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Physiological and proteomics analyses reveal the mechanism of Eichhornia crassipes tolerance to high-concentration cadmium stress compared with Pistia stratiotes.

Li X, Zhou Y, Yang Y, Yang S, Sun X, Yang Y - PLoS ONE (2015)

Bottom Line: Cadmium (Cd) pollution is an environmental problem worldwide.As a result, E. crassipes could maintain more stable physiological parameters than P. stratiotes.In addition, antioxidant enzymes played important roles in ROS detoxification.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; China Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China; University of Chinese Academy of Sciences, Beijing 100049, China.

ABSTRACT
Cadmium (Cd) pollution is an environmental problem worldwide. Phytoremediation is a convenient method of removing Cd from both soil and water, but its efficiency is still low, especially in aquatic environments. Scientists have been trying to improve the ability of plants to absorb and accumulate Cd based on interactions between plants and Cd, especially the mechanism by which plants resist Cd. Eichhornia crassipes and Pistia stratiotes are aquatic plants commonly used in the phytoremediation of heavy metals. In the present study, we conducted physiological and biochemical analyses to compare the resistance of these two species to Cd stress at 100 mg/L. E. crassipes showed stronger resistance and was therefore used for subsequent comparative proteomics to explore the potential mechanism of E. crassipes tolerance to Cd stress at the protein level. The expression patterns of proteins in different functional categories revealed that the physiological activities and metabolic processes of E. crassipes were affected by exposure to Cd stress. However, when some proteins related to these processes were negatively inhibited, some analogous proteins were induced to compensate for the corresponding functions. As a result, E. crassipes could maintain more stable physiological parameters than P. stratiotes. Many stress-resistance substances and proteins, such as proline and heat shock proteins (HSPs) and post translational modifications, were found to be involved in the protection and repair of functional proteins. In addition, antioxidant enzymes played important roles in ROS detoxification. These findings will facilitate further understanding of the potential mechanism of plant response to Cd stress at the protein level.

No MeSH data available.


Related in: MedlinePlus

Changes in morphology and chlorophyll fluorescence of E. crassipes and P. stratiotes exposed to 100 mg/L Cd for different times.A: Changes in leaf and root morphology. B: Fv/Fm images. The pseudocolor code depicted at the top of the image ranges from 0 (red) to 1 (purple). C: Average Fv/Fm values. Data are the means ± SE. Different letters following mean values indicate significant differences (Tukey’s test, P<0.05).
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pone.0124304.g001: Changes in morphology and chlorophyll fluorescence of E. crassipes and P. stratiotes exposed to 100 mg/L Cd for different times.A: Changes in leaf and root morphology. B: Fv/Fm images. The pseudocolor code depicted at the top of the image ranges from 0 (red) to 1 (purple). C: Average Fv/Fm values. Data are the means ± SE. Different letters following mean values indicate significant differences (Tukey’s test, P<0.05).

Mentions: We first observed the morphological change to see the different resistance to high-concentration Cd between E. crassipes and P. stratiotes. Under Cd stress, the leaves of E. crassipes began to droop, but showed no discoloration or withering with increased treatment time (Fig 1A). However, the leaves of P. stratiotes turned yellow and withered from the leaf edges as the treatment time increased, and they began to fall off after 5 d of treatment (Fig 1A). Greater differences were observed in the roots relative to the leaves. Specifically, the roots of E. crassipes showed no obvious changes in response to Cd exposure until 5 d of treatment (Fig 1A), at which point the lateral roots began to fall. However, the roots of P. stratiotes started falling after Cd exposure for 2 d (Fig 1A), and they became rotten after 5 d of treatment (Fig 1A).


Physiological and proteomics analyses reveal the mechanism of Eichhornia crassipes tolerance to high-concentration cadmium stress compared with Pistia stratiotes.

Li X, Zhou Y, Yang Y, Yang S, Sun X, Yang Y - PLoS ONE (2015)

Changes in morphology and chlorophyll fluorescence of E. crassipes and P. stratiotes exposed to 100 mg/L Cd for different times.A: Changes in leaf and root morphology. B: Fv/Fm images. The pseudocolor code depicted at the top of the image ranges from 0 (red) to 1 (purple). C: Average Fv/Fm values. Data are the means ± SE. Different letters following mean values indicate significant differences (Tukey’s test, P<0.05).
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4401520&req=5

pone.0124304.g001: Changes in morphology and chlorophyll fluorescence of E. crassipes and P. stratiotes exposed to 100 mg/L Cd for different times.A: Changes in leaf and root morphology. B: Fv/Fm images. The pseudocolor code depicted at the top of the image ranges from 0 (red) to 1 (purple). C: Average Fv/Fm values. Data are the means ± SE. Different letters following mean values indicate significant differences (Tukey’s test, P<0.05).
Mentions: We first observed the morphological change to see the different resistance to high-concentration Cd between E. crassipes and P. stratiotes. Under Cd stress, the leaves of E. crassipes began to droop, but showed no discoloration or withering with increased treatment time (Fig 1A). However, the leaves of P. stratiotes turned yellow and withered from the leaf edges as the treatment time increased, and they began to fall off after 5 d of treatment (Fig 1A). Greater differences were observed in the roots relative to the leaves. Specifically, the roots of E. crassipes showed no obvious changes in response to Cd exposure until 5 d of treatment (Fig 1A), at which point the lateral roots began to fall. However, the roots of P. stratiotes started falling after Cd exposure for 2 d (Fig 1A), and they became rotten after 5 d of treatment (Fig 1A).

Bottom Line: Cadmium (Cd) pollution is an environmental problem worldwide.As a result, E. crassipes could maintain more stable physiological parameters than P. stratiotes.In addition, antioxidant enzymes played important roles in ROS detoxification.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; China Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China; University of Chinese Academy of Sciences, Beijing 100049, China.

ABSTRACT
Cadmium (Cd) pollution is an environmental problem worldwide. Phytoremediation is a convenient method of removing Cd from both soil and water, but its efficiency is still low, especially in aquatic environments. Scientists have been trying to improve the ability of plants to absorb and accumulate Cd based on interactions between plants and Cd, especially the mechanism by which plants resist Cd. Eichhornia crassipes and Pistia stratiotes are aquatic plants commonly used in the phytoremediation of heavy metals. In the present study, we conducted physiological and biochemical analyses to compare the resistance of these two species to Cd stress at 100 mg/L. E. crassipes showed stronger resistance and was therefore used for subsequent comparative proteomics to explore the potential mechanism of E. crassipes tolerance to Cd stress at the protein level. The expression patterns of proteins in different functional categories revealed that the physiological activities and metabolic processes of E. crassipes were affected by exposure to Cd stress. However, when some proteins related to these processes were negatively inhibited, some analogous proteins were induced to compensate for the corresponding functions. As a result, E. crassipes could maintain more stable physiological parameters than P. stratiotes. Many stress-resistance substances and proteins, such as proline and heat shock proteins (HSPs) and post translational modifications, were found to be involved in the protection and repair of functional proteins. In addition, antioxidant enzymes played important roles in ROS detoxification. These findings will facilitate further understanding of the potential mechanism of plant response to Cd stress at the protein level.

No MeSH data available.


Related in: MedlinePlus