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Stem cell conditioned culture media attenuated albumin-induced epithelial-mesenchymal transition in renal tubular cells.

Hu J, Zhu Q, Li PL, Wang W, Yi F, Li N - Cell. Physiol. Biochem. (2015)

Bottom Line: Albumin induced EMT as shown by significant decreases in levels of epithelial marker E-cadherin, increases in mesenchymal markers fibroblast-specific protein 1 and α-smooth muscle actin, and elevations in collagen I.Albumin also increased the levels of pro-inflammatory factor monocyte chemoattractant protein-1 (MCP)-1 by nearly 30 fold compared with control.SCM almost abolished albumin-induced increase of MCP-1.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology & Toxicology, Virginia Commonwealth University School of Medicine, Richmond, VA, USA.

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Related in: MedlinePlus

Effect of SCM on albumin-induced increase in MCP-1. Upper panel: mRNA levels by real-time RT-PCR analysis; Lower panel: Protein levels by Western blot analysis. Ctrl = control cells treated with CCM, Alb = cells treated with albumin + CCM, Alb+SCM = cells treated with albumin + SCM. n=4–6 batches of cells, *P<0.05 vs. other groups.
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Figure 8: Effect of SCM on albumin-induced increase in MCP-1. Upper panel: mRNA levels by real-time RT-PCR analysis; Lower panel: Protein levels by Western blot analysis. Ctrl = control cells treated with CCM, Alb = cells treated with albumin + CCM, Alb+SCM = cells treated with albumin + SCM. n=4–6 batches of cells, *P<0.05 vs. other groups.

Mentions: MCP-1 is one of the important inflammatory mediators. The levels of MCP-1 mRNA and protein expression were significantly higher in albumin-treated cells than that in control. However, in cells treated with albumin + SCM, the MCP-1 mRNA and protein expressions were much lower than that in albumin-treated cells, similar to that in CCM-treated control cells (Fig. 8). These data suggest that SCM inhibits albumin-induced activation of pro-inflammatory factors.


Stem cell conditioned culture media attenuated albumin-induced epithelial-mesenchymal transition in renal tubular cells.

Hu J, Zhu Q, Li PL, Wang W, Yi F, Li N - Cell. Physiol. Biochem. (2015)

Effect of SCM on albumin-induced increase in MCP-1. Upper panel: mRNA levels by real-time RT-PCR analysis; Lower panel: Protein levels by Western blot analysis. Ctrl = control cells treated with CCM, Alb = cells treated with albumin + CCM, Alb+SCM = cells treated with albumin + SCM. n=4–6 batches of cells, *P<0.05 vs. other groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4401473&req=5

Figure 8: Effect of SCM on albumin-induced increase in MCP-1. Upper panel: mRNA levels by real-time RT-PCR analysis; Lower panel: Protein levels by Western blot analysis. Ctrl = control cells treated with CCM, Alb = cells treated with albumin + CCM, Alb+SCM = cells treated with albumin + SCM. n=4–6 batches of cells, *P<0.05 vs. other groups.
Mentions: MCP-1 is one of the important inflammatory mediators. The levels of MCP-1 mRNA and protein expression were significantly higher in albumin-treated cells than that in control. However, in cells treated with albumin + SCM, the MCP-1 mRNA and protein expressions were much lower than that in albumin-treated cells, similar to that in CCM-treated control cells (Fig. 8). These data suggest that SCM inhibits albumin-induced activation of pro-inflammatory factors.

Bottom Line: Albumin induced EMT as shown by significant decreases in levels of epithelial marker E-cadherin, increases in mesenchymal markers fibroblast-specific protein 1 and α-smooth muscle actin, and elevations in collagen I.Albumin also increased the levels of pro-inflammatory factor monocyte chemoattractant protein-1 (MCP)-1 by nearly 30 fold compared with control.SCM almost abolished albumin-induced increase of MCP-1.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology & Toxicology, Virginia Commonwealth University School of Medicine, Richmond, VA, USA.

Show MeSH
Related in: MedlinePlus