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Stem cell conditioned culture media attenuated albumin-induced epithelial-mesenchymal transition in renal tubular cells.

Hu J, Zhu Q, Li PL, Wang W, Yi F, Li N - Cell. Physiol. Biochem. (2015)

Bottom Line: Albumin induced EMT as shown by significant decreases in levels of epithelial marker E-cadherin, increases in mesenchymal markers fibroblast-specific protein 1 and α-smooth muscle actin, and elevations in collagen I.Albumin also increased the levels of pro-inflammatory factor monocyte chemoattractant protein-1 (MCP)-1 by nearly 30 fold compared with control.SCM almost abolished albumin-induced increase of MCP-1.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology & Toxicology, Virginia Commonwealth University School of Medicine, Richmond, VA, USA.

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Related in: MedlinePlus

Effect of SCM on albumin-induced changes of staining patterns in NF-κB-p65 by immunofluorescent microscopy assay. Representative confocal images showing the immunostaining of nuclear marker (green) and NF-κB-p65 (red) (from 5 batches of cells). Control = control cells treated with CCM, Albumin = cells treated with albumin + CCM, Albumin+SCM = cells treated with albumin + SCM.
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Figure 7: Effect of SCM on albumin-induced changes of staining patterns in NF-κB-p65 by immunofluorescent microscopy assay. Representative confocal images showing the immunostaining of nuclear marker (green) and NF-κB-p65 (red) (from 5 batches of cells). Control = control cells treated with CCM, Albumin = cells treated with albumin + CCM, Albumin+SCM = cells treated with albumin + SCM.

Mentions: Effects of SCM on albumin-induced NF-κB translocation into nuclei were further investigated by fluorescence confocal images. As shown in Fig. 7, NF-κB-p65 staining was mainly located in cytoplasm with a weak staining in nuclei in most of CCM-treated control cells, whereas most of nuclei showed a strong NF-κB-p65 staining in albumin + CCM-treated cells, indicating a nuclear translocation of NF-κB. Overlaid images showed a much stronger yellow color in the nuclei in albumin-treated cells than that in control cells, further indicating a nuclear translocation of NF-κB. However, staining pattern of NF-κB-p65 in cells treated with albumin + SCM was similar to that in CCM-treated control cells. These results were consistent with the above findings by western blot assay, further suggesting that albumin-induced nuclear translocation of NF-κB were blocked by SCM treatment.


Stem cell conditioned culture media attenuated albumin-induced epithelial-mesenchymal transition in renal tubular cells.

Hu J, Zhu Q, Li PL, Wang W, Yi F, Li N - Cell. Physiol. Biochem. (2015)

Effect of SCM on albumin-induced changes of staining patterns in NF-κB-p65 by immunofluorescent microscopy assay. Representative confocal images showing the immunostaining of nuclear marker (green) and NF-κB-p65 (red) (from 5 batches of cells). Control = control cells treated with CCM, Albumin = cells treated with albumin + CCM, Albumin+SCM = cells treated with albumin + SCM.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4401473&req=5

Figure 7: Effect of SCM on albumin-induced changes of staining patterns in NF-κB-p65 by immunofluorescent microscopy assay. Representative confocal images showing the immunostaining of nuclear marker (green) and NF-κB-p65 (red) (from 5 batches of cells). Control = control cells treated with CCM, Albumin = cells treated with albumin + CCM, Albumin+SCM = cells treated with albumin + SCM.
Mentions: Effects of SCM on albumin-induced NF-κB translocation into nuclei were further investigated by fluorescence confocal images. As shown in Fig. 7, NF-κB-p65 staining was mainly located in cytoplasm with a weak staining in nuclei in most of CCM-treated control cells, whereas most of nuclei showed a strong NF-κB-p65 staining in albumin + CCM-treated cells, indicating a nuclear translocation of NF-κB. Overlaid images showed a much stronger yellow color in the nuclei in albumin-treated cells than that in control cells, further indicating a nuclear translocation of NF-κB. However, staining pattern of NF-κB-p65 in cells treated with albumin + SCM was similar to that in CCM-treated control cells. These results were consistent with the above findings by western blot assay, further suggesting that albumin-induced nuclear translocation of NF-κB were blocked by SCM treatment.

Bottom Line: Albumin induced EMT as shown by significant decreases in levels of epithelial marker E-cadherin, increases in mesenchymal markers fibroblast-specific protein 1 and α-smooth muscle actin, and elevations in collagen I.Albumin also increased the levels of pro-inflammatory factor monocyte chemoattractant protein-1 (MCP)-1 by nearly 30 fold compared with control.SCM almost abolished albumin-induced increase of MCP-1.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology & Toxicology, Virginia Commonwealth University School of Medicine, Richmond, VA, USA.

Show MeSH
Related in: MedlinePlus