Stem cell conditioned culture media attenuated albumin-induced epithelial-mesenchymal transition in renal tubular cells.
Bottom Line: Albumin induced EMT as shown by significant decreases in levels of epithelial marker E-cadherin, increases in mesenchymal markers fibroblast-specific protein 1 and α-smooth muscle actin, and elevations in collagen I.Albumin also increased the levels of pro-inflammatory factor monocyte chemoattractant protein-1 (MCP)-1 by nearly 30 fold compared with control.SCM almost abolished albumin-induced increase of MCP-1.
Affiliation: Department of Pharmacology & Toxicology, Virginia Commonwealth University School of Medicine, Richmond, VA, USA.Show MeSH
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Mentions: Effects of SCM on albumin-induced NF-κB translocation into nuclei were further investigated by fluorescence confocal images. As shown in Fig. 7, NF-κB-p65 staining was mainly located in cytoplasm with a weak staining in nuclei in most of CCM-treated control cells, whereas most of nuclei showed a strong NF-κB-p65 staining in albumin + CCM-treated cells, indicating a nuclear translocation of NF-κB. Overlaid images showed a much stronger yellow color in the nuclei in albumin-treated cells than that in control cells, further indicating a nuclear translocation of NF-κB. However, staining pattern of NF-κB-p65 in cells treated with albumin + SCM was similar to that in CCM-treated control cells. These results were consistent with the above findings by western blot assay, further suggesting that albumin-induced nuclear translocation of NF-κB were blocked by SCM treatment.
Affiliation: Department of Pharmacology & Toxicology, Virginia Commonwealth University School of Medicine, Richmond, VA, USA.