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Stem cell conditioned culture media attenuated albumin-induced epithelial-mesenchymal transition in renal tubular cells.

Hu J, Zhu Q, Li PL, Wang W, Yi F, Li N - Cell. Physiol. Biochem. (2015)

Bottom Line: Albumin induced EMT as shown by significant decreases in levels of epithelial marker E-cadherin, increases in mesenchymal markers fibroblast-specific protein 1 and α-smooth muscle actin, and elevations in collagen I.Albumin also increased the levels of pro-inflammatory factor monocyte chemoattractant protein-1 (MCP)-1 by nearly 30 fold compared with control.SCM almost abolished albumin-induced increase of MCP-1.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology & Toxicology, Virginia Commonwealth University School of Medicine, Richmond, VA, USA.

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Effect of SCM on albumin-induced increase in NF-κB-p65 in nuclear proteins by Western blot analysis. Upper panel: Representative gel documents; Lower panel: Summarized data. Ctrl = control cells treated with CCM, Alb = cells treated with albumin + CCM, Alb+SCM = cells treated with albumin + SCM. n=6 batches of cells, *P<0.05 vs. other groups.
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Figure 6: Effect of SCM on albumin-induced increase in NF-κB-p65 in nuclear proteins by Western blot analysis. Upper panel: Representative gel documents; Lower panel: Summarized data. Ctrl = control cells treated with CCM, Alb = cells treated with albumin + CCM, Alb+SCM = cells treated with albumin + SCM. n=6 batches of cells, *P<0.05 vs. other groups.

Mentions: NF-κB plays a key role in regulating inflammation. It has been reported that NF-κB is involved in TGF-β-induced EMT [26]. Our Western blot results showed that NF-κB-p65 levels in nuclear protein were significantly higher in cells treated with albumin + CCM than in cells treated with CCM. However, the NF-κB-p65 levels were much lower in cells treated with albumin + SCM than in cell treated with albumin + CCM, similar to that in CCM-treated control cells (Fig. 6).


Stem cell conditioned culture media attenuated albumin-induced epithelial-mesenchymal transition in renal tubular cells.

Hu J, Zhu Q, Li PL, Wang W, Yi F, Li N - Cell. Physiol. Biochem. (2015)

Effect of SCM on albumin-induced increase in NF-κB-p65 in nuclear proteins by Western blot analysis. Upper panel: Representative gel documents; Lower panel: Summarized data. Ctrl = control cells treated with CCM, Alb = cells treated with albumin + CCM, Alb+SCM = cells treated with albumin + SCM. n=6 batches of cells, *P<0.05 vs. other groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4401473&req=5

Figure 6: Effect of SCM on albumin-induced increase in NF-κB-p65 in nuclear proteins by Western blot analysis. Upper panel: Representative gel documents; Lower panel: Summarized data. Ctrl = control cells treated with CCM, Alb = cells treated with albumin + CCM, Alb+SCM = cells treated with albumin + SCM. n=6 batches of cells, *P<0.05 vs. other groups.
Mentions: NF-κB plays a key role in regulating inflammation. It has been reported that NF-κB is involved in TGF-β-induced EMT [26]. Our Western blot results showed that NF-κB-p65 levels in nuclear protein were significantly higher in cells treated with albumin + CCM than in cells treated with CCM. However, the NF-κB-p65 levels were much lower in cells treated with albumin + SCM than in cell treated with albumin + CCM, similar to that in CCM-treated control cells (Fig. 6).

Bottom Line: Albumin induced EMT as shown by significant decreases in levels of epithelial marker E-cadherin, increases in mesenchymal markers fibroblast-specific protein 1 and α-smooth muscle actin, and elevations in collagen I.Albumin also increased the levels of pro-inflammatory factor monocyte chemoattractant protein-1 (MCP)-1 by nearly 30 fold compared with control.SCM almost abolished albumin-induced increase of MCP-1.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology & Toxicology, Virginia Commonwealth University School of Medicine, Richmond, VA, USA.

Show MeSH
Related in: MedlinePlus