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Stem cell conditioned culture media attenuated albumin-induced epithelial-mesenchymal transition in renal tubular cells.

Hu J, Zhu Q, Li PL, Wang W, Yi F, Li N - Cell. Physiol. Biochem. (2015)

Bottom Line: Albumin induced EMT as shown by significant decreases in levels of epithelial marker E-cadherin, increases in mesenchymal markers fibroblast-specific protein 1 and α-smooth muscle actin, and elevations in collagen I.Albumin also increased the levels of pro-inflammatory factor monocyte chemoattractant protein-1 (MCP)-1 by nearly 30 fold compared with control.SCM almost abolished albumin-induced increase of MCP-1.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology & Toxicology, Virginia Commonwealth University School of Medicine, Richmond, VA, USA.

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Effect of SCM on albumin-induced changes of staining patterns in E-cadherin, FSP-1 and α-SMA by immunofluorescent microscopy assay. Upper panel: Representative confocal images showing the immunostaining of E-cadherin, FSP-1 and α-SMA; Lower panel: Summarized integrated optical intensity of the fluorescent staining. Ctrl = control cells treated with CCM, Alb = cells treated with albumin + CCM, Alb+SCM = cells treated with albumin + SCM. n=5 batches of cells, *P<0.05 vs. other groups.
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Figure 4: Effect of SCM on albumin-induced changes of staining patterns in E-cadherin, FSP-1 and α-SMA by immunofluorescent microscopy assay. Upper panel: Representative confocal images showing the immunostaining of E-cadherin, FSP-1 and α-SMA; Lower panel: Summarized integrated optical intensity of the fluorescent staining. Ctrl = control cells treated with CCM, Alb = cells treated with albumin + CCM, Alb+SCM = cells treated with albumin + SCM. n=5 batches of cells, *P<0.05 vs. other groups.

Mentions: To further investigate the SCM effects on the albumin-induced EMT, immune staining analysis of EMT markers were performed in cells with different treatments. As shown in Fig. 4, immunostaining of E-Cadherin clearly outlined the cell contours with enriched fluorescence along cell membrane in control cells treated with CCM; in cells treated with albumin + CCM, the intensity of immunostaining was reduced and the contour of E-Cadherin staining was discontinuous; in cells treated with albumin + SCM, the contour of E-Cadherin staining was recovered and the intensity of immunostaining was significantly increased compared with that in cells treated with albumin. In contrast, the staining of mesenchymal cell markers FSP-1 and α-SMA were both weak in control cells treated with CCM (Fig. 4); in cells treated with albumin + CCM, the staining of both FSP-1 and α-SMA was much stronger than that in control cells; in cells treated with albumin + SCM, however, the staining of both α-SMA and FSP-1 was similar to the levels in control cells. These results additionally demonstrated that SCM blocked the albumin-induced changes in EMT markers.


Stem cell conditioned culture media attenuated albumin-induced epithelial-mesenchymal transition in renal tubular cells.

Hu J, Zhu Q, Li PL, Wang W, Yi F, Li N - Cell. Physiol. Biochem. (2015)

Effect of SCM on albumin-induced changes of staining patterns in E-cadherin, FSP-1 and α-SMA by immunofluorescent microscopy assay. Upper panel: Representative confocal images showing the immunostaining of E-cadherin, FSP-1 and α-SMA; Lower panel: Summarized integrated optical intensity of the fluorescent staining. Ctrl = control cells treated with CCM, Alb = cells treated with albumin + CCM, Alb+SCM = cells treated with albumin + SCM. n=5 batches of cells, *P<0.05 vs. other groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4401473&req=5

Figure 4: Effect of SCM on albumin-induced changes of staining patterns in E-cadherin, FSP-1 and α-SMA by immunofluorescent microscopy assay. Upper panel: Representative confocal images showing the immunostaining of E-cadherin, FSP-1 and α-SMA; Lower panel: Summarized integrated optical intensity of the fluorescent staining. Ctrl = control cells treated with CCM, Alb = cells treated with albumin + CCM, Alb+SCM = cells treated with albumin + SCM. n=5 batches of cells, *P<0.05 vs. other groups.
Mentions: To further investigate the SCM effects on the albumin-induced EMT, immune staining analysis of EMT markers were performed in cells with different treatments. As shown in Fig. 4, immunostaining of E-Cadherin clearly outlined the cell contours with enriched fluorescence along cell membrane in control cells treated with CCM; in cells treated with albumin + CCM, the intensity of immunostaining was reduced and the contour of E-Cadherin staining was discontinuous; in cells treated with albumin + SCM, the contour of E-Cadherin staining was recovered and the intensity of immunostaining was significantly increased compared with that in cells treated with albumin. In contrast, the staining of mesenchymal cell markers FSP-1 and α-SMA were both weak in control cells treated with CCM (Fig. 4); in cells treated with albumin + CCM, the staining of both FSP-1 and α-SMA was much stronger than that in control cells; in cells treated with albumin + SCM, however, the staining of both α-SMA and FSP-1 was similar to the levels in control cells. These results additionally demonstrated that SCM blocked the albumin-induced changes in EMT markers.

Bottom Line: Albumin induced EMT as shown by significant decreases in levels of epithelial marker E-cadherin, increases in mesenchymal markers fibroblast-specific protein 1 and α-smooth muscle actin, and elevations in collagen I.Albumin also increased the levels of pro-inflammatory factor monocyte chemoattractant protein-1 (MCP)-1 by nearly 30 fold compared with control.SCM almost abolished albumin-induced increase of MCP-1.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology & Toxicology, Virginia Commonwealth University School of Medicine, Richmond, VA, USA.

Show MeSH
Related in: MedlinePlus