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Physiological and proteomics analyses reveal the mechanism of Eichhornia crassipes tolerance to high-concentration cadmium stress compared with Pistia stratiotes.

Li X, Zhou Y, Yang Y, Yang S, Sun X, Yang Y - PLoS ONE (2015)

Bottom Line: Cadmium (Cd) pollution is an environmental problem worldwide.As a result, E. crassipes could maintain more stable physiological parameters than P. stratiotes.In addition, antioxidant enzymes played important roles in ROS detoxification.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; China Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China; University of Chinese Academy of Sciences, Beijing 100049, China.

ABSTRACT
Cadmium (Cd) pollution is an environmental problem worldwide. Phytoremediation is a convenient method of removing Cd from both soil and water, but its efficiency is still low, especially in aquatic environments. Scientists have been trying to improve the ability of plants to absorb and accumulate Cd based on interactions between plants and Cd, especially the mechanism by which plants resist Cd. Eichhornia crassipes and Pistia stratiotes are aquatic plants commonly used in the phytoremediation of heavy metals. In the present study, we conducted physiological and biochemical analyses to compare the resistance of these two species to Cd stress at 100 mg/L. E. crassipes showed stronger resistance and was therefore used for subsequent comparative proteomics to explore the potential mechanism of E. crassipes tolerance to Cd stress at the protein level. The expression patterns of proteins in different functional categories revealed that the physiological activities and metabolic processes of E. crassipes were affected by exposure to Cd stress. However, when some proteins related to these processes were negatively inhibited, some analogous proteins were induced to compensate for the corresponding functions. As a result, E. crassipes could maintain more stable physiological parameters than P. stratiotes. Many stress-resistance substances and proteins, such as proline and heat shock proteins (HSPs) and post translational modifications, were found to be involved in the protection and repair of functional proteins. In addition, antioxidant enzymes played important roles in ROS detoxification. These findings will facilitate further understanding of the potential mechanism of plant response to Cd stress at the protein level.

No MeSH data available.


Related in: MedlinePlus

Changes in antioxidant enzyme activity in E. crassipes and P. stratiotes exposed to 100 mg/L Cd for different times.A: CAT activity. B: APX activity. C: GR activity. D: SOD activity. Data are presented as mean ± standard error. Different letters following mean values indicate significant differences (Tukey’s test, P<0.05).
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pone.0124304.g004: Changes in antioxidant enzyme activity in E. crassipes and P. stratiotes exposed to 100 mg/L Cd for different times.A: CAT activity. B: APX activity. C: GR activity. D: SOD activity. Data are presented as mean ± standard error. Different letters following mean values indicate significant differences (Tukey’s test, P<0.05).

Mentions: To investigate the role of antioxidant system in regulating ROS accumulation, we measured the activities of four common antioxidant enzymes. The activities of catalase (CAT; EC 1.11.1.6), ascorbate peroxidase (APX; EC 1.11.1.11), glutathione reductase (GR; EC 1.8.1.7), and superoxide dismutase (SOD; EC 1.15.1.1) in E. crassipes were consistently much higher than in P. stratiotes (Fig 4), and they all increased significantly with increasing treatment time in E. crassipes (Fig 4). However, antioxidant enzyme activities first increased, then decreased in P. stratiotes (Fig 4). The maximum CAT, APX, and SOD activities were observed at 3 d, after which they began to decrease (Fig 4A, 4B and 4D). The highest GR activity was observed following exposure to Cd stress for 2 d (Fig 4C).


Physiological and proteomics analyses reveal the mechanism of Eichhornia crassipes tolerance to high-concentration cadmium stress compared with Pistia stratiotes.

Li X, Zhou Y, Yang Y, Yang S, Sun X, Yang Y - PLoS ONE (2015)

Changes in antioxidant enzyme activity in E. crassipes and P. stratiotes exposed to 100 mg/L Cd for different times.A: CAT activity. B: APX activity. C: GR activity. D: SOD activity. Data are presented as mean ± standard error. Different letters following mean values indicate significant differences (Tukey’s test, P<0.05).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4401520&req=5

pone.0124304.g004: Changes in antioxidant enzyme activity in E. crassipes and P. stratiotes exposed to 100 mg/L Cd for different times.A: CAT activity. B: APX activity. C: GR activity. D: SOD activity. Data are presented as mean ± standard error. Different letters following mean values indicate significant differences (Tukey’s test, P<0.05).
Mentions: To investigate the role of antioxidant system in regulating ROS accumulation, we measured the activities of four common antioxidant enzymes. The activities of catalase (CAT; EC 1.11.1.6), ascorbate peroxidase (APX; EC 1.11.1.11), glutathione reductase (GR; EC 1.8.1.7), and superoxide dismutase (SOD; EC 1.15.1.1) in E. crassipes were consistently much higher than in P. stratiotes (Fig 4), and they all increased significantly with increasing treatment time in E. crassipes (Fig 4). However, antioxidant enzyme activities first increased, then decreased in P. stratiotes (Fig 4). The maximum CAT, APX, and SOD activities were observed at 3 d, after which they began to decrease (Fig 4A, 4B and 4D). The highest GR activity was observed following exposure to Cd stress for 2 d (Fig 4C).

Bottom Line: Cadmium (Cd) pollution is an environmental problem worldwide.As a result, E. crassipes could maintain more stable physiological parameters than P. stratiotes.In addition, antioxidant enzymes played important roles in ROS detoxification.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; China Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China; University of Chinese Academy of Sciences, Beijing 100049, China.

ABSTRACT
Cadmium (Cd) pollution is an environmental problem worldwide. Phytoremediation is a convenient method of removing Cd from both soil and water, but its efficiency is still low, especially in aquatic environments. Scientists have been trying to improve the ability of plants to absorb and accumulate Cd based on interactions between plants and Cd, especially the mechanism by which plants resist Cd. Eichhornia crassipes and Pistia stratiotes are aquatic plants commonly used in the phytoremediation of heavy metals. In the present study, we conducted physiological and biochemical analyses to compare the resistance of these two species to Cd stress at 100 mg/L. E. crassipes showed stronger resistance and was therefore used for subsequent comparative proteomics to explore the potential mechanism of E. crassipes tolerance to Cd stress at the protein level. The expression patterns of proteins in different functional categories revealed that the physiological activities and metabolic processes of E. crassipes were affected by exposure to Cd stress. However, when some proteins related to these processes were negatively inhibited, some analogous proteins were induced to compensate for the corresponding functions. As a result, E. crassipes could maintain more stable physiological parameters than P. stratiotes. Many stress-resistance substances and proteins, such as proline and heat shock proteins (HSPs) and post translational modifications, were found to be involved in the protection and repair of functional proteins. In addition, antioxidant enzymes played important roles in ROS detoxification. These findings will facilitate further understanding of the potential mechanism of plant response to Cd stress at the protein level.

No MeSH data available.


Related in: MedlinePlus