Stem cell conditioned culture media attenuated albumin-induced epithelial-mesenchymal transition in renal tubular cells.
Bottom Line: Albumin induced EMT as shown by significant decreases in levels of epithelial marker E-cadherin, increases in mesenchymal markers fibroblast-specific protein 1 and α-smooth muscle actin, and elevations in collagen I.Albumin also increased the levels of pro-inflammatory factor monocyte chemoattractant protein-1 (MCP)-1 by nearly 30 fold compared with control.SCM almost abolished albumin-induced increase of MCP-1.
Affiliation: Department of Pharmacology & Toxicology, Virginia Commonwealth University School of Medicine, Richmond, VA, USA.Show MeSH
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Mentions: MCP-1 is one of the important inflammatory mediators. The levels of MCP-1 mRNA and protein expression were significantly higher in albumin-treated cells than that in control. However, in cells treated with albumin + SCM, the MCP-1 mRNA and protein expressions were much lower than that in albumin-treated cells, similar to that in CCM-treated control cells (Fig. 8). These data suggest that SCM inhibits albumin-induced activation of pro-inflammatory factors.
Affiliation: Department of Pharmacology & Toxicology, Virginia Commonwealth University School of Medicine, Richmond, VA, USA.