA of HTLV-1 was detected in all HTLV-1 positive samples. Of these, 14 were sequenced and classified as Cosmopolitan subtype, and 50% (7/14) belonged to subgroup A (transcontinental) and 50% (7/14) to the subgroup B (Japanese).

Conclusion: The high prevalence of HTLV-1 found evidence of the importance of early diagnosis and counseling of individuals infected with HTLV-1 for the control and prevention of the spread of this infection among Japanese immigrants and their descendants in Central Brazil.

No MeSH data available.


Related in: MedlinePlus