Stem cell conditioned culture media attenuated albumin-induced epithelial-mesenchymal transition in renal tubular cells.
Bottom Line: Albumin induced EMT as shown by significant decreases in levels of epithelial marker E-cadherin, increases in mesenchymal markers fibroblast-specific protein 1 and α-smooth muscle actin, and elevations in collagen I.Albumin also increased the levels of pro-inflammatory factor monocyte chemoattractant protein-1 (MCP)-1 by nearly 30 fold compared with control.SCM almost abolished albumin-induced increase of MCP-1.
Affiliation: Department of Pharmacology & Toxicology, Virginia Commonwealth University School of Medicine, Richmond, VA, USA.Show MeSH
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Mentions: NF-κB plays a key role in regulating inflammation. It has been reported that NF-κB is involved in TGF-β-induced EMT . Our Western blot results showed that NF-κB-p65 levels in nuclear protein were significantly higher in cells treated with albumin + CCM than in cells treated with CCM. However, the NF-κB-p65 levels were much lower in cells treated with albumin + SCM than in cell treated with albumin + CCM, similar to that in CCM-treated control cells (Fig. 6).
Affiliation: Department of Pharmacology & Toxicology, Virginia Commonwealth University School of Medicine, Richmond, VA, USA.